Matches in SemOpenAlex for { <https://semopenalex.org/work/W3135162893> ?p ?o ?g. }
- W3135162893 endingPage "1080" @default.
- W3135162893 startingPage "1063" @default.
- W3135162893 abstract "To harness the full potential of human pluripotent stem cells (hPSCs) we combined instrumented stirred tank bioreactor (STBR) technology with the power of in silico process modeling to overcome substantial, hPSC-specific hurdles toward their mass production. Perfused suspension culture (3D) of matrix-free hPSC aggregates in STBRs was applied to identify and control process-limiting parameters including pH, dissolved oxygen, glucose and lactate levels, and the obviation of osmolality peaks provoked by high density culture. Media supplements promoted single cell-based process inoculation and hydrodynamic aggregate size control. Wet lab-derived process characteristics enabled predictive in silico modeling as a new rational for hPSC cultivation. Consequently, hPSC line-independent maintenance of exponential cell proliferation was achieved. The strategy yielded 70-fold cell expansion in 7 days achieving an unmatched density of 35 × 106 cells/mL equivalent to 5.25 billion hPSC in 150 mL scale while pluripotency, differentiation potential, and karyotype stability was maintained. In parallel, media requirements were reduced by 75% demonstrating the outstanding increase in efficiency. Minimal input to our in silico model accurately predicts all main process parameters; combined with calculation-controlled hPSC aggregation kinetics, linear process upscaling is also enabled and demonstrated for up to 500 mL scale in an independent bioreactor system. Thus, by merging applied stem cell research with recent knowhow from industrial cell fermentation, a new level of hPSC bioprocessing is revealed fueling their automated production for industrial and therapeutic applications." @default.
- W3135162893 created "2021-03-15" @default.
- W3135162893 creator A5007211688 @default.
- W3135162893 creator A5015353416 @default.
- W3135162893 creator A5021011392 @default.
- W3135162893 creator A5022074063 @default.
- W3135162893 creator A5025880687 @default.
- W3135162893 creator A5030928136 @default.
- W3135162893 creator A5043035962 @default.
- W3135162893 creator A5047215753 @default.
- W3135162893 creator A5051547649 @default.
- W3135162893 creator A5060753871 @default.
- W3135162893 creator A5060776063 @default.
- W3135162893 creator A5067433541 @default.
- W3135162893 creator A5075108571 @default.
- W3135162893 creator A5076308958 @default.
- W3135162893 creator A5079825528 @default.
- W3135162893 creator A5081495966 @default.
- W3135162893 date "2021-03-04" @default.
- W3135162893 modified "2023-10-16" @default.
- W3135162893 title "High Density Bioprocessing of Human Pluripotent Stem Cells by Metabolic Control and in Silico Modeling" @default.
- W3135162893 cites W1522139880 @default.
- W3135162893 cites W1561765789 @default.
- W3135162893 cites W1609890651 @default.
- W3135162893 cites W1841567071 @default.
- W3135162893 cites W1906645663 @default.
- W3135162893 cites W1964324925 @default.
- W3135162893 cites W1972829016 @default.
- W3135162893 cites W1974786321 @default.
- W3135162893 cites W1977731315 @default.
- W3135162893 cites W1988865085 @default.
- W3135162893 cites W1989832576 @default.
- W3135162893 cites W1992816164 @default.
- W3135162893 cites W2004051083 @default.
- W3135162893 cites W2004518428 @default.
- W3135162893 cites W2004863952 @default.
- W3135162893 cites W2026050064 @default.
- W3135162893 cites W2026737836 @default.
- W3135162893 cites W2028401005 @default.
- W3135162893 cites W2030775222 @default.
- W3135162893 cites W2040067847 @default.
- W3135162893 cites W2040610472 @default.
- W3135162893 cites W2043221475 @default.
- W3135162893 cites W2060315853 @default.
- W3135162893 cites W2069019884 @default.
- W3135162893 cites W2071444953 @default.
- W3135162893 cites W2076289226 @default.
- W3135162893 cites W2081635461 @default.
- W3135162893 cites W2083967847 @default.
- W3135162893 cites W2089271595 @default.
- W3135162893 cites W2109570573 @default.
- W3135162893 cites W2133575765 @default.
- W3135162893 cites W2143258723 @default.
- W3135162893 cites W2170409651 @default.
- W3135162893 cites W2174275312 @default.
- W3135162893 cites W2182674334 @default.
- W3135162893 cites W2259641960 @default.
- W3135162893 cites W2264267198 @default.
- W3135162893 cites W2281224598 @default.
- W3135162893 cites W2340402631 @default.
- W3135162893 cites W2408822935 @default.
- W3135162893 cites W2463534616 @default.
- W3135162893 cites W2491616054 @default.
- W3135162893 cites W2516304141 @default.
- W3135162893 cites W2535579130 @default.
- W3135162893 cites W2566993351 @default.
- W3135162893 cites W2567672025 @default.
- W3135162893 cites W2580933284 @default.
- W3135162893 cites W2600885036 @default.
- W3135162893 cites W2609543204 @default.
- W3135162893 cites W2612493242 @default.
- W3135162893 cites W2613669273 @default.
- W3135162893 cites W2614283331 @default.
- W3135162893 cites W2621387568 @default.
- W3135162893 cites W2740874620 @default.
- W3135162893 cites W2756568237 @default.
- W3135162893 cites W2762440986 @default.
- W3135162893 cites W2792576987 @default.
- W3135162893 cites W2794136421 @default.
- W3135162893 cites W2801205060 @default.
- W3135162893 cites W2802183709 @default.
- W3135162893 cites W2885389744 @default.
- W3135162893 cites W2888442091 @default.
- W3135162893 cites W2898632009 @default.
- W3135162893 cites W2902342830 @default.
- W3135162893 cites W2907128322 @default.
- W3135162893 cites W2911713086 @default.
- W3135162893 cites W2940705263 @default.
- W3135162893 cites W2945027985 @default.
- W3135162893 cites W2949361292 @default.
- W3135162893 cites W2963782603 @default.
- W3135162893 cites W2969759844 @default.
- W3135162893 cites W2971523738 @default.
- W3135162893 cites W2975155102 @default.
- W3135162893 cites W2994153296 @default.
- W3135162893 cites W3135162893 @default.
- W3135162893 cites W4245870441 @default.
- W3135162893 cites W113427182 @default.