FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136395279> ?p ?o ?g. }

• W3136395279 abstract "Abstract High-throughput sequencing of adaptive immune receptor repertoires is a valuable tool for receiving insights in adaptive immunity studies. Several powerful TCR/BCR repertoire reconstruction and analysis methods have been developed in the past decade. However, detecting and correcting the discrepancy between real and experimentally observed lymphocyte clone frequencies is still challenging. Here we discovered a hallmark anomaly in the ratio between read count and clone count-based frequencies of non-functional clonotypes in multiplex PCR-based immune repertoires. Calculating this anomaly, we formulated a quantitative measure of V- and J-genes frequency bias driven by multiplex PCR during library preparation called Over Amplification Rate (OAR). Based on the OAR concept, we developed an original software for multiplex PCR-specific bias evaluation and correction named iROAR: Immune Repertoire Over Amplification Removal ( https://github.com/smiranast/iROAR ). The iROAR algorithm was successfully tested on previously published TCR repertoires obtained using both 5’ RACE (Rapid Amplification of cDNA Ends)-based and multiplex PCR-based approaches and compared with a biological spike-in-based method for PCR bias evaluation. The developed approach can increase the accuracy and consistency of repertoires reconstructed by different methods making them more applicable for comparative analysis." @default.
• W3136395279 created "2021-03-29" @default.
• W3136395279 creator A5003011372 @default.
• W3136395279 creator A5018769333 @default.
• W3136395279 creator A5027407180 @default.
• W3136395279 creator A5060028352 @default.
• W3136395279 creator A5060040008 @default.
• W3136395279 creator A5062009698 @default.
• W3136395279 creator A5066151018 @default.
• W3136395279 creator A5066883165 @default.
• W3136395279 creator A5068428867 @default.
• W3136395279 date "2021-03-25" @default.
• W3136395279 modified "2023-10-16" @default.
• W3136395279 title "The use of non-functional clonotypes as a natural calibrator for quantitative bias correction in adaptive immune receptor repertoire profiling" @default.
• W3136395279 cites W2041390189 @default.
• W3136395279 cites W2043071551 @default.
• W3136395279 cites W2074640340 @default.
• W3136395279 cites W2089041106 @default.
• W3136395279 cites W2140323624 @default.
• W3136395279 cites W2167225983 @default.
• W3136395279 cites W2185265351 @default.
• W3136395279 cites W2263133791 @default.
• W3136395279 cites W2327217129 @default.
• W3136395279 cites W2405860911 @default.
• W3136395279 cites W2763557630 @default.
• W3136395279 cites W2763756177 @default.
• W3136395279 cites W2778215749 @default.
• W3136395279 cites W2779241561 @default.
• W3136395279 cites W2782901369 @default.
• W3136395279 cites W2789751302 @default.
• W3136395279 cites W2790709275 @default.
• W3136395279 cites W2792867842 @default.
• W3136395279 cites W2797380951 @default.
• W3136395279 cites W2925903995 @default.
• W3136395279 cites W2949095246 @default.
• W3136395279 cites W2951912016 @default.
• W3136395279 cites W2954189331 @default.
• W3136395279 cites W2955369607 @default.
• W3136395279 cites W2978327023 @default.
• W3136395279 cites W3008885059 @default.
• W3136395279 cites W3025534864 @default.
• W3136395279 cites W3049704595 @default.
• W3136395279 cites W3083146163 @default.
• W3136395279 cites W3184584468 @default.
• W3136395279 cites W4226391375 @default.
• W3136395279 cites W4283836483 @default.
• W3136395279 cites W4285491338 @default.
• W3136395279 cites W4293113787 @default.
• W3136395279 doi "https://doi.org/10.1101/2021.03.24.436794" @default.
• W3136395279 hasPublicationYear "2021" @default.
• W3136395279 type Work @default.
• W3136395279 sameAs 3136395279 @default.
• W3136395279 citedByCount "1" @default.
• W3136395279 countsByYear W31363952792022 @default.
• W3136395279 crossrefType "posted-content" @default.
• W3136395279 hasAuthorship W3136395279A5003011372 @default.
• W3136395279 hasAuthorship W3136395279A5018769333 @default.
• W3136395279 hasAuthorship W3136395279A5027407180 @default.
• W3136395279 hasAuthorship W3136395279A5060028352 @default.
• W3136395279 hasAuthorship W3136395279A5060040008 @default.
• W3136395279 hasAuthorship W3136395279A5062009698 @default.
• W3136395279 hasAuthorship W3136395279A5066151018 @default.
• W3136395279 hasAuthorship W3136395279A5066883165 @default.
• W3136395279 hasAuthorship W3136395279A5068428867 @default.
• W3136395279 hasBestOaLocation W31363952791 @default.
• W3136395279 hasConcept C121332964 @default.
• W3136395279 hasConcept C19317047 @default.
• W3136395279 hasConcept C193419808 @default.
• W3136395279 hasConcept C24890656 @default.
• W3136395279 hasConcept C2776090121 @default.
• W3136395279 hasConcept C2778473898 @default.
• W3136395279 hasConcept C2781188995 @default.
• W3136395279 hasConcept C54355233 @default.
• W3136395279 hasConcept C70721500 @default.
• W3136395279 hasConcept C86803240 @default.
• W3136395279 hasConcept C8891405 @default.
• W3136395279 hasConceptScore W3136395279C121332964 @default.
• W3136395279 hasConceptScore W3136395279C19317047 @default.
• W3136395279 hasConceptScore W3136395279C193419808 @default.
• W3136395279 hasConceptScore W3136395279C24890656 @default.
• W3136395279 hasConceptScore W3136395279C2776090121 @default.
• W3136395279 hasConceptScore W3136395279C2778473898 @default.
• W3136395279 hasConceptScore W3136395279C2781188995 @default.
• W3136395279 hasConceptScore W3136395279C54355233 @default.
• W3136395279 hasConceptScore W3136395279C70721500 @default.
• W3136395279 hasConceptScore W3136395279C86803240 @default.
• W3136395279 hasConceptScore W3136395279C8891405 @default.
• W3136395279 hasLocation W31363952791 @default.
• W3136395279 hasLocation W31363952792 @default.
• W3136395279 hasOpenAccess W3136395279 @default.
• W3136395279 hasPrimaryLocation W31363952791 @default.
• W3136395279 hasRelatedWork W2071150108 @default.
• W3136395279 hasRelatedWork W2728919089 @default.
• W3136395279 hasRelatedWork W2766374327 @default.
• W3136395279 hasRelatedWork W2952135630 @default.
• W3136395279 hasRelatedWork W2980787744 @default.
• W3136395279 hasRelatedWork W3136395279 @default.
• W3136395279 hasRelatedWork W4295007622 @default.
• W3136395279 hasRelatedWork W4309593122 @default.
• W3136395279 hasRelatedWork W4311013520 @default.

• next