Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136173882> ?p ?o ?g. }
- W3136173882 endingPage "796" @default.
- W3136173882 startingPage "784" @default.
- W3136173882 abstract "The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs) migrate through the bloodstream and reach the gonadal anlage, where they develop into mature germ cells. Here, we explored the use of PGCs to produce transgenic offspring in the zebra finch, which is a major animal model for sexual brain differentiation, vocal learning, and vocal communication. Zebra finch PGCs (zfPGCs) obtained from embryonic blood significantly proliferated when cultured in an optimized culture medium and conserved the expression of germ and stem cell markers. Transduction of cultured zfPGCs with lentiviral vectors was highly efficient, leading to strong expression of the enhanced green fluorescent protein. Transduced zfPGCs were injected into the host embryo and transgenic songbirds were successfully generated." @default.
- W3136173882 created "2021-03-29" @default.
- W3136173882 creator A5021637241 @default.
- W3136173882 creator A5031422181 @default.
- W3136173882 creator A5045826233 @default.
- W3136173882 creator A5048401390 @default.
- W3136173882 creator A5058274282 @default.
- W3136173882 creator A5059181714 @default.
- W3136173882 creator A5071705919 @default.
- W3136173882 creator A5091100451 @default.
- W3136173882 date "2021-04-01" @default.
- W3136173882 modified "2023-10-12" @default.
- W3136173882 title "Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds" @default.
- W3136173882 cites W1532285268 @default.
- W3136173882 cites W1543247314 @default.
- W3136173882 cites W1666878639 @default.
- W3136173882 cites W1847753680 @default.
- W3136173882 cites W1990541608 @default.
- W3136173882 cites W1997447798 @default.
- W3136173882 cites W2020920887 @default.
- W3136173882 cites W2021290198 @default.
- W3136173882 cites W2026234065 @default.
- W3136173882 cites W2039751785 @default.
- W3136173882 cites W2048064609 @default.
- W3136173882 cites W2053247560 @default.
- W3136173882 cites W2057987333 @default.
- W3136173882 cites W2119680107 @default.
- W3136173882 cites W2124941761 @default.
- W3136173882 cites W2131271579 @default.
- W3136173882 cites W2131569286 @default.
- W3136173882 cites W2136852632 @default.
- W3136173882 cites W2138207763 @default.
- W3136173882 cites W2140034535 @default.
- W3136173882 cites W2142092610 @default.
- W3136173882 cites W2158071031 @default.
- W3136173882 cites W2161820572 @default.
- W3136173882 cites W2167569042 @default.
- W3136173882 cites W2168502079 @default.
- W3136173882 cites W2169456326 @default.
- W3136173882 cites W2170529568 @default.
- W3136173882 cites W2173835926 @default.
- W3136173882 cites W2179438025 @default.
- W3136173882 cites W2270183241 @default.
- W3136173882 cites W2296631988 @default.
- W3136173882 cites W2315885991 @default.
- W3136173882 cites W2341245738 @default.
- W3136173882 cites W2399463045 @default.
- W3136173882 cites W2416873505 @default.
- W3136173882 cites W2432815617 @default.
- W3136173882 cites W2474091620 @default.
- W3136173882 cites W2571028618 @default.
- W3136173882 cites W2586599127 @default.
- W3136173882 cites W2743956684 @default.
- W3136173882 cites W2793196316 @default.
- W3136173882 cites W2811013894 @default.
- W3136173882 cites W2897141255 @default.
- W3136173882 cites W2917207851 @default.
- W3136173882 cites W2980081995 @default.
- W3136173882 cites W3012643041 @default.
- W3136173882 doi "https://doi.org/10.1016/j.stemcr.2021.02.015" @default.
- W3136173882 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8072032" @default.
- W3136173882 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33740464" @default.
- W3136173882 hasPublicationYear "2021" @default.
- W3136173882 type Work @default.
- W3136173882 sameAs 3136173882 @default.
- W3136173882 citedByCount "10" @default.
- W3136173882 countsByYear W31361738822021 @default.
- W3136173882 countsByYear W31361738822022 @default.
- W3136173882 countsByYear W31361738822023 @default.
- W3136173882 crossrefType "journal-article" @default.
- W3136173882 hasAuthorship W3136173882A5021637241 @default.
- W3136173882 hasAuthorship W3136173882A5031422181 @default.
- W3136173882 hasAuthorship W3136173882A5045826233 @default.
- W3136173882 hasAuthorship W3136173882A5048401390 @default.
- W3136173882 hasAuthorship W3136173882A5058274282 @default.
- W3136173882 hasAuthorship W3136173882A5059181714 @default.
- W3136173882 hasAuthorship W3136173882A5071705919 @default.
- W3136173882 hasAuthorship W3136173882A5091100451 @default.
- W3136173882 hasBestOaLocation W31361738821 @default.
- W3136173882 hasConcept C102230213 @default.
- W3136173882 hasConcept C104317684 @default.
- W3136173882 hasConcept C109825262 @default.
- W3136173882 hasConcept C141231307 @default.
- W3136173882 hasConcept C145103041 @default.
- W3136173882 hasConcept C15152581 @default.
- W3136173882 hasConcept C169760540 @default.
- W3136173882 hasConcept C196843134 @default.
- W3136173882 hasConcept C2779122487 @default.
- W3136173882 hasConcept C28328180 @default.
- W3136173882 hasConcept C2992280869 @default.
- W3136173882 hasConcept C54355233 @default.
- W3136173882 hasConcept C55493867 @default.
- W3136173882 hasConcept C86803240 @default.
- W3136173882 hasConcept C95444343 @default.
- W3136173882 hasConceptScore W3136173882C102230213 @default.
- W3136173882 hasConceptScore W3136173882C104317684 @default.
- W3136173882 hasConceptScore W3136173882C109825262 @default.
- W3136173882 hasConceptScore W3136173882C141231307 @default.