FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136403448> ?p ?o ?g. }

• W3136403448 abstract "Abstract Protein is a major component of all biological evidence. Proteomic genotyping is the use of genetically variant peptides that contain single amino acid polymorphisms to infer the genotype of matching non-synonymous single nucleotide polymorphisms for the individual who originated the protein sample. This can be used to statistically associate an individual to evidence found at a crime scene. The utility of the inferred genotype increases as the detection of genetically variant peptides increases, which is the direct result of technology transfer to mass spectrometry platforms typically available. Digests of single (2 cm) human hair shafts from three European and two African subjects were analyzed using data dependent acquisition on a Q-Exactive™ Plus Hybrid Quadrupole-Orbitrap™ system, data independent acquisition and a variant of parallel reaction monitoring on a Orbitrap Fusion™ Lumos™ Tribrid™ system, and multiple reaction monitoring on an Agilent 6495 triple quadrupole system. In our hands, average genetically variant peptide detection from a selected 24 genetically variant peptide panel increased from 6.5 ± 1.1 and 3.1 ± 0.8 using data dependent and independent acquisition to 9.5 ± 0.7 and 11.7 ± 1.7 using parallel reaction monitoring and multiple reaction monitoring (p < 0.05). Parallel reaction monitoring resulted in a 1.3-fold increase in detection sensitivity, and multiple reaction monitoring resulted in a 1.6-fold increase in detection sensitivity. This increase in biomarker detection has a functional impact on the statistical association of a protein sample and an individual. Increased biomarker sensitivity, using Markov Chain Monte Carlo modeling, produced a median estimated random match probability of over 1 in 10 trillion from a single hair using targeted proteomics. For parallel reaction monitoring and multiple reaction monitoring, detected genetically variant peptides were validated by the inclusion of stable isotope labeled peptides in each sample, which served also as a detection trigger. This research accomplishes two aims: the demonstration of utility for alternative analytical platforms in proteomic genotyping, and the establishment of validation methods for the evaluation of inferred genotypes. Highlights Test four mass spectrometry configurations to optimize detection of genetically variant peptides Technology transfer of proteomic genotyping assays Improved sensitivity results in higher level of forensic discrimination for human identification using multiple reaction monitoring Graphical Abstract" @default.
• W3136403448 created "2021-03-29" @default.
• W3136403448 creator A5036703858 @default.
• W3136403448 creator A5037211990 @default.
• W3136403448 creator A5041109959 @default.
• W3136403448 creator A5049185721 @default.
• W3136403448 creator A5056712021 @default.
• W3136403448 creator A5071014691 @default.
• W3136403448 creator A5084209996 @default.
• W3136403448 date "2021-03-16" @default.
• W3136403448 modified "2023-09-27" @default.
• W3136403448 title "Alternative LC-MS/MS Platforms and Data Acquisition Strategies for Proteomic Genotyping of Human Hair Shafts" @default.
• W3136403448 cites W1568341953 @default.
• W3136403448 cites W1570931397 @default.
• W3136403448 cites W1824293040 @default.
• W3136403448 cites W1978639802 @default.
• W3136403448 cites W1995403127 @default.
• W3136403448 cites W2021171395 @default.
• W3136403448 cites W2031363300 @default.
• W3136403448 cites W2050547587 @default.
• W3136403448 cites W2056760934 @default.
• W3136403448 cites W2069364332 @default.
• W3136403448 cites W2072000480 @default.
• W3136403448 cites W2079843936 @default.
• W3136403448 cites W2079904970 @default.
• W3136403448 cites W2082006641 @default.
• W3136403448 cites W2098068920 @default.
• W3136403448 cites W2104549677 @default.
• W3136403448 cites W2107053633 @default.
• W3136403448 cites W2108047707 @default.
• W3136403448 cites W2111865342 @default.
• W3136403448 cites W2121171062 @default.
• W3136403448 cites W2126557729 @default.
• W3136403448 cites W2129390057 @default.
• W3136403448 cites W2136154336 @default.
• W3136403448 cites W2138309709 @default.
• W3136403448 cites W2141091417 @default.
• W3136403448 cites W2144718087 @default.
• W3136403448 cites W2151178734 @default.
• W3136403448 cites W2151619201 @default.
• W3136403448 cites W2162925720 @default.
• W3136403448 cites W2180913304 @default.
• W3136403448 cites W2276878166 @default.
• W3136403448 cites W2313734891 @default.
• W3136403448 cites W2417390730 @default.
• W3136403448 cites W2495555482 @default.
• W3136403448 cites W2520345180 @default.
• W3136403448 cites W2626970683 @default.
• W3136403448 cites W2803006006 @default.
• W3136403448 cites W2900872097 @default.
• W3136403448 cites W2912536238 @default.
• W3136403448 cites W2920981527 @default.
• W3136403448 cites W2930612746 @default.
• W3136403448 cites W2947183744 @default.
• W3136403448 cites W2947763854 @default.
• W3136403448 cites W2952907530 @default.
• W3136403448 cites W2967192801 @default.
• W3136403448 cites W2977000446 @default.
• W3136403448 cites W2981017496 @default.
• W3136403448 cites W2988286394 @default.
• W3136403448 cites W3008809691 @default.
• W3136403448 cites W3015356575 @default.
• W3136403448 cites W3016694036 @default.
• W3136403448 cites W3028898299 @default.
• W3136403448 cites W3029397410 @default.
• W3136403448 cites W4254185355 @default.
• W3136403448 cites W4300009529 @default.
• W3136403448 cites W87556421 @default.
• W3136403448 doi "https://doi.org/10.1101/2021.03.15.435505" @default.
• W3136403448 hasPublicationYear "2021" @default.
• W3136403448 type Work @default.
• W3136403448 sameAs 3136403448 @default.
• W3136403448 citedByCount "0" @default.
• W3136403448 crossrefType "posted-content" @default.
• W3136403448 hasAuthorship W3136403448A5036703858 @default.
• W3136403448 hasAuthorship W3136403448A5037211990 @default.
• W3136403448 hasAuthorship W3136403448A5041109959 @default.
• W3136403448 hasAuthorship W3136403448A5049185721 @default.
• W3136403448 hasAuthorship W3136403448A5056712021 @default.
• W3136403448 hasAuthorship W3136403448A5071014691 @default.
• W3136403448 hasAuthorship W3136403448A5084209996 @default.
• W3136403448 hasBestOaLocation W31364034481 @default.
• W3136403448 hasConcept C104317684 @default.
• W3136403448 hasConcept C135763542 @default.
• W3136403448 hasConcept C154945302 @default.
• W3136403448 hasConcept C162356407 @default.
• W3136403448 hasConcept C185592680 @default.
• W3136403448 hasConcept C2780841973 @default.
• W3136403448 hasConcept C2781197716 @default.
• W3136403448 hasConcept C31467283 @default.
• W3136403448 hasConcept C31827203 @default.
• W3136403448 hasConcept C41008148 @default.
• W3136403448 hasConcept C43617362 @default.
• W3136403448 hasConcept C55493867 @default.
• W3136403448 hasConcept C64869954 @default.
• W3136403448 hasConcept C70721500 @default.
• W3136403448 hasConcept C71043704 @default.
• W3136403448 hasConcept C86803240 @default.
• W3136403448 hasConceptScore W3136403448C104317684 @default.
• W3136403448 hasConceptScore W3136403448C135763542 @default.

• next