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• W3136111759 endingPage "129633" @default.
• W3136111759 startingPage "129633" @default.
• W3136111759 abstract "• A novel Au-Ag NCs-based ratiometric fluorescence ELISA for zearalenone was developed. • The detection mechanism was through inner filter effect induced Au-Ag NCs quenching. • The limit of detection was approximately 6.6-fold lower than conventional ELISA. • The method for real corn samples showed reliability and high correlation with ELISA. In the presented study, a horseradish peroxidase (HRP)-mediated ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) for zearalenone (ZEN) was reported based on fluorescence quenching of gold-silver bimetallic nanoclusters (Au-Ag NCs). HRP-antibody was used as a bridge in this immunoassay, linking the ratiometric fluorescence signal to the ZEN concentration. HRP catalyzed the oxidization of o-phenylenediamine in the presence of H 2 O 2 , leading to the formation of 2,3-diaminophenazine, which not only delivered a new peak at 580 nm but also quenched Au-Ag NCs fluorescence at 690 nm. Under optimal conditions, the detection limit for the proposed ELISA was 0.017 ng/mL, which was approximately 6.6-fold lower than conventional ELISA. Moreover, analytical performances were evaluated fully including specificity, accuracy, precision, and practicability, and showed that this method provides a potential platform for sensitive and reliable detection of ZEN." @default.
• W3136111759 created "2021-03-29" @default.
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• W3136111759 date "2021-09-01" @default.
• W3136111759 modified "2023-09-26" @default.
• W3136111759 title "Novel fluorescence immunoassay for the detection of zearalenone using HRP-mediated fluorescence quenching of gold-silver bimetallic nanoclusters" @default.
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• W3136111759 doi "https://doi.org/10.1016/j.foodchem.2021.129633" @default.
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