FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136770185> ?p ?o ?g. }

• W3136770185 endingPage "9" @default.
• W3136770185 startingPage "1" @default.
• W3136770185 abstract "We aimed to evaluate the effect of activated platelet-rich plasma (PRP) on proliferation and chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Six mature male rabbits were included in this study. PRP was obtained by two-step centrifugation from whole blood, and it was activated using CaCl2 solution. BMSCs were isolated and proliferated from bone marrow of rabbits and characterized by flow cytometry. Passage 3 BMSCs were cultured in high-glucose Dulbecco’s modified Eagle’s medium (HG-DMEM) with the four different compositions for consecutive 7 days, including 10% fetal bovine serum, 5% PRP, 10% PRP, and 15% PRP. Cell counting assays were performed to evaluate the cell proliferation of BMSCs. BMSCs ( <math xmlns=http://www.w3.org/1998/Math/MathML id=M1> <mn>5</mn> <mo>×</mo> <msup> <mrow> <mn>10</mn> </mrow> <mrow> <mn>5</mn> </mrow> </msup> </math> cells/well in 6-well plates) were induced in four conditions for 21 days to chondrogenic differentiation evaluation, including commercial chondrogenic medium (control), 5% PRP (HG-DMEM+5% PRP), 10% PRP (HG-DMEM+10% PRP), and 15% PRP (HG-DMEM+15% PRP). The gene expression levels of ACAN, COL2A1, and SOX9 in pellets were detected. Morphological and pathological assessments were performed by the blind observer. After purifying, the percentages of cells with CD105(+)/CD34(−) and CD44(+)/CD45(−) were 96.5% and 92.9%, respectively. The proliferation of BMSCs was enhanced in all groups, and 10% PRP revealed more significant outcome than the others from day 5. The levels of ACAN, COL2A1, and SOX9 were lower in the three PRP groups than control group, but the levels of ACAN and SOX9 were higher in 10% PRP group than 5% and 15% PRP groups. Histological examinations showed that 10% PRP-treated pellets had more regular appearance, larger size, and abundant extracellular matrix than 5% or 10% PRP groups, but still inferior to commercial chondrogenic medium. In conclusion, our results show that PRP may enhance the proliferation of rabbit BMSCs. However, PRP have limited effect on chondrogenic differentiation in comparison with commercial chondrogenic medium in pellets culture." @default.
• W3136770185 created "2021-03-29" @default.
• W3136770185 creator A5020985581 @default.
• W3136770185 creator A5040171366 @default.
• W3136770185 creator A5046881277 @default.
• W3136770185 creator A5057019311 @default.
• W3136770185 creator A5066364830 @default.
• W3136770185 creator A5070931010 @default.
• W3136770185 date "2021-08-25" @default.
• W3136770185 modified "2023-10-15" @default.
• W3136770185 title "Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells" @default.
• W3136770185 cites W1504358537 @default.
• W3136770185 cites W1564650535 @default.
• W3136770185 cites W1935950008 @default.
• W3136770185 cites W1985028070 @default.
• W3136770185 cites W2006050540 @default.
• W3136770185 cites W2012504160 @default.
• W3136770185 cites W2020466745 @default.
• W3136770185 cites W2024745401 @default.
• W3136770185 cites W2037328562 @default.
• W3136770185 cites W2066155149 @default.
• W3136770185 cites W2127014553 @default.
• W3136770185 cites W2129728788 @default.
• W3136770185 cites W2156230245 @default.
• W3136770185 cites W2160076833 @default.
• W3136770185 cites W2166980716 @default.
• W3136770185 cites W2288918943 @default.
• W3136770185 cites W2331913619 @default.
• W3136770185 cites W2519793735 @default.
• W3136770185 cites W2768591340 @default.
• W3136770185 cites W2770532344 @default.
• W3136770185 cites W2773155538 @default.
• W3136770185 cites W2789811840 @default.
