FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136899227> ?p ?o ?g. }

• W3136899227 abstract "Small secreted proteins (SSPs), particularly cysteine-rich proteins secreted during fungal infection, comprise virulence effectors in plant-pathogenic fungi but remain unknown in insect-pathogenic fungi. We report here that only a small cysteine-free protein (CFP) is indispensable for insect pathogenicity of Beauveria bassiana among 10 studied SSPs (99 to 274 amino acids [aa]), including seven hypothetical proteins containing 0 to 12 Cys residues. CFP (120 aa) features an N-terminal signal peptide (residues 1 to 17), a nuclear localization signal motif (residues 24 to 57), and no predictable domain. Its homologs exist exclusively in insect-pathogenic Cordycipitaceae and Clavicipitaceae. Fluorescence-tagged CFP fusion protein was localized in the nucleus but extracellularly undetectable, suggesting an inability for CFP to be secreted out. Disruption of cfp resulted in abolished pathogenicity via normal cuticle infection, attenuated virulence via hemocoel injection, compromised conidiation capacity versus little growth defect, impaired conidial coat, blocked secretion of cuticle-degrading enzymes, impeded proliferation in vivo, disturbed cell cycle, reduced stress tolerance, and 1,818 dysregulated genes (genomic 17.54%). Hundreds of those genes correlated with phenotypic changes observed in the disruption mutant. Intriguingly, nearly 40% of those dysregulated genes encode hypothetical or unknown proteins, and another 13% encode transcription factors and enzymes or proteins collectively involved in genome-wide gene regulation. However, purified CFP showed no DNA-binding activity in an electrophoretic mobility shift assay. These findings unveil that CFP is a novel regulator of fungal insect-pathogenic life cycle and genomic expression and that cysteine richness is dispensable for distinguishing virulence effectors from putative SSPs in B. bassianaIMPORTANCE Small cysteine-rich proteins secreted during plant-pathogenic fungal infection comprise virulence effectors. Our study confirms that only a cysteine-free protein (CFP) is determinant to insect-pathogenic fungal virulence among 10 small putatively secreted proteins containing 0 to 12 Cys residues. Disruption of cfp abolished insect pathogenicity and caused not only a series of compromised cellular events associated with host infection and disease development but also dysregulation of 1,818 genes, although no DNA-binding activity was detected in purified CFP samples. Nearly 13% of those genes encode transcription factors and enzymes or proteins collectively involved in transcriptional regulation. Altogether, CFP serves as a novel regulator of the fungal insect-pathogenic life cycle and genomic expression. Cysteine richness is dispensable for distinguishing virulence effectors from the fungal SSPs." @default.
• W3136899227 created "2021-03-29" @default.
• W3136899227 creator A5000414718 @default.
• W3136899227 creator A5019670033 @default.
• W3136899227 creator A5036254678 @default.
• W3136899227 creator A5038987803 @default.
• W3136899227 creator A5082070951 @default.
• W3136899227 date "2021-04-27" @default.
• W3136899227 modified "2023-10-06" @default.
• W3136899227 title "A Small Cysteine-Free Protein Acts as a Novel Regulator of Fungal Insect-Pathogenic Lifecycle and Genomic Expression" @default.
• W3136899227 cites W1500890992 @default.
• W3136899227 cites W1572760104 @default.
• W3136899227 cites W1867307269 @default.
• W3136899227 cites W1965985446 @default.
• W3136899227 cites W1976260744 @default.
• W3136899227 cites W1981429455 @default.
• W3136899227 cites W1984647664 @default.
• W3136899227 cites W2002462368 @default.
• W3136899227 cites W2009035529 @default.
• W3136899227 cites W2015391559 @default.
• W3136899227 cites W2029835563 @default.
• W3136899227 cites W2032602147 @default.
• W3136899227 cites W2033241764 @default.
• W3136899227 cites W2046079276 @default.
• W3136899227 cites W2061803253 @default.
• W3136899227 cites W2093458353 @default.
