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- W3135508725 abstract "In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: (i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and (ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. The strategy of combining UCNPs, magnetic microparticles, and a photoligation reaction provides new insight into low-cost, rapid, and ultra-sensitive detection of oligonucleotide sequences." @default.
- W3135508725 created "2021-03-15" @default.
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- W3135508725 date "2021-08-01" @default.
- W3135508725 modified "2023-10-01" @default.
- W3135508725 title "Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces" @default.
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- W3135508725 doi "https://doi.org/10.1016/j.jcis.2021.02.093" @default.
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