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- W3136386588 startingPage "3123" @default.
- W3136386588 abstract "During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli. In molluscan shell, the myosin-chitin synthases have been suggested to be the link for this communication between cells and the biomaterial. Hyaluronan synthases (HAS) belong to the same enzyme family as chitin synthases. Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration. We hypothesize that HASes’ expression and activity are controlled by fluid-induced mechanotransduction as it is known for molluscan chitin synthases. In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa. The RNA transcriptome was analyzed by RNA sequencing (RNAseq). HA concentrations in the supernatants were measured by ELISA. The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy. Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways. The HAS activity appeared to be induced. Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment." @default.
- W3136386588 created "2021-03-29" @default.
- W3136386588 creator A5020973541 @default.
- W3136386588 creator A5032608774 @default.
- W3136386588 creator A5063306362 @default.
- W3136386588 creator A5074009771 @default.
- W3136386588 date "2021-03-18" @default.
- W3136386588 modified "2023-10-16" @default.
- W3136386588 title "Hyaluronan Synthases’ Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells" @default.
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- W3136386588 doi "https://doi.org/10.3390/ijms22063123" @default.
- W3136386588 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8003268" @default.
- W3136386588 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33803805" @default.
- W3136386588 hasPublicationYear "2021" @default.
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