Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134799255> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W3134799255 endingPage "15" @default.
- W3134799255 startingPage "9" @default.
- W3134799255 abstract "Aim. The study was aimed at cloning and analysis of molecular organization of 5S rDNA intergenic spacer (IGS) in two Gentiana species of Ukrainian flora, G. pneumonanthe L. and G. punctata L. Methods. 5S rDNA IGS sequence was amplified using polymerase chain reaction (PCR) with a pair of primers specific for the gene coding region. The produced PCR products were fractionated by gel-electrophoresis, isolated, ligated into plasmid pUC18, cloned into E. coli, and then sequenced. Nucleotide sequences were aligned using the Muscle algorithm and analyzed in the Unipro UGENE software. Results. The intergenic spacer region of the 5S rRNA genes was cloned and sequenced for two Gentiana species of Ukrainian flora, G. pneumonanthe and G. punctata. Based on the analysis of the alignment of the IGS sequences of five Gentiana species from three sections, some features of molecular organization of IGS of 5S rRNA genes in the studied species were established. In particular, motifs typical for other angiosperm families were identified, such as conservative oligo-dT motif at the IGS 3'-end that served as a transcription termination site and AT-rich region preceding the coding region of 5S rRNA gene. However, in the region of transcription initiation, conservative GC-element in position -13 is changed to AC. Conclusions. The interspecific variation of molecular organization of 5S rDNA IGS was identified among Gentiana species that can be used to clarify the phylogenetic relationships between members of this genus.Keywords: Gentiana species, 5S rDNA intergenic spacer, molecular organization, phylogeny." @default.
- W3134799255 created "2021-03-15" @default.
- W3134799255 creator A5012150580 @default.
- W3134799255 creator A5032304983 @default.
- W3134799255 creator A5045665897 @default.
- W3134799255 creator A5069450960 @default.
- W3134799255 creator A5075862138 @default.
- W3134799255 creator A5078072416 @default.
- W3134799255 date "2021-01-29" @default.
- W3134799255 modified "2023-10-16" @default.
- W3134799255 title "Molecular organization of 5S rDNA intergenic spacer in Gentiana pneumonanthe L. and G. punctata L." @default.
- W3134799255 cites W1454903780 @default.
- W3134799255 cites W1940000715 @default.
- W3134799255 cites W1991463580 @default.
- W3134799255 cites W1993320561 @default.
- W3134799255 cites W2030764070 @default.
- W3134799255 cites W2048747971 @default.
- W3134799255 cites W2049746310 @default.
- W3134799255 cites W2062483564 @default.
- W3134799255 cites W2098466396 @default.
- W3134799255 cites W2102644314 @default.
- W3134799255 cites W2132926880 @default.
- W3134799255 cites W2133000765 @default.
- W3134799255 cites W2267583118 @default.
- W3134799255 cites W2312494664 @default.
- W3134799255 cites W2464900547 @default.
- W3134799255 cites W2560534247 @default.
- W3134799255 cites W2799524357 @default.
- W3134799255 cites W2938574745 @default.
- W3134799255 cites W3037814317 @default.
- W3134799255 cites W3041137962 @default.
- W3134799255 cites W3097744609 @default.
- W3134799255 cites W3128150609 @default.
- W3134799255 cites W38585004 @default.
- W3134799255 doi "https://doi.org/10.7124/visnyk.utgis.18.1-2.1349" @default.
- W3134799255 hasPublicationYear "2021" @default.
- W3134799255 type Work @default.
- W3134799255 sameAs 3134799255 @default.
- W3134799255 citedByCount "1" @default.
- W3134799255 countsByYear W31347992552022 @default.
- W3134799255 crossrefType "journal-article" @default.
- W3134799255 hasAuthorship W3134799255A5012150580 @default.
- W3134799255 hasAuthorship W3134799255A5032304983 @default.
- W3134799255 hasAuthorship W3134799255A5045665897 @default.
- W3134799255 hasAuthorship W3134799255A5069450960 @default.
- W3134799255 hasAuthorship W3134799255A5075862138 @default.
- W3134799255 hasAuthorship W3134799255A5078072416 @default.
- W3134799255 hasBestOaLocation W31347992551 @default.
- W3134799255 hasConcept C104317684 @default.
- W3134799255 hasConcept C141231307 @default.
- W3134799255 hasConcept C193252679 @default.
- W3134799255 hasConcept C195139083 @default.
- W3134799255 hasConcept C207812311 @default.
- W3134799255 hasConcept C2781038578 @default.
- W3134799255 hasConcept C54355233 @default.
- W3134799255 hasConcept C59822182 @default.
- W3134799255 hasConcept C67905577 @default.
- W3134799255 hasConcept C86803240 @default.
- W3134799255 hasConceptScore W3134799255C104317684 @default.
- W3134799255 hasConceptScore W3134799255C141231307 @default.
- W3134799255 hasConceptScore W3134799255C193252679 @default.
- W3134799255 hasConceptScore W3134799255C195139083 @default.
- W3134799255 hasConceptScore W3134799255C207812311 @default.
- W3134799255 hasConceptScore W3134799255C2781038578 @default.
- W3134799255 hasConceptScore W3134799255C54355233 @default.
- W3134799255 hasConceptScore W3134799255C59822182 @default.
- W3134799255 hasConceptScore W3134799255C67905577 @default.
- W3134799255 hasConceptScore W3134799255C86803240 @default.
- W3134799255 hasIssue "1-2" @default.
- W3134799255 hasLocation W31347992551 @default.
- W3134799255 hasOpenAccess W3134799255 @default.
- W3134799255 hasPrimaryLocation W31347992551 @default.
- W3134799255 hasRelatedWork W1820048409 @default.
- W3134799255 hasRelatedWork W1995225927 @default.
- W3134799255 hasRelatedWork W2012846295 @default.
- W3134799255 hasRelatedWork W2046217121 @default.
- W3134799255 hasRelatedWork W2051662717 @default.
- W3134799255 hasRelatedWork W2067243745 @default.
- W3134799255 hasRelatedWork W2084739049 @default.
- W3134799255 hasRelatedWork W3073579923 @default.
- W3134799255 hasRelatedWork W3161267193 @default.
- W3134799255 hasRelatedWork W885294193 @default.
- W3134799255 hasVolume "18" @default.
- W3134799255 isParatext "false" @default.
- W3134799255 isRetracted "false" @default.
- W3134799255 magId "3134799255" @default.
- W3134799255 workType "article" @default.