Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136358873> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W3136358873 abstract "Multidrug transporters can confer drug resistance on cells by extruding structurally unrelated compounds from the cellular interior. In transport assays, Hoechst 33342 (referred to as Hoechst) is a commonly used substrate, the fluorescence of which changes in the transport process. With three basic nitrogen atoms that can be protonated, Hoechst can exist as cationic and neutral species that have different fluorescence emissions and abilities to diffuse across cell envelopes and bind to lipids and intracellular nucleic acids. Due to this complexity, the mechanism of Hoechst transport by multidrug transporters is poorly characterised. We investigated Hoechst transport by the bacterial major facilitator superfamily multidrug-proton antiporter LmrP in Lactococcus lactis, and developed a novel assay for the direct quantitation of cell-associated Hoechst. We observe that changes in Hoechst fluorescence in cells do not always correlate with changes in the amount of Hoechst. Our data indicate that chemical proton gradient-dependent efflux by LmrP in cells converts populations of highly fluorescent, membrane-intercalated Hoechst in the alkaline interior into populations of less fluorescent, cell surface-bound Hoechst in the acidic exterior. Our methods and findings are directly relevant for the transport of many amphiphilic antibiotics, antineoplastic agents and cytotoxic compounds that are differentially protonated within the physiological pH range. The research data in this dataset record support the publication by Swain et al. in Scientific Reports and refer to the figures that are incorporated in the paper. Descriptions of the experimental details and statistical analyses are included in the Materials and Methods and figure legends of the paper." @default.
- W3136358873 created "2021-03-29" @default.
- W3136358873 creator A5019851198 @default.
- W3136358873 creator A5035486942 @default.
- W3136358873 creator A5038964630 @default.
- W3136358873 creator A5053772232 @default.
- W3136358873 creator A5053808561 @default.
- W3136358873 creator A5064533203 @default.
- W3136358873 date "2020-11-02" @default.
- W3136358873 modified "2023-09-26" @default.
- W3136358873 title "Research data supporting 'Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP'" @default.
- W3136358873 doi "https://doi.org/10.17863/cam.58079" @default.
- W3136358873 hasPublicationYear "2020" @default.
- W3136358873 type Work @default.
- W3136358873 sameAs 3136358873 @default.
- W3136358873 citedByCount "0" @default.
- W3136358873 crossrefType "dataset" @default.
- W3136358873 hasAuthorship W3136358873A5019851198 @default.
- W3136358873 hasAuthorship W3136358873A5035486942 @default.
- W3136358873 hasAuthorship W3136358873A5038964630 @default.
- W3136358873 hasAuthorship W3136358873A5053772232 @default.
- W3136358873 hasAuthorship W3136358873A5053808561 @default.
- W3136358873 hasAuthorship W3136358873A5064533203 @default.
- W3136358873 hasConcept C104317684 @default.
- W3136358873 hasConcept C12554922 @default.
- W3136358873 hasConcept C133936738 @default.
- W3136358873 hasConcept C145148216 @default.
- W3136358873 hasConcept C149011108 @default.
- W3136358873 hasConcept C178790620 @default.
- W3136358873 hasConcept C185592680 @default.
- W3136358873 hasConcept C18903297 @default.
- W3136358873 hasConcept C189613389 @default.
- W3136358873 hasConcept C200082930 @default.
- W3136358873 hasConcept C2777289219 @default.
- W3136358873 hasConcept C2910532024 @default.
- W3136358873 hasConcept C30095370 @default.
- W3136358873 hasConcept C41625074 @default.
- W3136358873 hasConcept C501593827 @default.
- W3136358873 hasConcept C55493867 @default.
- W3136358873 hasConcept C57181701 @default.
- W3136358873 hasConcept C57947595 @default.
- W3136358873 hasConcept C62465871 @default.
- W3136358873 hasConcept C86803240 @default.
- W3136358873 hasConcept C90424259 @default.
- W3136358873 hasConceptScore W3136358873C104317684 @default.
- W3136358873 hasConceptScore W3136358873C12554922 @default.
- W3136358873 hasConceptScore W3136358873C133936738 @default.
- W3136358873 hasConceptScore W3136358873C145148216 @default.
- W3136358873 hasConceptScore W3136358873C149011108 @default.
- W3136358873 hasConceptScore W3136358873C178790620 @default.
- W3136358873 hasConceptScore W3136358873C185592680 @default.
- W3136358873 hasConceptScore W3136358873C18903297 @default.
- W3136358873 hasConceptScore W3136358873C189613389 @default.
- W3136358873 hasConceptScore W3136358873C200082930 @default.
- W3136358873 hasConceptScore W3136358873C2777289219 @default.
- W3136358873 hasConceptScore W3136358873C2910532024 @default.
- W3136358873 hasConceptScore W3136358873C30095370 @default.
- W3136358873 hasConceptScore W3136358873C41625074 @default.
- W3136358873 hasConceptScore W3136358873C501593827 @default.
- W3136358873 hasConceptScore W3136358873C55493867 @default.
- W3136358873 hasConceptScore W3136358873C57181701 @default.
- W3136358873 hasConceptScore W3136358873C57947595 @default.
- W3136358873 hasConceptScore W3136358873C62465871 @default.
- W3136358873 hasConceptScore W3136358873C86803240 @default.
- W3136358873 hasConceptScore W3136358873C90424259 @default.
- W3136358873 hasLocation W31363588731 @default.
- W3136358873 hasOpenAccess W3136358873 @default.
- W3136358873 hasPrimaryLocation W31363588731 @default.
- W3136358873 hasRelatedWork W1027409293 @default.
- W3136358873 hasRelatedWork W1530020319 @default.
- W3136358873 hasRelatedWork W1798877632 @default.
- W3136358873 hasRelatedWork W1927502485 @default.
- W3136358873 hasRelatedWork W1980561868 @default.
- W3136358873 hasRelatedWork W2017491613 @default.
- W3136358873 hasRelatedWork W2028838362 @default.
- W3136358873 hasRelatedWork W2032388942 @default.
- W3136358873 hasRelatedWork W2038569576 @default.
- W3136358873 hasRelatedWork W2104791953 @default.
- W3136358873 hasRelatedWork W2113966671 @default.
- W3136358873 hasRelatedWork W2128460987 @default.
- W3136358873 hasRelatedWork W2145670383 @default.
- W3136358873 hasRelatedWork W2155347384 @default.
- W3136358873 hasRelatedWork W2210218255 @default.
- W3136358873 hasRelatedWork W2482269736 @default.
- W3136358873 hasRelatedWork W2518773635 @default.
- W3136358873 hasRelatedWork W2582763662 @default.
- W3136358873 hasRelatedWork W2738809026 @default.
- W3136358873 hasRelatedWork W2918317834 @default.
- W3136358873 isParatext "false" @default.
- W3136358873 isRetracted "false" @default.
- W3136358873 magId "3136358873" @default.
- W3136358873 workType "dataset" @default.