Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134787169> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W3134787169 abstract "Plant arginase that catalyses the hydrolysis of arginine to ornithine and urea is known to play an important role in nitrogen metabolism. Recently, we reported a highly stable arginase from cilantro and its sensitivity to biotic and abiotic stress. During this investigation, we found horse gram also possessing a stable arginase among legumes. Hence, we partially purified arginase from horse gram seedlings by conventional chromatographic techniques with 869-fold purity, and a specific activity of 13752 nmoles of urea formed/mg of protein/min. The enzyme is relatively heat stable and requires Mn2+ for its activity and is sensitive to reducing agents and EDTA similar to cilantro arginase. The optimum pH and temperature for partially purified horse gram arginase was found to be 7.87 and 37° C - 60° C respectively. Arginine-derived polyamines and amino acids can regulate horse gram arginase in vitro. Partially purified arginase hydrolyses L-arginine and is incapable of hydrolysing other arginine analogues except L-homoarginine, a property that distinguishes horse gram arginase from cilantro arginase. The Km for partially purified arginase was found to be 5.47 ± 0.34 mM with respect to L-arginine. As plant arginases are not stable and their subunit organization differs from source to source, for further purification and biochemical characterization horse gram serves as an ideal and easily available source.
" @default.
- W3134787169 created "2021-03-15" @default.
- W3134787169 creator A5010620748 @default.
- W3134787169 creator A5044747127 @default.
- W3134787169 creator A5061703263 @default.
- W3134787169 creator A5086858098 @default.
- W3134787169 date "2021-03-22" @default.
- W3134787169 modified "2023-09-27" @default.
- W3134787169 title "Partial Purification and Biochemical Characterization of Horse Gram Arginase" @default.
- W3134787169 doi "https://doi.org/10.22376/ijpbs/lpr.2021.11.2.p68-78" @default.
- W3134787169 hasPublicationYear "2021" @default.
- W3134787169 type Work @default.
- W3134787169 sameAs 3134787169 @default.
- W3134787169 citedByCount "0" @default.
- W3134787169 crossrefType "journal-article" @default.
- W3134787169 hasAuthorship W3134787169A5010620748 @default.
- W3134787169 hasAuthorship W3134787169A5044747127 @default.
- W3134787169 hasAuthorship W3134787169A5061703263 @default.
- W3134787169 hasAuthorship W3134787169A5086858098 @default.
- W3134787169 hasBestOaLocation W31347871691 @default.
- W3134787169 hasConcept C151730666 @default.
- W3134787169 hasConcept C164007495 @default.
- W3134787169 hasConcept C181199279 @default.
- W3134787169 hasConcept C185592680 @default.
- W3134787169 hasConcept C2776796294 @default.
- W3134787169 hasConcept C2777468819 @default.
- W3134787169 hasConcept C2780365088 @default.
- W3134787169 hasConcept C2780762169 @default.
- W3134787169 hasConcept C515207424 @default.
- W3134787169 hasConcept C55493867 @default.
- W3134787169 hasConcept C77061444 @default.
- W3134787169 hasConcept C86803240 @default.
- W3134787169 hasConceptScore W3134787169C151730666 @default.
- W3134787169 hasConceptScore W3134787169C164007495 @default.
- W3134787169 hasConceptScore W3134787169C181199279 @default.
- W3134787169 hasConceptScore W3134787169C185592680 @default.
- W3134787169 hasConceptScore W3134787169C2776796294 @default.
- W3134787169 hasConceptScore W3134787169C2777468819 @default.
- W3134787169 hasConceptScore W3134787169C2780365088 @default.
- W3134787169 hasConceptScore W3134787169C2780762169 @default.
- W3134787169 hasConceptScore W3134787169C515207424 @default.
- W3134787169 hasConceptScore W3134787169C55493867 @default.
- W3134787169 hasConceptScore W3134787169C77061444 @default.
- W3134787169 hasConceptScore W3134787169C86803240 @default.
- W3134787169 hasLocation W31347871691 @default.
- W3134787169 hasOpenAccess W3134787169 @default.
- W3134787169 hasPrimaryLocation W31347871691 @default.
- W3134787169 hasRelatedWork W1835288751 @default.
- W3134787169 hasRelatedWork W1968866357 @default.
- W3134787169 hasRelatedWork W2001382272 @default.
- W3134787169 hasRelatedWork W2029982427 @default.
- W3134787169 hasRelatedWork W2068116687 @default.
- W3134787169 hasRelatedWork W2073507440 @default.
- W3134787169 hasRelatedWork W2152768879 @default.
- W3134787169 hasRelatedWork W2315069809 @default.
- W3134787169 hasRelatedWork W2412037395 @default.
- W3134787169 hasRelatedWork W2483064625 @default.
- W3134787169 isParatext "false" @default.
- W3134787169 isRetracted "false" @default.
- W3134787169 magId "3134787169" @default.
- W3134787169 workType "article" @default.