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• W3136355077 endingPage "129614" @default.
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• W3136355077 abstract "• A simple QBs-based fluorescent strip immunosensor was developed for AFB 1 . • Improved fluorescent response was obtained with QBs as the highly luminescent amplification probes. • Visual and quantitative dual-readout for one run could be achieved within 15 min. • The immunosensor gave a LOD of 1 ng/mL for AFB 1 in a wide linear range of 1–19 ng/mL. • Excellent selectivity, stability and accuracy were confirmed by real application in lotus seeds. Owing to the serious threat of aflatoxin B 1 (AFB 1 ) to public health, development of a reliable method for accurate determination of it is extremely necessary and urgent. In this study, a simple, rapid and highly-sensitive quantum dot nanobeads (QBs) based lateral flow fluorescent strip immunosensor was developed for on-site detection of AFB 1 in edible and medicinal lotus seeds. Carboxylated QBs were used as the fluorescent markers to prepare the fluorescent probe through coupling QBs with anti-AFB1 antibodies. Bovine serum albumin (BSA)-AFB 1 antigens and goat anti-mouse IgG antibodies were coated on the nitrocellulose (NC) membrane to prepare the test (T) and control (C) lines, respectively. Qualitative analysis of AFB 1 was realized by naked eye, and the quantitative determination was achieved with a portable strip reader. Results showed that the newly-developed test strip sensor could achieve rapid detection of AFB 1 within 15 min, allowing a limit of detection (LOD) of 1 ng/mL (2 μg/kg) and a linear range of 1–19 ng/mL (2–38 μg/kg). Recovery rates from the fortified lotus seeds with low, medium and high spiking concentrations (2.5, 5 and 10 μg/kg) ranged from 94.0% to 116.0% with relative standard deviations less than 10%. All the results were confirmed by a standard LC-MS/MS method. The QBs-based fluorescent strip immunosensor with high sensitivity, easy operation, and low cost provided a preferred solution for rapid, on-site screening and highly-sensitive quantitation of AFB 1 in a large number of lotus seed samples." @default.
• W3136355077 created "2021-03-29" @default.
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• W3136355077 date "2021-09-01" @default.
• W3136355077 modified "2023-10-12" @default.
• W3136355077 title "Development of a quantum dot nanobead-based fluorescent strip immunosensor for on-site detection of aflatoxin B1 in lotus seeds" @default.
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• W3136355077 doi "https://doi.org/10.1016/j.foodchem.2021.129614" @default.
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