FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134822559> ?p ?o ?g. }

• W3134822559 abstract "Abstract Background PGF2α is essential for the induction of the corpus luteum regression which in turn reduces progesterone production. Early growth response (EGR) proteins are Cys2-His2-type zinc-finger transcription factor that are strongly linked to cellular proliferation, survival and apoptosis. Rapid elevation of EGR1 was observed after luteolytic dose of PGF2α. EGR1 is involved in the transactivation of many genes, including TGFβ1, which plays an important role during luteal regression. Methods The current study was conducted in buffalo luteal cells with the aim to better understand the role of EGR1 in transactivation of TGFβ1 during PGF2α induced luteal regression. Luteal cells from mid stage corpus luteum of buffalo were cultured and treated with different doses of PGF2α for different time durations. Relative expression of mRNAs encoding for enzymes within the progesterone biosynthetic pathway ( 3βHSD , CYP11A1 and StAR ); Caspase 3 ; AKT were analyzed to confirm the occurrence of luteolytic event. To determine if EGR1 is involved in the PGF2α induced luteal regression via induction of TGFβ1 expression, we knocked out the EGR1 gene by using CRISPR/Cas9. Result The present experiment determined whether EGR1 protein expression in luteal cells was responsive to PGF2α treatment. Quantification of EGR1 and TGFβ1 mRNA showed significant up regulation in luteal cells of buffalo at 12 h post PGF2α induction. In order to validate the role of PGF2α on stimulating the expression of TGFβ1 by an EGR1 dependent mechanism we knocked out EGR1. The EGR1 ablated luteal cells were stimulated with PGF2α and it was observed that EGR1 KO did not modulate the PGF2α induced expression of TGFβ1 . In PGF2α treated EGR1 KO luteal cell, the mRNA expression of Caspase 3 was significantly increased compared to PGF2α treated wild type luteal cells maintained for 12 h. We also studied the influence of EGR1 on steroidogenesis. The EGR1 KO luteal cells with PGF2α treatment showed no substantial difference either in the progesterone concentration or in StAR mRNA expression with PGF2α-treated wild type luteal cells. Conclusion These results suggest that EGR1 signaling is not the only factor which plays a role in the regulation of PGF2α induced TGFβ1 signaling for luteolysis." @default.
• W3134822559 created "2021-03-15" @default.
• W3134822559 creator A5001114885 @default.
• W3134822559 creator A5011482428 @default.
• W3134822559 creator A5012957826 @default.
• W3134822559 creator A5026175551 @default.
• W3134822559 creator A5044440842 @default.
• W3134822559 creator A5063537184 @default.
• W3134822559 creator A5074994344 @default.
• W3134822559 creator A5087807843 @default.
• W3134822559 creator A5088142677 @default.
• W3134822559 date "2021-03-12" @default.
• W3134822559 modified "2023-10-16" @default.
• W3134822559 title "Deciphering the functional role of EGR1 in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffalo" @default.
• W3134822559 cites W1527815822 @default.
• W3134822559 cites W1570035884 @default.
• W3134822559 cites W1705232837 @default.
• W3134822559 cites W1968549922 @default.
• W3134822559 cites W1974481221 @default.
• W3134822559 cites W1997693327 @default.
• W3134822559 cites W1999511788 @default.
• W3134822559 cites W2005348371 @default.
• W3134822559 cites W2007795861 @default.
• W3134822559 cites W2019519527 @default.
• W3134822559 cites W2019996430 @default.
• W3134822559 cites W2021496076 @default.
• W3134822559 cites W2029620795 @default.
• W3134822559 cites W2041937331 @default.
• W3134822559 cites W2053514790 @default.
• W3134822559 cites W2071082766 @default.
• W3134822559 cites W2080474281 @default.
• W3134822559 cites W2085013563 @default.
• W3134822559 cites W2087367550 @default.
• W3134822559 cites W2092120029 @default.
• W3134822559 cites W2092368761 @default.
• W3134822559 cites W2098195361 @default.
• W3134822559 cites W2099165943 @default.
• W3134822559 cites W2101009531 @default.
• W3134822559 cites W2103380615 @default.
• W3134822559 cites W2103536899 @default.
• W3134822559 cites W2112862426 @default.
• W3134822559 cites W2129660051 @default.
• W3134822559 cites W2136591671 @default.
• W3134822559 cites W2153724711 @default.
• W3134822559 cites W2156617735 @default.
• W3134822559 cites W2157360739 @default.
• W3134822559 cites W2163545710 @default.
• W3134822559 cites W2169184428 @default.
• W3134822559 cites W2181983966 @default.
• W3134822559 cites W2271900218 @default.
• W3134822559 cites W2514860072 @default.
• W3134822559 cites W2546569040 @default.
• W3134822559 cites W2595487480 @default.
• W3134822559 cites W2618340198 @default.
• W3134822559 cites W2903045548 @default.
• W3134822559 cites W2996850363 @default.
• W3134822559 cites W3017588238 @default.
• W3134822559 cites W333206344 @default.
• W3134822559 cites W64005057 @default.
• W3134822559 doi "https://doi.org/10.1186/s40659-021-00333-7" @default.
• W3134822559 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7953609" @default.
• W3134822559 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33712084" @default.
• W3134822559 hasPublicationYear "2021" @default.
• W3134822559 type Work @default.
• W3134822559 sameAs 3134822559 @default.
• W3134822559 citedByCount "3" @default.
• W3134822559 countsByYear W31348225592022 @default.
• W3134822559 countsByYear W31348225592023 @default.
• W3134822559 crossrefType "journal-article" @default.
• W3134822559 hasAuthorship W3134822559A5001114885 @default.
• W3134822559 hasAuthorship W3134822559A5011482428 @default.
• W3134822559 hasAuthorship W3134822559A5012957826 @default.
• W3134822559 hasAuthorship W3134822559A5026175551 @default.
• W3134822559 hasAuthorship W3134822559A5044440842 @default.
• W3134822559 hasAuthorship W3134822559A5063537184 @default.
• W3134822559 hasAuthorship W3134822559A5074994344 @default.
• W3134822559 hasAuthorship W3134822559A5087807843 @default.
• W3134822559 hasAuthorship W3134822559A5088142677 @default.
• W3134822559 hasBestOaLocation W31348225591 @default.
• W3134822559 hasConcept C104317684 @default.
• W3134822559 hasConcept C126322002 @default.
• W3134822559 hasConcept C1292079 @default.
• W3134822559 hasConcept C134018914 @default.
• W3134822559 hasConcept C143228043 @default.
• W3134822559 hasConcept C150194340 @default.
• W3134822559 hasConcept C187785154 @default.
• W3134822559 hasConcept C2776608385 @default.
• W3134822559 hasConcept C2777330693 @default.
• W3134822559 hasConcept C2778324911 @default.
• W3134822559 hasConcept C2780600689 @default.
• W3134822559 hasConcept C55493867 @default.
• W3134822559 hasConcept C71924100 @default.
• W3134822559 hasConcept C86803240 @default.
• W3134822559 hasConceptScore W3134822559C104317684 @default.
• W3134822559 hasConceptScore W3134822559C126322002 @default.
• W3134822559 hasConceptScore W3134822559C1292079 @default.
• W3134822559 hasConceptScore W3134822559C134018914 @default.
• W3134822559 hasConceptScore W3134822559C143228043 @default.
• W3134822559 hasConceptScore W3134822559C150194340 @default.
• W3134822559 hasConceptScore W3134822559C187785154 @default.

• next