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• W3134776196 endingPage "105046" @default.
• W3134776196 startingPage "105046" @default.
• W3134776196 abstract "The capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2O2) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2O2-induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7." @default.
• W3134776196 created "2021-03-15" @default.
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• W3134776196 date "2021-07-01" @default.
• W3134776196 modified "2023-09-27" @default.
• W3134776196 title "Effect of skimmed milk on intestinal tract: Prevention of increased reactive oxygen species and nitric oxide formation" @default.
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• W3134776196 doi "https://doi.org/10.1016/j.idairyj.2021.105046" @default.
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