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• W3137184831 endingPage "3578" @default.
• W3137184831 startingPage "3571" @default.
• W3137184831 abstract "Herein, a highly selective and sensitive OFF-ON fluorescent biosensor was designed for intracellular Cu2+ detection. Compared to the fluorescent Cu2+ biosensors reported so far, this work tackled the tricky issue of reliability of Cu2+, which mainly depends on the integration of the high selectivity of the Cu(I)-catalyzed click reaction with the ultrahigh sensitivity of a spherical nucleic acid-based 3D DNA walker. Typically, DNA track is carried out by coconjugating N3-S1 and Cy3-HP onto gold nanoparticles (AuNPs). On this state, fluorophore (Cy3) was close to the surface of AuNPs (as a nanoquencher), generating a quenched fluorescence and thus causing the initial OFF state. In the presence of Cu2+ and H2C2-swing arm, Cu+ was in situ generated quickly from the reduction of Cu2+ with the assistance of ascorbic acid, which could promptly and selectively trigger the Cu(I)-catalyzed click reaction-based 3D DNA walker between azide on N3-S1 and alkyne on the H2C2-swing arm. Sequentially, the activated H2C2-swing arm was able to hybridize with adjacent Cy3-HP and the 3D DNA walker was automatically driven by N.BstNBI to produce multiple Cy3-labeled DNA fragments away from the AuNP surface for signal amplification, performing a recovered fluorescence response (turning into the ON state). Accordingly, the ingenious integration of an efficient click reaction and smart 3D DNA walker endows the constructed fluorescent biosensor with superior selectivity and ultrahigh sensitivity. We further apply this platform for Cu2+ sensing in biological systems; this assay will provide a signal transduction strategy for evaluating intracellular Cu2+ at picomolar levels." @default.
• W3137184831 created "2021-03-29" @default.
• W3137184831 creator A5044096884 @default.
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• W3137184831 date "2021-03-23" @default.
• W3137184831 modified "2023-10-06" @default.
• W3137184831 title "Cu(I)-Catalyzed Click Reaction-Triggered 3D DNA Walker for Constructing an “OFF–ON” Fluorescent Biosensor for Cu²⁺ Detection" @default.
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• W3137184831 doi "https://doi.org/10.1021/acsabm.1c00073" @default.
• W3137184831 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35014442" @default.
• W3137184831 hasPublicationYear "2021" @default.
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