FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136759775> ?p ?o ?g. }

• W3136759775 endingPage "686" @default.
• W3136759775 startingPage "677" @default.
• W3136759775 abstract "For Pseudomonas aeruginosa (PA), infection control and appropriate antimicrobial treatment have become important issues. Diagnosis is critical in managing PA infection, but conventional methods are not highly accurate or rapid. We developed a new PA quantification system based on 23S rRNA-targeted reverse transcription quantitative PCR (RT-qPCR). We confirmed that RT-qPCR can quantify PA directly from clinical samples quickly (within 6 h) and with high sensitivity (blood, 1 cell/mL; stool, 100 cells/g) and without cross-reaction. Also, under antibiotic treatment, PA viable counts detected by this system correlated well with the inflammatory response of infected Caco-2 cells compared to other methods such as culturing and qPCR. Next, we utilized this system on fecal samples collected from 65 septic ICU patients and 44 healthy volunteers to identify ICU infection status. We confirmed that the PA detection ratio in ICU patients was significantly higher than that in healthy volunteers (49.2% vs. 13.6%, P < 0.05). Additionally, we monitored drug-resistant PA in 4 ICU patients by this system. The trends in PA counts accurately reflected various treatment backgrounds such as antibiotic use and mechanical ventilator use. Our results suggest that this RT-qPCR system is beneficial for the early diagnosis and evaluation of appropriate antibacterial treatment and may be a useful tool in combating PA infection." @default.
• W3136759775 created "2021-03-29" @default.
• W3136759775 creator A5009335642 @default.
• W3136759775 creator A5026642736 @default.
• W3136759775 creator A5035543462 @default.
• W3136759775 creator A5044245317 @default.
• W3136759775 creator A5050533134 @default.
• W3136759775 creator A5061247668 @default.
• W3136759775 creator A5075291713 @default.
• W3136759775 creator A5077641003 @default.
• W3136759775 creator A5085794430 @default.
• W3136759775 date "2021-01-01" @default.
• W3136759775 modified "2023-10-18" @default.
• W3136759775 title "Development of a rapid and sensitive analytical system for <i>Pseudomonas aeruginosa</i> based on reverse transcription quantitative PCR targeting of rRNA molecules" @default.
• W3136759775 cites W1489792556 @default.
• W3136759775 cites W1863631349 @default.
• W3136759775 cites W1972020615 @default.
• W3136759775 cites W1977455677 @default.
• W3136759775 cites W1987447861 @default.
• W3136759775 cites W1987525683 @default.
• W3136759775 cites W1994687102 @default.
• W3136759775 cites W2006375923 @default.
• W3136759775 cites W2014459325 @default.
• W3136759775 cites W2024263059 @default.
• W3136759775 cites W2043721355 @default.
• W3136759775 cites W2058653685 @default.
• W3136759775 cites W2071605567 @default.
• W3136759775 cites W2086605687 @default.
• W3136759775 cites W2097382368 @default.
• W3136759775 cites W2100441604 @default.
• W3136759775 cites W2106948630 @default.
• W3136759775 cites W2143366420 @default.
• W3136759775 cites W2155136011 @default.
• W3136759775 cites W2170156996 @default.
• W3136759775 cites W2285906411 @default.
• W3136759775 cites W2303008909 @default.
• W3136759775 cites W2735842795 @default.
• W3136759775 cites W2778781692 @default.
• W3136759775 cites W2803953825 @default.
• W3136759775 cites W2805372352 @default.
• W3136759775 cites W2807161463 @default.
• W3136759775 cites W2894132774 @default.
• W3136759775 cites W2899513950 @default.
• W3136759775 cites W2900742823 @default.
• W3136759775 cites W2919801676 @default.
• W3136759775 cites W2970103499 @default.
• W3136759775 cites W3082938003 @default.
• W3136759775 doi "https://doi.org/10.1080/22221751.2021.1906164" @default.
• W3136759775 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8023615" @default.
• W3136759775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33734032" @default.
• W3136759775 hasPublicationYear "2021" @default.
• W3136759775 type Work @default.
• W3136759775 sameAs 3136759775 @default.
• W3136759775 citedByCount "6" @default.
• W3136759775 countsByYear W31367597752021 @default.
• W3136759775 countsByYear W31367597752022 @default.
• W3136759775 countsByYear W31367597752023 @default.
• W3136759775 crossrefType "journal-article" @default.
• W3136759775 hasAuthorship W3136759775A5009335642 @default.
• W3136759775 hasAuthorship W3136759775A5026642736 @default.
• W3136759775 hasAuthorship W3136759775A5035543462 @default.
• W3136759775 hasAuthorship W3136759775A5044245317 @default.
• W3136759775 hasAuthorship W3136759775A5050533134 @default.
• W3136759775 hasAuthorship W3136759775A5061247668 @default.
• W3136759775 hasAuthorship W3136759775A5075291713 @default.
• W3136759775 hasAuthorship W3136759775A5077641003 @default.
• W3136759775 hasAuthorship W3136759775A5085794430 @default.
• W3136759775 hasBestOaLocation W31367597751 @default.
• W3136759775 hasConcept C104317684 @default.
• W3136759775 hasConcept C105580179 @default.
• W3136759775 hasConcept C156719811 @default.
• W3136759775 hasConcept C2777637488 @default.
• W3136759775 hasConcept C40749294 @default.
• W3136759775 hasConcept C4692481 @default.
• W3136759775 hasConcept C48023723 @default.
• W3136759775 hasConcept C49105822 @default.
• W3136759775 hasConcept C4937899 @default.
• W3136759775 hasConcept C501593827 @default.
• W3136759775 hasConcept C523546767 @default.
• W3136759775 hasConcept C54355233 @default.
• W3136759775 hasConcept C55493867 @default.
• W3136759775 hasConcept C67705224 @default.
• W3136759775 hasConcept C71924100 @default.
• W3136759775 hasConcept C86803240 @default.
• W3136759775 hasConcept C88478588 @default.
• W3136759775 hasConcept C89423630 @default.
• W3136759775 hasConceptScore W3136759775C104317684 @default.
• W3136759775 hasConceptScore W3136759775C105580179 @default.
• W3136759775 hasConceptScore W3136759775C156719811 @default.
• W3136759775 hasConceptScore W3136759775C2777637488 @default.
• W3136759775 hasConceptScore W3136759775C40749294 @default.
• W3136759775 hasConceptScore W3136759775C4692481 @default.
• W3136759775 hasConceptScore W3136759775C48023723 @default.
• W3136759775 hasConceptScore W3136759775C49105822 @default.
• W3136759775 hasConceptScore W3136759775C4937899 @default.
• W3136759775 hasConceptScore W3136759775C501593827 @default.
• W3136759775 hasConceptScore W3136759775C523546767 @default.
• W3136759775 hasConceptScore W3136759775C54355233 @default.

• next