FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3135518472> ?p ?o ?g. }

• W3135518472 endingPage "399" @default.
• W3135518472 startingPage "399" @default.
• W3135518472 abstract "tRNA methyltransferase 5 (Trm5) enzyme is an S-adenosyl methionine (AdoMet)-dependent methyltransferase which methylates the G37 nucleotide at the N1 atom of the tRNA. The free form of Trm5 enzyme has three intrinsically disordered regions, which are highly flexible and lack stable three-dimensional structures. These regions gain ordered structures upon the complex formation with tRNA, also called disorder-to-order transition (DOT) regions. In this study, we performed molecular dynamics (MD) simulations of archaeal Trm5 in free and complex forms and observed that the DOT residues are highly flexible in free proteins and become stable in complex structures. The energetic contributions show that DOT residues are important for stabilising the complex. The DOT1 and DOT2 are mainly observed to be important for stabilising the complex, while DOT3 is present near the active site to coordinate the interactions between methyl-donating ligands and G37 nucleotides. In addition, mutational studies on the Trm5 complex showed that the wild type is more stable than the G37A tRNA mutant complex. The loss of productive interactions upon G37A mutation drives the AdoMet ligand away from the 37th nucleotide, and Arg145 in DOT3 plays a crucial role in stabilising the ligand, as well as the G37 nucleotide, in the wild-type complex. Further, the overall energetic contribution calculated using MMPBSA corroborates that the wild-type complex has a better affinity between Trm5 and tRNA. Overall, our study reveals that targeting DOT regions for binding could improve the inhibition of Trm5." @default.
• W3135518472 created "2021-03-15" @default.
• W3135518472 creator A5012378866 @default.
• W3135518472 creator A5013544358 @default.
• W3135518472 creator A5028328692 @default.
• W3135518472 creator A5046879365 @default.
• W3135518472 date "2021-03-11" @default.
• W3135518472 modified "2023-09-23" @default.
• W3135518472 title "Deciphering the Role of Residues Involved in Disorder-To-Order Transition Regions in Archaeal tRNA Methyltransferase 5" @default.
• W3135518472 cites W1607931568 @default.
• W3135518472 cites W1753285579 @default.
• W3135518472 cites W1972433022 @default.
• W3135518472 cites W1976499671 @default.
• W3135518472 cites W1999613945 @default.
• W3135518472 cites W2026004144 @default.
• W3135518472 cites W2029318943 @default.
• W3135518472 cites W2029667189 @default.
• W3135518472 cites W2037555428 @default.
• W3135518472 cites W2052485230 @default.
• W3135518472 cites W2053238521 @default.
• W3135518472 cites W2058680552 @default.
• W3135518472 cites W2083507245 @default.
• W3135518472 cites W2084788365 @default.
• W3135518472 cites W2095719702 @default.
• W3135518472 cites W2114857744 @default.
• W3135518472 cites W2132629607 @default.
• W3135518472 cites W2133666260 @default.
• W3135518472 cites W2135841502 @default.
• W3135518472 cites W2144288821 @default.
• W3135518472 cites W2154208264 @default.
• W3135518472 cites W2167066426 @default.
• W3135518472 cites W2291981194 @default.
• W3135518472 cites W2332712348 @default.
• W3135518472 cites W2601992375 @default.
• W3135518472 cites W2898364362 @default.
• W3135518472 cites W2912126500 @default.
• W3135518472 cites W2951087753 @default.
• W3135518472 cites W2954342019 @default.
• W3135518472 cites W2969448013 @default.
• W3135518472 cites W2989581388 @default.
• W3135518472 cites W3004647566 @default.
• W3135518472 cites W3005851282 @default.
• W3135518472 cites W3018137979 @default.
• W3135518472 doi "https://doi.org/10.3390/genes12030399" @default.
• W3135518472 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8000304" @default.
• W3135518472 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33799704" @default.
• W3135518472 hasPublicationYear "2021" @default.
• W3135518472 type Work @default.
• W3135518472 sameAs 3135518472 @default.
• W3135518472 citedByCount "0" @default.
• W3135518472 crossrefType "journal-article" @default.
• W3135518472 hasAuthorship W3135518472A5012378866 @default.
• W3135518472 hasAuthorship W3135518472A5013544358 @default.
• W3135518472 hasAuthorship W3135518472A5028328692 @default.
• W3135518472 hasAuthorship W3135518472A5046879365 @default.
• W3135518472 hasBestOaLocation W31355184721 @default.
• W3135518472 hasConcept C104317684 @default.
• W3135518472 hasConcept C116569031 @default.
• W3135518472 hasConcept C143065580 @default.
• W3135518472 hasConcept C153957851 @default.
• W3135518472 hasConcept C170493617 @default.
• W3135518472 hasConcept C181199279 @default.
• W3135518472 hasConcept C194232998 @default.
• W3135518472 hasConcept C33288867 @default.
• W3135518472 hasConcept C41183919 @default.
• W3135518472 hasConcept C512185932 @default.
• W3135518472 hasConcept C552990157 @default.
• W3135518472 hasConcept C55493867 @default.
• W3135518472 hasConcept C67705224 @default.
• W3135518472 hasConcept C86803240 @default.
• W3135518472 hasConcept C91965660 @default.
• W3135518472 hasConceptScore W3135518472C104317684 @default.
• W3135518472 hasConceptScore W3135518472C116569031 @default.
• W3135518472 hasConceptScore W3135518472C143065580 @default.
• W3135518472 hasConceptScore W3135518472C153957851 @default.
• W3135518472 hasConceptScore W3135518472C170493617 @default.
• W3135518472 hasConceptScore W3135518472C181199279 @default.
• W3135518472 hasConceptScore W3135518472C194232998 @default.
• W3135518472 hasConceptScore W3135518472C33288867 @default.
• W3135518472 hasConceptScore W3135518472C41183919 @default.
• W3135518472 hasConceptScore W3135518472C512185932 @default.
• W3135518472 hasConceptScore W3135518472C552990157 @default.
• W3135518472 hasConceptScore W3135518472C55493867 @default.
• W3135518472 hasConceptScore W3135518472C67705224 @default.
• W3135518472 hasConceptScore W3135518472C86803240 @default.
• W3135518472 hasConceptScore W3135518472C91965660 @default.
• W3135518472 hasFunder F4320320721 @default.
• W3135518472 hasIssue "3" @default.
• W3135518472 hasLocation W31355184721 @default.
• W3135518472 hasLocation W31355184722 @default.
• W3135518472 hasLocation W31355184723 @default.
• W3135518472 hasOpenAccess W3135518472 @default.
• W3135518472 hasPrimaryLocation W31355184721 @default.
• W3135518472 hasRelatedWork W1532183086 @default.
• W3135518472 hasRelatedWork W1533751437 @default.
• W3135518472 hasRelatedWork W1788095238 @default.
• W3135518472 hasRelatedWork W1968172905 @default.
• W3135518472 hasRelatedWork W1974967661 @default.

• next