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- W3136382913 abstract "AbstractRibosome profiling is a powerful technique that enables researchers to monitor translational events across the transcriptome. It provides a snapshot of ribosome positions and density across the transcriptome at a sub-codon resolution. Here we describe the whole procedure of profiling ribosome footprints in mammalian cells. Two methods for Ribo-seq library construction are introduced, and their advantages and disadvantages are compared. There is a room for further improvement of Ribo-seq in terms of the amount of starting material, the duration of library construction, and the resolution of sequencing results.Key wordsRibo-seqTranslationmRNACodonDeep sequencing" @default.
- W3136382913 created "2021-03-29" @default.
- W3136382913 creator A5053334268 @default.
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- W3136382913 date "2021-01-01" @default.
- W3136382913 modified "2023-09-25" @default.
- W3136382913 title "Poly-A Tailing and Adaptor Ligation Methods for Ribo-Seq Library Construction" @default.
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- W3136382913 doi "https://doi.org/10.1007/978-1-0716-1150-0_10" @default.
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