Matches in SemOpenAlex for { <https://semopenalex.org/work/W3136994990> ?p ?o ?g. }
- W3136994990 endingPage "2062" @default.
- W3136994990 startingPage "2050" @default.
- W3136994990 abstract "Abstract DNA barcoding and metabarcoding provide new avenues for investigating biological systems. These techniques require well‐curated reference libraries with extensive coverage. Generating an exhaustive national DNA barcode reference library can open up new avenues of research in ecology, evolution and conservation, yet few studies to date have created such a resource. In plant DNA barcoding, herbarium collections provide taxonomically robust material but also pose challenges in lab processing. Here, we present a national DNA barcoding resource covering all of the native flowering plants and conifers of the United Kingdom. This represents 1,482 plant species, with the majority of specimens (81%) sourced from herbaria. Using Sanger sequencing of the plant DNA barcode markers, rbcL , matK , and ITS2, at least one DNA barcode was retrieved from 98% of the UK flora. We sampled from multiple individuals, resulting in a species coverage for rbcL of 96% (4,477 sequences), 90% for matK (3,259 sequences) and 75% for ITS2 (2,585 sequences). Sequence recovery was lower for herbarium material compared to fresh collections, with the age of the specimen having a significant effect on the success of sequence recovery. Species level discrimination was highest with ITS2, however, the ability to successfully retrieve a sequence was lowest for this region. Analyses of the genetic distinctiveness of species across a complete flora showed DNA barcoding to be informative for all but the most taxonomically complex groups. The UK flora DNA barcode reference library provides an important resource for many applications that require plant identification from DNA." @default.
- W3136994990 created "2021-03-29" @default.
- W3136994990 creator A5004582589 @default.
- W3136994990 creator A5019431605 @default.
- W3136994990 creator A5022720735 @default.
- W3136994990 creator A5031683453 @default.
- W3136994990 creator A5040964298 @default.
- W3136994990 creator A5053159445 @default.
- W3136994990 creator A5055527092 @default.
- W3136994990 creator A5067440842 @default.
- W3136994990 creator A5084774073 @default.
- W3136994990 creator A5087522033 @default.
- W3136994990 creator A5090928688 @default.
- W3136994990 date "2021-04-03" @default.
- W3136994990 modified "2023-10-18" @default.
- W3136994990 title "Barcode UK: A complete DNA barcoding resource for the flowering plants and conifers of the United Kingdom" @default.
- W3136994990 cites W1926731616 @default.
- W3136994990 cites W1933869753 @default.
- W3136994990 cites W1977167384 @default.
- W3136994990 cites W1995507123 @default.
- W3136994990 cites W1999469035 @default.
- W3136994990 cites W2023356179 @default.
- W3136994990 cites W2042972043 @default.
- W3136994990 cites W2059949285 @default.
- W3136994990 cites W2064445073 @default.
- W3136994990 cites W2071510336 @default.
- W3136994990 cites W2074822895 @default.
- W3136994990 cites W2093296928 @default.
- W3136994990 cites W2099901798 @default.
- W3136994990 cites W2111536597 @default.
- W3136994990 cites W2127479578 @default.
- W3136994990 cites W2128523480 @default.
- W3136994990 cites W2144785518 @default.
- W3136994990 cites W2156935855 @default.
- W3136994990 cites W2167406761 @default.
- W3136994990 cites W2180283144 @default.
- W3136994990 cites W2337603118 @default.
- W3136994990 cites W2474124041 @default.
- W3136994990 cites W2495651450 @default.
- W3136994990 cites W2499213204 @default.
- W3136994990 cites W2526642232 @default.
- W3136994990 cites W2568667547 @default.
- W3136994990 cites W2588270181 @default.
- W3136994990 cites W2735056152 @default.
- W3136994990 cites W2754769271 @default.
- W3136994990 cites W2755095225 @default.
- W3136994990 cites W2762297863 @default.
- W3136994990 cites W2778546216 @default.
- W3136994990 cites W2785846143 @default.
- W3136994990 cites W2790484492 @default.
- W3136994990 cites W2791187584 @default.
- W3136994990 cites W2799983313 @default.
- W3136994990 cites W2807347642 @default.
- W3136994990 cites W2891201368 @default.
- W3136994990 cites W2911724990 @default.
- W3136994990 cites W2913641542 @default.
- W3136994990 cites W2919018280 @default.
- W3136994990 cites W2926647172 @default.
- W3136994990 cites W2935324402 @default.
- W3136994990 cites W2950884802 @default.
- W3136994990 cites W2951968263 @default.
- W3136994990 cites W2959429462 @default.
- W3136994990 cites W2980008431 @default.
- W3136994990 cites W3014928134 @default.
- W3136994990 cites W3017342212 @default.
- W3136994990 cites W3090484420 @default.
- W3136994990 cites W3121566389 @default.
- W3136994990 doi "https://doi.org/10.1111/1755-0998.13388" @default.
- W3136994990 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33749162" @default.
- W3136994990 hasPublicationYear "2021" @default.
- W3136994990 type Work @default.
- W3136994990 sameAs 3136994990 @default.
- W3136994990 citedByCount "23" @default.
- W3136994990 countsByYear W31369949902021 @default.
- W3136994990 countsByYear W31369949902022 @default.
- W3136994990 countsByYear W31369949902023 @default.
- W3136994990 crossrefType "journal-article" @default.
- W3136994990 hasAuthorship W3136994990A5004582589 @default.
- W3136994990 hasAuthorship W3136994990A5019431605 @default.
- W3136994990 hasAuthorship W3136994990A5022720735 @default.
- W3136994990 hasAuthorship W3136994990A5031683453 @default.
- W3136994990 hasAuthorship W3136994990A5040964298 @default.
- W3136994990 hasAuthorship W3136994990A5053159445 @default.
- W3136994990 hasAuthorship W3136994990A5055527092 @default.
- W3136994990 hasAuthorship W3136994990A5067440842 @default.
- W3136994990 hasAuthorship W3136994990A5084774073 @default.
- W3136994990 hasAuthorship W3136994990A5087522033 @default.
- W3136994990 hasAuthorship W3136994990A5090928688 @default.
- W3136994990 hasBestOaLocation W31369949901 @default.
- W3136994990 hasConcept C111919701 @default.
- W3136994990 hasConcept C141732470 @default.
- W3136994990 hasConcept C18903297 @default.
- W3136994990 hasConcept C206345919 @default.
- W3136994990 hasConcept C2776841711 @default.
- W3136994990 hasConcept C31258907 @default.
- W3136994990 hasConcept C41008148 @default.
- W3136994990 hasConcept C51679486 @default.
- W3136994990 hasConcept C54355233 @default.