FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134979737> ?p ?o ?g. }

• W3134979737 endingPage "4316" @default.
• W3134979737 startingPage "4305" @default.
• W3134979737 abstract "A robust monoclonal antibody (mAb) bioprocess requires physiological parameters such as temperature, pH, or dissolved oxygen to be well-controlled as even small variations in them could potentially impact the final product quality. For instance, pH substantially affects N-glycosylation, protein aggregation, and charge variant profiles, as well as mAb productivity. However, relatively less is known about how pH jointly influences product quality and titer. In this study, we investigated the effect of pH on culture performance, product titer, and quality profiles by applying longitudinal multi-omics profiling, including transcriptomics, proteomics, metabolomics, and glycomics, at three different culture pH set points. The subsequent systematic analysis of multi-omics data showed that pH set points differentially regulated various intracellular pathways including intracellular vesicular trafficking, cell cycle, and apoptosis, thereby resulting in differences in specific productivity, product titer, and quality profiles. In addition, a time-dependent variation in mAb N-glycosylation profiles, independent of pH, was identified to be mainly due to the accumulation of mAb proteins in the endoplasmic reticulum disrupting cellular homeostasis over culture time. Overall, this multi-omics-based study provides an in-depth understanding of the intracellular processes in mAb-producing CHO cell line under varied pH conditions, and could serve as a baseline for enabling the quality optimization and control of mAb production." @default.
• W3134979737 created "2021-03-15" @default.
• W3134979737 creator A5004377544 @default.
• W3134979737 creator A5009629810 @default.
• W3134979737 creator A5010989924 @default.
• W3134979737 creator A5011206845 @default.
• W3134979737 creator A5013536564 @default.
• W3134979737 creator A5019800530 @default.
• W3134979737 creator A5021671918 @default.
• W3134979737 creator A5029580669 @default.
• W3134979737 creator A5040003348 @default.
• W3134979737 creator A5051053743 @default.
• W3134979737 creator A5058916244 @default.
• W3134979737 creator A5062286925 @default.
• W3134979737 creator A5063831919 @default.
• W3134979737 creator A5068320129 @default.
• W3134979737 creator A5070616342 @default.
• W3134979737 creator A5071754158 @default.
• W3134979737 creator A5074628946 @default.
• W3134979737 creator A5082580296 @default.
• W3134979737 creator A5090895640 @default.
• W3134979737 date "2021-07-31" @default.
• W3134979737 modified "2023-10-16" @default.
• W3134979737 title "Multi‐omics profiling of a CHO cell culture system unravels the effect of culture pH on cell growth, antibody titer, and product quality" @default.
• W3134979737 cites W1483007604 @default.
• W3134979737 cites W1972260684 @default.
• W3134979737 cites W1990260057 @default.
• W3134979737 cites W1993424762 @default.
• W3134979737 cites W2028489320 @default.
• W3134979737 cites W2043275103 @default.
• W3134979737 cites W2047774085 @default.
• W3134979737 cites W2078317394 @default.
• W3134979737 cites W2083278580 @default.
• W3134979737 cites W2086873619 @default.
• W3134979737 cites W2086951076 @default.
• W3134979737 cites W2088713628 @default.
• W3134979737 cites W2109662957 @default.
• W3134979737 cites W2130274346 @default.
• W3134979737 cites W2130410032 @default.
• W3134979737 cites W2137354517 @default.
• W3134979737 cites W2137769328 @default.
• W3134979737 cites W2143604769 @default.
• W3134979737 cites W2155819450 @default.
• W3134979737 cites W2163020233 @default.
• W3134979737 cites W2170474571 @default.
• W3134979737 cites W2183107372 @default.
• W3134979737 cites W2298501561 @default.
• W3134979737 cites W2401202998 @default.
• W3134979737 cites W2488318721 @default.
• W3134979737 cites W2508555854 @default.
• W3134979737 cites W2509507123 @default.
• W3134979737 cites W2587355757 @default.
• W3134979737 cites W2597879482 @default.
• W3134979737 cites W2604814997 @default.
• W3134979737 cites W2620082692 @default.
• W3134979737 cites W2735303773 @default.
• W3134979737 cites W2777682310 @default.
• W3134979737 cites W2794768537 @default.
• W3134979737 cites W2885283829 @default.
• W3134979737 cites W2890200200 @default.
• W3134979737 cites W2891499887 @default.
• W3134979737 cites W2897506399 @default.
• W3134979737 cites W2907985438 @default.
• W3134979737 cites W2947330797 @default.
• W3134979737 cites W2947424009 @default.
• W3134979737 cites W2952462326 @default.
• W3134979737 cites W2953352762 @default.
• W3134979737 doi "https://doi.org/10.1002/bit.27899" @default.
• W3134979737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34289087" @default.
• W3134979737 hasPublicationYear "2021" @default.
• W3134979737 type Work @default.
• W3134979737 sameAs 3134979737 @default.
• W3134979737 citedByCount "8" @default.
• W3134979737 countsByYear W31349797372022 @default.
• W3134979737 countsByYear W31349797372023 @default.
• W3134979737 crossrefType "journal-article" @default.
• W3134979737 hasAuthorship W3134979737A5004377544 @default.
• W3134979737 hasAuthorship W3134979737A5009629810 @default.
• W3134979737 hasAuthorship W3134979737A5010989924 @default.
• W3134979737 hasAuthorship W3134979737A5011206845 @default.
• W3134979737 hasAuthorship W3134979737A5013536564 @default.
• W3134979737 hasAuthorship W3134979737A5019800530 @default.
• W3134979737 hasAuthorship W3134979737A5021671918 @default.
• W3134979737 hasAuthorship W3134979737A5029580669 @default.
• W3134979737 hasAuthorship W3134979737A5040003348 @default.
• W3134979737 hasAuthorship W3134979737A5051053743 @default.
• W3134979737 hasAuthorship W3134979737A5058916244 @default.
• W3134979737 hasAuthorship W3134979737A5062286925 @default.
• W3134979737 hasAuthorship W3134979737A5063831919 @default.
• W3134979737 hasAuthorship W3134979737A5068320129 @default.
• W3134979737 hasAuthorship W3134979737A5070616342 @default.
• W3134979737 hasAuthorship W3134979737A5071754158 @default.
• W3134979737 hasAuthorship W3134979737A5074628946 @default.
• W3134979737 hasAuthorship W3134979737A5082580296 @default.
• W3134979737 hasAuthorship W3134979737A5090895640 @default.
• W3134979737 hasBestOaLocation W31349797372 @default.
• W3134979737 hasConcept C104317684 @default.
• W3134979737 hasConcept C151730666 @default.

• next