• W3136899227 cites W2105909024 @default.
• W3136899227 cites W2108512366 @default.
• W3136899227 cites W2123754903 @default.
• W3136899227 cites W2126524332 @default.
• W3136899227 cites W2141687369 @default.
• W3136899227 cites W2153301596 @default.
• W3136899227 cites W2169075954 @default.
• W3136899227 cites W2170546657 @default.
• W3136899227 cites W2217359279 @default.
• W3136899227 cites W2237212576 @default.
• W3136899227 cites W2243012910 @default.
• W3136899227 cites W2262970286 @default.
• W3136899227 cites W2322420990 @default.
• W3136899227 cites W2341636404 @default.
• W3136899227 cites W2343407383 @default.
• W3136899227 cites W2517323174 @default.
• W3136899227 cites W2559191318 @default.
• W3136899227 cites W2578836999 @default.
• W3136899227 cites W2616907229 @default.
• W3136899227 cites W2618688312 @default.
• W3136899227 cites W2750535543 @default.
• W3136899227 cites W2763334496 @default.
• W3136899227 cites W2766664828 @default.
• W3136899227 cites W2789502670 @default.
• W3136899227 cites W2791550055 @default.
• W3136899227 cites W2801960533 @default.
• W3136899227 cites W2807738830 @default.
• W3136899227 cites W2893719315 @default.
• W3136899227 cites W2901948631 @default.
• W3136899227 cites W2930747851 @default.
• W3136899227 cites W2960786106 @default.
• W3136899227 cites W2969400055 @default.
• W3136899227 cites W3006099483 @default.
• W3136899227 cites W3008363758 @default.
• W3136899227 cites W3014857781 @default.
• W3136899227 cites W3017180120 @default.
• W3136899227 cites W3044736460 @default.
• W3136899227 cites W3045241889 @default.
• W3136899227 cites W3092645746 @default.
• W3136899227 doi "https://doi.org/10.1128/msystems.00098-21" @default.
• W3136899227 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8546967" @default.
• W3136899227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33758028" @default.
• W3136899227 hasPublicationYear "2021" @default.
• W3136899227 type Work @default.
• W3136899227 sameAs 3136899227 @default.
• W3136899227 citedByCount "12" @default.
• W3136899227 countsByYear W31368992272021 @default.
• W3136899227 countsByYear W31368992272022 @default.
• W3136899227 countsByYear W31368992272023 @default.
• W3136899227 crossrefType "journal-article" @default.
• W3136899227 hasAuthorship W3136899227A5000414718 @default.
• W3136899227 hasAuthorship W3136899227A5019670033 @default.
• W3136899227 hasAuthorship W3136899227A5036254678 @default.
• W3136899227 hasAuthorship W3136899227A5038987803 @default.
• W3136899227 hasAuthorship W3136899227A5082070951 @default.
• W3136899227 hasBestOaLocation W31368992271 @default.
• W3136899227 hasConcept C104317684 @default.
• W3136899227 hasConcept C10858879 @default.
• W3136899227 hasConcept C143065580 @default.
• W3136899227 hasConcept C167625842 @default.
• W3136899227 hasConcept C2779347548 @default.
• W3136899227 hasConcept C54355233 @default.
• W3136899227 hasConcept C55493867 @default.
• W3136899227 hasConcept C60987743 @default.
• W3136899227 hasConcept C86803240 @default.
• W3136899227 hasConcept C95444343 @default.
• W3136899227 hasConceptScore W3136899227C104317684 @default.
• W3136899227 hasConceptScore W3136899227C10858879 @default.
• W3136899227 hasConceptScore W3136899227C143065580 @default.
• W3136899227 hasConceptScore W3136899227C167625842 @default.
• W3136899227 hasConceptScore W3136899227C2779347548 @default.
• W3136899227 hasConceptScore W3136899227C54355233 @default.
• W3136899227 hasConceptScore W3136899227C55493867 @default.
• W3136899227 hasConceptScore W3136899227C60987743 @default.

• next