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- W3136243045 abstract "Chemogenetic property of mercuric ion (Hg2+) was investigated as a specific hypercalcemia actuator in the neuronal spinal cord cell manipulation by Zeta-based potentiometric bio-sensing analysis via introducing a novel array-based Hg2+ bio-sensor. For this purpose, the array of a two-electrode system including Ag/AgCl (sat'd Cl−) as reference electrode and a paste nano-composite as the indicator electrode was utilized. The indicator electrode was made of activated multi-walled carbon nanotubes as conductive support, a grounded slice of sheep's spinal cord as natural neuron stem cells (ionophore), and oxalate ion as both the dispersed phase and cationic site. Under optimum conditions by one-at-a-time method, a two-linear range between 1.3 × 10−4- 6.5 × 10−12 and 2.7 × 10−14- 1.4 × 10−21 mol L−1 with correlation coefficients (R2) of 0.96 and 0.99, respectively, and response time (t90) of maximum 5.0 min were approximated. The percentages of relative standard deviation were estimated to be 4.05 (repeatability, n = 10) and 6.14 (reproducibility, n = 12). The detection limit was estimated to be sub 5.3 × 10−22 mol L−1 based on the X̄b+3Sb. The reliability of this phenomenon was evidenced by different analytical techniques. The Zeta-based electrical response was therefore attributed to highly Ca2+ pumping from the stem cells ionic channel gates as the proposed mechanistic behavior of the spinal cord. Actuating (triggering) the stem cells by Hg2+ consequently led to generate significant Zeta potential as the proposed mechanism. The results pointed to the potentiometric responsibility of a protein with gram molecular weight of 66.2 ± 0.3 KCU in the stem cell matrix as a specific hypercalcemia actuator." @default.
- W3136243045 created "2021-03-29" @default.
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- W3136243045 date "2021-06-01" @default.
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- W3136243045 title "Chemogenetic biocompatibly of mercury as specific hypercalcemia actuator in neuronal spinal cord cell manipulation: Zeta bio-sensing analysis" @default.
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- W3136243045 doi "https://doi.org/10.1016/j.bios.2021.113125" @default.
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