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• W3134891198 endingPage "115756" @default.
• W3134891198 startingPage "115756" @default.
• W3134891198 abstract "Cholesteric liquid crystal (CLC) is characterized by the selective reflection in the Bragg condition for constructive interference. Instead of assembling a LC cell with a pair of glass substrates, only a single glass substrate modified with vertical alignment reagent and layered with a CLC film of 3.4 ± 0.2 μm in thickness was utilized in this study for the detection of bovine serum albumin (BSA). By fine-tuning the ratio of the thickness d to the helical pitch P of the CLC film, both qualitative and quantitative biosensing capabilities were demonstrated with an E7/R811 CLC (d/P close to but smaller than unity) and an E7/R5011 CLC (d/P > > 1) film, respectively. The E7/R811 film was in the unwound homeotropic state in the absence of BSA, but transitioned to the fingerprint state at 10−9 to 10−6-g/ml BSA and to the planar state between 10−5 and 10−2-g/ml BSA. Quantitative protein assay based on E7/R5011 CLCs was established through transmission spectrometric analysis, and detection sensitivity was optimized by adjusting the Bragg reflection wavelength. A limit of detection of 5.9 × 10−9 g/ml was achieved for the CLC film consisting of 2.0-wt% R5011 in the nematic host E7 and exhibiting a Bragg reflection wavelength of 700 nm. As a majority of clinical assays are performed on a single solid substrate, the CLC-based, single-substrate biosensing platform allows for better integration with current technologies. Besides, the protein array employed in this work can be further miniaturized and converted to a protein microarray format to facilitate high-throughput analysis." @default.
• W3134891198 created "2021-03-15" @default.
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• W3134891198 date "2021-06-01" @default.
• W3134891198 modified "2023-10-08" @default.
• W3134891198 title "Label-free single-substrate quantitative protein assay based on optical characteristics of cholesteric liquid crystals" @default.
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• W3134891198 doi "https://doi.org/10.1016/j.molliq.2021.115756" @default.
• W3134891198 hasPublicationYear "2021" @default.
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