FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3135566779> ?p ?o ?g. }

• W3135566779 abstract "Abstract Aminobacter sp. MSH1 (CIP 110285) can use the pesticide dichlobenil and its transformation product, the recalcitrant groundwater micropollutant, 2,6-dichlorobenzamide (BAM) as sole source of carbon, nitrogen, and energy. The concentration of BAM in groundwater often exceeds the threshold limit for drinking water, resulting in the use of additional treatment in drinking water treatment plants (DWTPs) or closure of the affected abstraction wells. Biological treatment with MSH1 is considered a potential sustainable alternative to remediate BAM-contamination in drinking water production. Combining Illumina and Nanopore sequencing, we here present the complete genome of MSH1, which was determined independently in two different laboratories. Unexpectedly, divergences were observed between the two genomes, i.e. one of them lacked four plasmids compared to the other. Besides the circular chromosome and the two previously described plasmids involved in BAM catabolism pBAM1 (41 kb) and pBAM2 (54 kb), we observe that the genome of MSH1 contains two megaplasmids pUSP1 (367 kb) and pUSP2 (366 kb) and three smaller plasmids pUSP3 (97 kb), pUSP4 (64 kb), and pUSP5 (32 kb). The MSH1 substrain from KU Leuven showed a reduced genome lacking plasmids pUSP2 and the three smaller plasmids and was designated substrain MK1, whereas the variant with all plasmids was designated as substrain DK1. Results of a plasmid stability experiment, indicate that strain MSH1 may have a polyploid chromosome when growing in R2B medium with more chromosomes than plasmids per cell. Based on phylogenetic analyses, strain MSH1 is reassigned as Aminobacter niigataensis MSH1. Importance The complete genomes of the two MSH1 substrains, DK1 and MK1, provide further insight into this already well-studied organism with bioremediation potential. The varying plasmid contents in the two substrains suggest that some of the plasmids are unstable, although this is not supported by the herein described plasmid stability experiment. Instead, results suggest that MSH1 is polyploid with respect to its chromosome, at least under some growth conditions. As the essential BAM-degradation genes are found on some of these plasmids, stable inheritance is essential for continuous removal of BAM. Finally, Aminobacter sp. MSH1 is reassigned as Aminobacter niigataensis MSH1, based on phylogenetic evidence." @default.
• W3135566779 created "2021-03-15" @default.
• W3135566779 creator A5011831685 @default.
• W3135566779 creator A5017631683 @default.
• W3135566779 creator A5020660164 @default.
• W3135566779 creator A5026085887 @default.
• W3135566779 creator A5052218386 @default.
• W3135566779 creator A5053249883 @default.
• W3135566779 creator A5054410221 @default.
• W3135566779 creator A5056568447 @default.
• W3135566779 creator A5061486081 @default.
• W3135566779 creator A5066840503 @default.
• W3135566779 creator A5084654727 @default.
• W3135566779 date "2021-02-27" @default.
• W3135566779 modified "2023-09-27" @default.
• W3135566779 title "Analyses of the complete genome sequence of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of (un)stable plasmids" @default.
• W3135566779 cites W1587738852 @default.
• W3135566779 cites W1992301911 @default.
• W3135566779 cites W2004548026 @default.
• W3135566779 cites W2007857790 @default.
• W3135566779 cites W2013737863 @default.
• W3135566779 cites W2019014466 @default.
• W3135566779 cites W2020689286 @default.
• W3135566779 cites W2052212080 @default.
• W3135566779 cites W2062249432 @default.
• W3135566779 cites W2063700392 @default.
• W3135566779 cites W2076597748 @default.
• W3135566779 cites W2078651213 @default.
• W3135566779 cites W2089241375 @default.
• W3135566779 cites W2089594628 @default.
• W3135566779 cites W2096093282 @default.
• W3135566779 cites W2102619694 @default.
• W3135566779 cites W2103441770 @default.
• W3135566779 cites W2106814707 @default.
• W3135566779 cites W2108234281 @default.
• W3135566779 cites W2116199374 @default.
• W3135566779 cites W2125119899 @default.
• W3135566779 cites W2127322768 @default.
• W3135566779 cites W2131271579 @default.
• W3135566779 cites W2143898755 @default.
• W3135566779 cites W2152770371 @default.
• W3135566779 cites W2158804744 @default.
• W3135566779 cites W216106564 @default.
• W3135566779 cites W2164426273 @default.
• W3135566779 cites W2263582186 @default.
• W3135566779 cites W2496354512 @default.
• W3135566779 cites W2509130909 @default.
• W3135566779 cites W2691973205 @default.
• W3135566779 cites W2765281411 @default.
• W3135566779 cites W2768079869 @default.
• W3135566779 cites W2799524357 @default.
• W3135566779 cites W2851578659 @default.
• W3135566779 cites W2884772290 @default.
• W3135566779 cites W2946204549 @default.
• W3135566779 cites W2949841808 @default.
• W3135566779 cites W2951254987 @default.
• W3135566779 cites W2952167528 @default.
• W3135566779 cites W2965008468 @default.
• W3135566779 cites W3033347221 @default.
• W3135566779 cites W4236875018 @default.
• W3135566779 cites W641877579 @default.
• W3135566779 doi "https://doi.org/10.1101/2021.02.25.432982" @default.
• W3135566779 hasPublicationYear "2021" @default.
• W3135566779 type Work @default.
• W3135566779 sameAs 3135566779 @default.
• W3135566779 citedByCount "0" @default.
• W3135566779 crossrefType "posted-content" @default.
• W3135566779 hasAuthorship W3135566779A5011831685 @default.
• W3135566779 hasAuthorship W3135566779A5017631683 @default.
• W3135566779 hasAuthorship W3135566779A5020660164 @default.
• W3135566779 hasAuthorship W3135566779A5026085887 @default.
• W3135566779 hasAuthorship W3135566779A5052218386 @default.
• W3135566779 hasAuthorship W3135566779A5053249883 @default.
• W3135566779 hasAuthorship W3135566779A5054410221 @default.
• W3135566779 hasAuthorship W3135566779A5056568447 @default.
• W3135566779 hasAuthorship W3135566779A5061486081 @default.
• W3135566779 hasAuthorship W3135566779A5066840503 @default.
• W3135566779 hasAuthorship W3135566779A5084654727 @default.
• W3135566779 hasBestOaLocation W31355667791 @default.
• W3135566779 hasConcept C104317684 @default.
• W3135566779 hasConcept C141231307 @default.
• W3135566779 hasConcept C185592680 @default.
• W3135566779 hasConcept C22744801 @default.
• W3135566779 hasConcept C30481170 @default.
• W3135566779 hasConcept C54355233 @default.
• W3135566779 hasConcept C86803240 @default.
• W3135566779 hasConceptScore W3135566779C104317684 @default.
• W3135566779 hasConceptScore W3135566779C141231307 @default.
• W3135566779 hasConceptScore W3135566779C185592680 @default.
• W3135566779 hasConceptScore W3135566779C22744801 @default.
• W3135566779 hasConceptScore W3135566779C30481170 @default.
• W3135566779 hasConceptScore W3135566779C54355233 @default.
• W3135566779 hasConceptScore W3135566779C86803240 @default.
• W3135566779 hasLocation W31355667791 @default.
• W3135566779 hasLocation W31355667792 @default.
• W3135566779 hasOpenAccess W3135566779 @default.
• W3135566779 hasPrimaryLocation W31355667791 @default.
• W3135566779 hasRelatedWork W2013690240 @default.
• W3135566779 hasRelatedWork W2031436818 @default.
• W3135566779 hasRelatedWork W2050897266 @default.

• next