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- W3136332112 abstract "AbstractModern DNA sequencing technologies have allowed for the sequencing of tens of thousands of bacterial genomes. While this explosion of information has brought about new insights into the diversity of the prokaryotic world, much less is known of the identity of proteins encoded within these genomes, as well as their rates of production. The advent of ribosome profiling, or the deep sequencing of ribosome-protected footprints, has recently enabled the systematic evaluation of every protein-coding region in a given experimental condition, the rates of protein production for each gene, and the variability in translation rates across each message. Here, I provide an update to the bacterial ribosome profiling approach, with a particular emphasis on a simplified strategy to reduce cloning time.Key wordsRibosome profilingRibo-seqBacterial ribosome profilingBacterial translation" @default.
- W3136332112 created "2021-03-29" @default.
- W3136332112 creator A5053468857 @default.
- W3136332112 date "2021-01-01" @default.
- W3136332112 modified "2023-09-27" @default.
- W3136332112 title "Monitoring Bacterial Translation Rates Genome-Wide" @default.
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- W3136332112 doi "https://doi.org/10.1007/978-1-0716-1150-0_1" @default.
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