FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136399136> ?p ?o ?g. }

• W3136399136 endingPage "e0242529" @default.
• W3136399136 startingPage "e0242529" @default.
• W3136399136 abstract "Precise quantification of molecular targets in a biological sample across a wide dynamic range is a key requirement in many diagnostic procedures, such as monitoring response to therapy or detection of measurable residual disease. State of the art digital PCR assays provide for a dynamic range of four orders of magnitude. However digital assays are complex and require sophisticated microfluidic tools. Here we present an assay format that enables ultra-precise quantification of RNA targets in a single measurement across a dynamic range of more than six orders of magnitude. The approach is based on hydrogel beads that provide for microfluidic free compartmentalization of the sample as they are used as nanoreactors for reverse transcription, PCR amplification and combined real time and digital detection of gene transcripts. We have applied these nanoreactor beads for establishing an assay for the detection and quantification of BCR-ABL1 fusion transcripts. The assay has been characterized for its precision and linear dynamic range. A comparison of the new method against conventional real time RT-PCR analysis (reference method) with clinical samples from patients with chronic myeloid leukemia (CML) revealed excellent concordance with Pearsons correlation coefficient of 0.983 and slope of 1.08." @default.
• W3136399136 created "2021-03-29" @default.
• W3136399136 creator A5011795958 @default.
• W3136399136 creator A5022040311 @default.
• W3136399136 creator A5031164592 @default.
• W3136399136 creator A5033375250 @default.
• W3136399136 creator A5035964802 @default.
• W3136399136 creator A5038048953 @default.
• W3136399136 creator A5049524439 @default.
• W3136399136 creator A5077360384 @default.
• W3136399136 creator A5082367688 @default.
• W3136399136 creator A5084153736 @default.
• W3136399136 date "2021-03-18" @default.
• W3136399136 modified "2023-09-30" @default.
• W3136399136 title "Ultra-precise quantification of mRNA targets across a broad dynamic range with nanoreactor beads" @default.
• W3136399136 cites W2036862498 @default.
• W3136399136 cites W2042672083 @default.
• W3136399136 cites W2074620807 @default.
• W3136399136 cites W2076924877 @default.
• W3136399136 cites W2077812841 @default.
• W3136399136 cites W2085380252 @default.
• W3136399136 cites W2093749485 @default.
• W3136399136 cites W2105666116 @default.
• W3136399136 cites W2109397680 @default.
• W3136399136 cites W2125376694 @default.
• W3136399136 cites W2141324982 @default.
• W3136399136 cites W2342971750 @default.
• W3136399136 cites W2476523730 @default.
• W3136399136 cites W2548958932 @default.
• W3136399136 cites W2613867010 @default.
• W3136399136 cites W2898995468 @default.
• W3136399136 cites W2903536326 @default.
• W3136399136 cites W2952930680 @default.
• W3136399136 cites W3009751715 @default.
• W3136399136 cites W4239139991 @default.
• W3136399136 doi "https://doi.org/10.1371/journal.pone.0242529" @default.
• W3136399136 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7971518" @default.
• W3136399136 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33735175" @default.
• W3136399136 hasPublicationYear "2021" @default.
• W3136399136 type Work @default.
• W3136399136 sameAs 3136399136 @default.
• W3136399136 citedByCount "1" @default.
• W3136399136 countsByYear W31363991362023 @default.
• W3136399136 crossrefType "journal-article" @default.
• W3136399136 hasAuthorship W3136399136A5011795958 @default.
• W3136399136 hasAuthorship W3136399136A5022040311 @default.
• W3136399136 hasAuthorship W3136399136A5031164592 @default.
• W3136399136 hasAuthorship W3136399136A5033375250 @default.
• W3136399136 hasAuthorship W3136399136A5035964802 @default.
• W3136399136 hasAuthorship W3136399136A5038048953 @default.
• W3136399136 hasAuthorship W3136399136A5049524439 @default.
• W3136399136 hasAuthorship W3136399136A5077360384 @default.
• W3136399136 hasAuthorship W3136399136A5082367688 @default.
• W3136399136 hasAuthorship W3136399136A5084153736 @default.
• W3136399136 hasBestOaLocation W31363991361 @default.
• W3136399136 hasConcept C104317684 @default.
• W3136399136 hasConcept C155672457 @default.
• W3136399136 hasConcept C171250308 @default.
• W3136399136 hasConcept C190818770 @default.
• W3136399136 hasConcept C192562407 @default.
• W3136399136 hasConcept C31972630 @default.
• W3136399136 hasConcept C41008148 @default.
• W3136399136 hasConcept C48126324 @default.
• W3136399136 hasConcept C49105822 @default.
• W3136399136 hasConcept C54355233 @default.
• W3136399136 hasConcept C70721500 @default.
• W3136399136 hasConcept C8673954 @default.
• W3136399136 hasConcept C86803240 @default.
• W3136399136 hasConcept C87133666 @default.
• W3136399136 hasConceptScore W3136399136C104317684 @default.
• W3136399136 hasConceptScore W3136399136C155672457 @default.
• W3136399136 hasConceptScore W3136399136C171250308 @default.
• W3136399136 hasConceptScore W3136399136C190818770 @default.
• W3136399136 hasConceptScore W3136399136C192562407 @default.
• W3136399136 hasConceptScore W3136399136C31972630 @default.
• W3136399136 hasConceptScore W3136399136C41008148 @default.
• W3136399136 hasConceptScore W3136399136C48126324 @default.
• W3136399136 hasConceptScore W3136399136C49105822 @default.
• W3136399136 hasConceptScore W3136399136C54355233 @default.
• W3136399136 hasConceptScore W3136399136C70721500 @default.
• W3136399136 hasConceptScore W3136399136C8673954 @default.
• W3136399136 hasConceptScore W3136399136C86803240 @default.
• W3136399136 hasConceptScore W3136399136C87133666 @default.
• W3136399136 hasFunder F4320338352 @default.
• W3136399136 hasIssue "3" @default.
• W3136399136 hasLocation W31363991361 @default.
• W3136399136 hasLocation W31363991362 @default.
• W3136399136 hasLocation W31363991363 @default.
• W3136399136 hasLocation W31363991364 @default.
• W3136399136 hasOpenAccess W3136399136 @default.
• W3136399136 hasPrimaryLocation W31363991361 @default.
• W3136399136 hasRelatedWork W2575688207 @default.
• W3136399136 hasRelatedWork W3044197901 @default.
• W3136399136 hasRelatedWork W3093604131 @default.
• W3136399136 hasRelatedWork W3097840689 @default.
• W3136399136 hasRelatedWork W3113310955 @default.
• W3136399136 hasRelatedWork W3118786857 @default.
• W3136399136 hasRelatedWork W3136399136 @default.

• next