FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134966512> ?p ?o ?g. }

• W3134966512 endingPage "2679" @default.
• W3134966512 startingPage "2668" @default.
• W3134966512 abstract "Abstract Methylation biomarkers are promising tools for diagnosis and disease prevention. The S5 classifier is aimed at the prevention of cervical cancer by the early detection of cervical intraepithelial neoplasia (CIN). S5 is based on pyrosequencing a promoter region of EPB41L3 and five late regions of HPV types 16, 18, 31, and 33 following bisulfite conversion of DNA. Good biomarkers should perform well in a variety of sample types such as exfoliated cells, fresh frozen or formalin‐fixed paraffin‐embedded (FFPE) materials. Here, we tested the performance of S5 on 315 FFPE biopsies with paired exfoliated cervical samples using four different conversion kits (Epitect Bisulfite, Epitect Fast Bisulfite, EZ DNA Methylation, and EZ DNA Methylation‐Lightning). The S5 values from FFPE biopsies for all kits were significantly correlated with those obtained from their paired exfoliated cells. For the EZ DNA Methylation kit, we observed an average increased methylation of 4.4% in FFPE. This was due to incomplete conversion of DNA (73% for FFPE vs. 95% for cells). The other kits had a DNA conversion rate in FFPE similar to the cells (95%–97%). S5 performed well at discriminating <CIN2 lesions from CIN2+ lesions on the FFPE with all kits given optimized adjustments to the cut‐off. The area under the curve (AUC) for S5 on FFPE was not significantly different from the paired cells (0.74–0.79 vs. 0.81). The best sensitivity and specificity were obtained for EZ DNA Methylation after the adjustment of the cut‐off to reflect its lower conversion rate. Consistent methylation results can be obtained from FFPE material regardless of the conversion kit used. The S5 classifier performed as well on FFPE material as on exfoliated cells with adjusted cut‐off allowing easier clinical implementation." @default.
• W3134966512 created "2021-03-15" @default.
• W3134966512 creator A5000979514 @default.
• W3134966512 creator A5003854603 @default.
• W3134966512 creator A5010920104 @default.
• W3134966512 creator A5022237789 @default.
• W3134966512 creator A5033719178 @default.
• W3134966512 creator A5037034501 @default.
• W3134966512 creator A5088625860 @default.
• W3134966512 date "2021-03-12" @default.
• W3134966512 modified "2023-10-06" @default.
• W3134966512 title "Consistency of the S5 DNA methylation classifier in formalin‐fixed biopsies versus corresponding exfoliated cells for the detection of pre‐cancerous cervical lesions" @default.
• W3134966512 cites W1428147504 @default.
• W3134966512 cites W1496182593 @default.
• W3134966512 cites W1955901306 @default.
• W3134966512 cites W1964783528 @default.
• W3134966512 cites W1978732622 @default.
• W3134966512 cites W2003473150 @default.
• W3134966512 cites W2025998934 @default.
• W3134966512 cites W2042016354 @default.
• W3134966512 cites W2081898372 @default.
• W3134966512 cites W2089417825 @default.
• W3134966512 cites W2097215405 @default.
• W3134966512 cites W2111248090 @default.
• W3134966512 cites W2115046713 @default.
• W3134966512 cites W2154448561 @default.
• W3134966512 cites W2252521216 @default.
• W3134966512 cites W2297701691 @default.
• W3134966512 cites W2460742292 @default.
• W3134966512 cites W2578727382 @default.
• W3134966512 cites W2615683555 @default.
• W3134966512 cites W2748019834 @default.
• W3134966512 cites W2801427790 @default.
• W3134966512 cites W2899549022 @default.
• W3134966512 cites W2963416133 @default.
• W3134966512 cites W2979311555 @default.
• W3134966512 cites W4240866925 @default.
• W3134966512 doi "https://doi.org/10.1002/cam4.3849" @default.
• W3134966512 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8026949" @default.
• W3134966512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33710792" @default.
• W3134966512 hasPublicationYear "2021" @default.
• W3134966512 type Work @default.
• W3134966512 sameAs 3134966512 @default.
• W3134966512 citedByCount "3" @default.
• W3134966512 countsByYear W31349665122021 @default.
• W3134966512 countsByYear W31349665122023 @default.
• W3134966512 crossrefType "journal-article" @default.
• W3134966512 hasAuthorship W3134966512A5000979514 @default.
• W3134966512 hasAuthorship W3134966512A5003854603 @default.
• W3134966512 hasAuthorship W3134966512A5010920104 @default.
• W3134966512 hasAuthorship W3134966512A5022237789 @default.
• W3134966512 hasAuthorship W3134966512A5033719178 @default.
• W3134966512 hasAuthorship W3134966512A5037034501 @default.
• W3134966512 hasAuthorship W3134966512A5088625860 @default.
• W3134966512 hasBestOaLocation W31349665123 @default.
• W3134966512 hasConcept C104317684 @default.
• W3134966512 hasConcept C121608353 @default.
• W3134966512 hasConcept C126322002 @default.
• W3134966512 hasConcept C142724271 @default.
• W3134966512 hasConcept C150194340 @default.
• W3134966512 hasConcept C153911025 @default.
• W3134966512 hasConcept C189235521 @default.
• W3134966512 hasConcept C190727270 @default.
• W3134966512 hasConcept C2775934546 @default.
• W3134966512 hasConcept C2775959680 @default.
• W3134966512 hasConcept C2777343196 @default.
• W3134966512 hasConcept C2778220009 @default.
• W3134966512 hasConcept C33288867 @default.
• W3134966512 hasConcept C49105822 @default.
• W3134966512 hasConcept C54355233 @default.
• W3134966512 hasConcept C552990157 @default.
• W3134966512 hasConcept C71924100 @default.
• W3134966512 hasConcept C86803240 @default.
• W3134966512 hasConceptScore W3134966512C104317684 @default.
• W3134966512 hasConceptScore W3134966512C121608353 @default.
• W3134966512 hasConceptScore W3134966512C126322002 @default.
• W3134966512 hasConceptScore W3134966512C142724271 @default.
• W3134966512 hasConceptScore W3134966512C150194340 @default.
• W3134966512 hasConceptScore W3134966512C153911025 @default.
• W3134966512 hasConceptScore W3134966512C189235521 @default.
• W3134966512 hasConceptScore W3134966512C190727270 @default.
• W3134966512 hasConceptScore W3134966512C2775934546 @default.
• W3134966512 hasConceptScore W3134966512C2775959680 @default.
• W3134966512 hasConceptScore W3134966512C2777343196 @default.
• W3134966512 hasConceptScore W3134966512C2778220009 @default.
• W3134966512 hasConceptScore W3134966512C33288867 @default.
• W3134966512 hasConceptScore W3134966512C49105822 @default.
• W3134966512 hasConceptScore W3134966512C54355233 @default.
• W3134966512 hasConceptScore W3134966512C552990157 @default.
• W3134966512 hasConceptScore W3134966512C71924100 @default.
• W3134966512 hasConceptScore W3134966512C86803240 @default.
• W3134966512 hasFunder F4320319985 @default.
• W3134966512 hasIssue "8" @default.
• W3134966512 hasLocation W31349665121 @default.
• W3134966512 hasLocation W31349665122 @default.
• W3134966512 hasLocation W31349665123 @default.
• W3134966512 hasOpenAccess W3134966512 @default.
• W3134966512 hasPrimaryLocation W31349665121 @default.

• next