• W3136770185 cites W2792620317 @default.
• W3136770185 cites W2792942348 @default.
• W3136770185 cites W2805481751 @default.
• W3136770185 cites W2884782383 @default.
• W3136770185 cites W2891082241 @default.
• W3136770185 cites W2954085356 @default.
• W3136770185 cites W3015802834 @default.
• W3136770185 cites W3021737498 @default.
• W3136770185 cites W89020305 @default.
• W3136770185 doi "https://doi.org/10.1155/2021/9947187" @default.
• W3136770185 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8413060" @default.
• W3136770185 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34484349" @default.
• W3136770185 hasPublicationYear "2021" @default.
• W3136770185 type Work @default.
• W3136770185 sameAs 3136770185 @default.
• W3136770185 citedByCount "4" @default.
• W3136770185 countsByYear W31367701852022 @default.
• W3136770185 countsByYear W31367701852023 @default.
• W3136770185 crossrefType "journal-article" @default.
• W3136770185 hasAuthorship W3136770185A5020985581 @default.
• W3136770185 hasAuthorship W3136770185A5040171366 @default.
• W3136770185 hasAuthorship W3136770185A5046881277 @default.
• W3136770185 hasAuthorship W3136770185A5057019311 @default.
• W3136770185 hasAuthorship W3136770185A5066364830 @default.
• W3136770185 hasAuthorship W3136770185A5070931010 @default.
• W3136770185 hasBestOaLocation W31367701851 @default.
• W3136770185 hasConcept C10205521 @default.
• W3136770185 hasConcept C1491633281 @default.
• W3136770185 hasConcept C153911025 @default.
• W3136770185 hasConcept C16685009 @default.
• W3136770185 hasConcept C185592680 @default.
• W3136770185 hasConcept C198826908 @default.
• W3136770185 hasConcept C203014093 @default.
• W3136770185 hasConcept C2780007613 @default.
• W3136770185 hasConcept C2780076745 @default.
• W3136770185 hasConcept C2780932548 @default.
• W3136770185 hasConcept C28328180 @default.
• W3136770185 hasConcept C35496256 @default.
• W3136770185 hasConcept C553184892 @default.
• W3136770185 hasConcept C55493867 @default.
• W3136770185 hasConcept C71924100 @default.
• W3136770185 hasConcept C86803240 @default.
• W3136770185 hasConcept C89560881 @default.
• W3136770185 hasConcept C95444343 @default.
• W3136770185 hasConceptScore W3136770185C10205521 @default.
• W3136770185 hasConceptScore W3136770185C1491633281 @default.
• W3136770185 hasConceptScore W3136770185C153911025 @default.
• W3136770185 hasConceptScore W3136770185C16685009 @default.
• W3136770185 hasConceptScore W3136770185C185592680 @default.
• W3136770185 hasConceptScore W3136770185C198826908 @default.
• W3136770185 hasConceptScore W3136770185C203014093 @default.
• W3136770185 hasConceptScore W3136770185C2780007613 @default.
• W3136770185 hasConceptScore W3136770185C2780076745 @default.
• W3136770185 hasConceptScore W3136770185C2780932548 @default.
• W3136770185 hasConceptScore W3136770185C28328180 @default.
• W3136770185 hasConceptScore W3136770185C35496256 @default.
• W3136770185 hasConceptScore W3136770185C553184892 @default.
• W3136770185 hasConceptScore W3136770185C55493867 @default.
• W3136770185 hasConceptScore W3136770185C71924100 @default.
• W3136770185 hasConceptScore W3136770185C86803240 @default.
• W3136770185 hasConceptScore W3136770185C89560881 @default.
• W3136770185 hasConceptScore W3136770185C95444343 @default.
• W3136770185 hasFunder F4320321001 @default.
• W3136770185 hasLocation W31367701851 @default.
• W3136770185 hasLocation W31367701852 @default.

• next