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- W3137160626 abstract "Glyoxylate is an important chemical and is also an intermediate involved in metabolic pathways of living microorganisms. However, it cannot be rapidly utilized by many microbes. We observed a very long lag phase (up to 120 h) when E. coli is growing in a mineral medium supplemented with 50 mM glyoxylate. To better understand this strange growth pattern on glyoxylate and accelerate glyoxylate utilization, a random genomic library of E. coli was transformed into E. coli BW25113, and mutants that showed significantly shortened lag phase on glyoxylate were obtained. Interestingly, mutations in BtsT/BtsS, a two component system that is involved in pyruvate transport, were found to be a common feature in all mutants retrieved. We further demonstrated, through reverse engineering, that the mutations in BtsT/BtsS can indeed increase glyoxylate uptake. Growth experiments with different concentration of glyoxylate also showed the higher the concentration of glyoxylate, the shorter the lag phase. These new findings thus increased our understanding on microbial utilization of glyoxylate. • Using genomic library, E. coli mutants that showed significantly shortened lag phase on glyoxylate were obtained. • BtsT/BtsS is involved in glyoxylate transport in E. coli and its mutations facilitated glyoxylate utilization. • Growth experiments showed that the higher the concentration of glycolate, the shorter the lag phase." @default.
- W3137160626 created "2021-03-29" @default.
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- W3137160626 date "2021-04-01" @default.
- W3137160626 modified "2023-09-23" @default.
- W3137160626 title "BtsT/ BtsS is involved in glyoxylate transport in E. coli and its mutations facilitated glyoxylate utilization" @default.
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- W3137160626 doi "https://doi.org/10.1016/j.bbrc.2021.03.002" @default.
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