Matches in SemOpenAlex for { <https://semopenalex.org/work/W3135439632> ?p ?o ?g. }
- W3135439632 endingPage "4129" @default.
- W3135439632 startingPage "4122" @default.
- W3135439632 abstract "Chiral amines are a common feature of many active pharmaceutical ingredients. The synthesis of very small chiral amines is particularly challenging, even via biocatalytic routes, as the level of discrimination between similarly sized R-groups must be exceptional, yet their synthesis creates attractive building blocks that may then be used to prepare diverse compounds in further steps. Herein, the synthesis of one of the smallest chiral amines, 2-aminobutane, using transaminases, is being investigated. After screening a panel of mainly wild-type transaminases, two candidates were identified: an (S)-selective transaminase from Halomonas elongata (HEwT) and a precommercial (R)-selective transaminase from Johnson Matthey (*RTA-X43). Notably, a single strategic point mutation enhanced the enantioselectivity of HEwT from 45 to >99.5% ee. By covalently immobilizing these candidates, both enantiomers of 2-aminobutane were synthesized on a multigram scale, and the feasibility of isolation by distillation without the need for any solvents other than water was demonstrated. The atom economy of the process was calculated to be 56% and the E-factors (including waste generated during enzyme expression and immobilization) were 55 and 48 for the synthesis of (R)-2-aminobutane and (S)-2-aminobutane, respectively." @default.
- W3135439632 created "2021-03-15" @default.
- W3135439632 creator A5003578714 @default.
- W3135439632 creator A5025548733 @default.
- W3135439632 creator A5025739763 @default.
- W3135439632 date "2021-03-05" @default.
- W3135439632 modified "2023-10-09" @default.
- W3135439632 title "Enantio-Complementary Continuous-Flow Synthesis of 2-Aminobutane Using Covalently Immobilized Transaminases" @default.
- W3135439632 cites W1973227340 @default.
- W3135439632 cites W1974749879 @default.
- W3135439632 cites W1986056153 @default.
- W3135439632 cites W2024851405 @default.
- W3135439632 cites W2036834383 @default.
- W3135439632 cites W2040851590 @default.
- W3135439632 cites W2041571635 @default.
- W3135439632 cites W2051449766 @default.
- W3135439632 cites W2062587578 @default.
- W3135439632 cites W2077666873 @default.
- W3135439632 cites W2079848175 @default.
- W3135439632 cites W2101849387 @default.
- W3135439632 cites W2112306226 @default.
- W3135439632 cites W2134967712 @default.
- W3135439632 cites W2156884309 @default.
- W3135439632 cites W2291624720 @default.
- W3135439632 cites W2332007112 @default.
- W3135439632 cites W2493886501 @default.
- W3135439632 cites W2496645452 @default.
- W3135439632 cites W2521399459 @default.
- W3135439632 cites W2524638452 @default.
- W3135439632 cites W2580316284 @default.
- W3135439632 cites W2606664565 @default.
- W3135439632 cites W2790025101 @default.
- W3135439632 cites W2808176740 @default.
- W3135439632 cites W2899509746 @default.
- W3135439632 cites W2902996841 @default.
- W3135439632 cites W2911428326 @default.
- W3135439632 cites W2919948904 @default.
- W3135439632 cites W2944045445 @default.
- W3135439632 cites W2944582664 @default.
- W3135439632 cites W3034581495 @default.
- W3135439632 cites W3036931001 @default.
- W3135439632 cites W945384332 @default.
- W3135439632 doi "https://doi.org/10.1021/acssuschemeng.0c09075" @default.
- W3135439632 hasPublicationYear "2021" @default.
- W3135439632 type Work @default.
- W3135439632 sameAs 3135439632 @default.
- W3135439632 citedByCount "10" @default.
- W3135439632 countsByYear W31354396322021 @default.
- W3135439632 countsByYear W31354396322022 @default.
- W3135439632 countsByYear W31354396322023 @default.
- W3135439632 crossrefType "journal-article" @default.
- W3135439632 hasAuthorship W3135439632A5003578714 @default.
- W3135439632 hasAuthorship W3135439632A5025548733 @default.
- W3135439632 hasAuthorship W3135439632A5025739763 @default.
- W3135439632 hasBestOaLocation W31354396322 @default.
- W3135439632 hasConcept C104317684 @default.
- W3135439632 hasConcept C146686406 @default.
- W3135439632 hasConcept C161790260 @default.
- W3135439632 hasConcept C178790620 @default.
- W3135439632 hasConcept C180577832 @default.
- W3135439632 hasConcept C181199279 @default.
- W3135439632 hasConcept C185592680 @default.
- W3135439632 hasConcept C191503008 @default.
- W3135439632 hasConcept C21951064 @default.
- W3135439632 hasConcept C2776034031 @default.
- W3135439632 hasConcept C2776877979 @default.
- W3135439632 hasConcept C3141732 @default.
- W3135439632 hasConcept C42062724 @default.
- W3135439632 hasConcept C486523 @default.
- W3135439632 hasConcept C55493867 @default.
- W3135439632 hasConcept C65024703 @default.
- W3135439632 hasConcept C71240020 @default.
- W3135439632 hasConceptScore W3135439632C104317684 @default.
- W3135439632 hasConceptScore W3135439632C146686406 @default.
- W3135439632 hasConceptScore W3135439632C161790260 @default.
- W3135439632 hasConceptScore W3135439632C178790620 @default.
- W3135439632 hasConceptScore W3135439632C180577832 @default.
- W3135439632 hasConceptScore W3135439632C181199279 @default.
- W3135439632 hasConceptScore W3135439632C185592680 @default.
- W3135439632 hasConceptScore W3135439632C191503008 @default.
- W3135439632 hasConceptScore W3135439632C21951064 @default.
- W3135439632 hasConceptScore W3135439632C2776034031 @default.
- W3135439632 hasConceptScore W3135439632C2776877979 @default.
- W3135439632 hasConceptScore W3135439632C3141732 @default.
- W3135439632 hasConceptScore W3135439632C42062724 @default.
- W3135439632 hasConceptScore W3135439632C486523 @default.
- W3135439632 hasConceptScore W3135439632C55493867 @default.
- W3135439632 hasConceptScore W3135439632C65024703 @default.
- W3135439632 hasConceptScore W3135439632C71240020 @default.
- W3135439632 hasFunder F4320334629 @default.
- W3135439632 hasIssue "11" @default.
- W3135439632 hasLocation W31354396321 @default.
- W3135439632 hasLocation W31354396322 @default.
- W3135439632 hasLocation W31354396323 @default.
- W3135439632 hasLocation W31354396324 @default.
- W3135439632 hasOpenAccess W3135439632 @default.
- W3135439632 hasPrimaryLocation W31354396321 @default.
- W3135439632 hasRelatedWork W1956888186 @default.