Matches in SemOpenAlex for { <https://semopenalex.org/work/W3137339922> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W3137339922 endingPage "114024" @default.
- W3137339922 startingPage "114024" @default.
- W3137339922 abstract "Lignosus rhinocerotis (Cooke) Ryvarden cultivar TM02, also known as tiger's milk mushroom, is regarded as important folk medicine in Malaysia, while is used for the treatment of liver cancer, chronic hepatitis, gastric ulcer in traditional Chinese medicine. However, there is no compilation of scientific evidence that its protection for gastric, and no attempts have been made to understand how polysaccharides in Lignosus rhinocerotis might promote intestinal mucosal wound healing. Aim of the study: This study aimed to investigate the effect and mechanism of β-glucan prepared from L. rhinocerotis using an enzymatic method on epithelial restitution during intestinal mucosal damage. Based on FT-IR, MALDI-TOF-MS, HPSEC-MALLS-RID, and AFM, the structure of polysaccharides from L. rhinocerotis was analysed. In addition, polysaccharides were used to test for wound healing activity in IEC-6 cells by measuring cell migration, proliferation, and expression of cell division control protein 42, Rac-1, RhoA, and Par-3. β-glucan was extracted using enzyme-assisted extraction, and a yield of approximately 8.5 ± 0.8% was obtained from the dried biomass. The β-glucan extracted by enzyme-assisted extraction (EAE) of polysaccharides was composed entirely of D-glucose with a total carbohydrate content of 95.5 ± 3.2%. The results of HPLC, FTIR, and MALDI-TOF-MS analyses revealed EAEP to be confirmed as β-glucan. The molecular weight of prepared β-glucan was found to be 5.315 × 104 g/mol by HPSEC-MALLS-RID. Furthermore, mucosal wound healing studies showed that the treatment of IEC-6 with a β-glucan concentration of 200 μg/mL promoted cell migration and proliferation, and it enhanced the protein expression of cell division control protein 42, Rac-1, RhoA, and Par-3. The present study reveals that the prepared β-glucan accelerates intestinal epithelial cell proliferation and migration via activation of Rho-dependent pathway. Hence, β-glucan can be employed as a prospective therapeutic agent for the treatment of diseases associated with gastrointestinal mucosal damage, such as peptic ulcers and inflammatory bowel disease." @default.
- W3137339922 created "2021-03-29" @default.
- W3137339922 creator A5015741466 @default.
- W3137339922 creator A5023363049 @default.
- W3137339922 creator A5029651848 @default.
- W3137339922 creator A5036728784 @default.
- W3137339922 creator A5047347967 @default.
- W3137339922 creator A5049417201 @default.
- W3137339922 creator A5071956372 @default.
- W3137339922 creator A5091184621 @default.
- W3137339922 date "2021-06-01" @default.
- W3137339922 modified "2023-09-24" @default.
- W3137339922 title "Restitution of epithelial cells during intestinal mucosal wound healing: The effect of a polysaccharide from the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden" @default.
- W3137339922 cites W1797745247 @default.
- W3137339922 cites W1964396187 @default.
- W3137339922 cites W1968834637 @default.
- W3137339922 cites W1969596974 @default.
- W3137339922 cites W1973263538 @default.
- W3137339922 cites W1975472487 @default.
- W3137339922 cites W1991509596 @default.
- W3137339922 cites W1993410470 @default.
- W3137339922 cites W2006431701 @default.
- W3137339922 cites W2013965332 @default.
- W3137339922 cites W2065490697 @default.
- W3137339922 cites W2069610733 @default.
- W3137339922 cites W2077855112 @default.
- W3137339922 cites W2079510851 @default.
- W3137339922 cites W2086951399 @default.
- W3137339922 cites W2134319370 @default.
- W3137339922 cites W2145416697 @default.
- W3137339922 cites W2158750799 @default.
- W3137339922 cites W2163962666 @default.
- W3137339922 cites W2164338584 @default.
- W3137339922 cites W2552439582 @default.
- W3137339922 cites W2569870625 @default.
- W3137339922 cites W2616387367 @default.
- W3137339922 cites W2775121423 @default.
- W3137339922 cites W2782268057 @default.
- W3137339922 cites W2783580373 @default.
- W3137339922 cites W2804891461 @default.
- W3137339922 cites W2807873974 @default.
- W3137339922 cites W2913513536 @default.
- W3137339922 cites W2937233919 @default.
- W3137339922 cites W2984286429 @default.
- W3137339922 cites W3002745423 @default.
- W3137339922 cites W3033930536 @default.
- W3137339922 cites W587615666 @default.
- W3137339922 doi "https://doi.org/10.1016/j.jep.2021.114024" @default.
- W3137339922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33727110" @default.
- W3137339922 hasPublicationYear "2021" @default.
- W3137339922 type Work @default.
- W3137339922 sameAs 3137339922 @default.
- W3137339922 citedByCount "11" @default.
- W3137339922 countsByYear W31373399222021 @default.
- W3137339922 countsByYear W31373399222022 @default.
- W3137339922 countsByYear W31373399222023 @default.
- W3137339922 crossrefType "journal-article" @default.
- W3137339922 hasAuthorship W3137339922A5015741466 @default.
- W3137339922 hasAuthorship W3137339922A5023363049 @default.
- W3137339922 hasAuthorship W3137339922A5029651848 @default.
- W3137339922 hasAuthorship W3137339922A5036728784 @default.
- W3137339922 hasAuthorship W3137339922A5047347967 @default.
- W3137339922 hasAuthorship W3137339922A5049417201 @default.
- W3137339922 hasAuthorship W3137339922A5071956372 @default.
- W3137339922 hasAuthorship W3137339922A5091184621 @default.
- W3137339922 hasConcept C100817775 @default.
- W3137339922 hasConcept C185592680 @default.
- W3137339922 hasConcept C203014093 @default.
- W3137339922 hasConcept C2776123893 @default.
- W3137339922 hasConcept C2780269544 @default.
- W3137339922 hasConcept C55493867 @default.
- W3137339922 hasConcept C86803240 @default.
- W3137339922 hasConceptScore W3137339922C100817775 @default.
- W3137339922 hasConceptScore W3137339922C185592680 @default.
- W3137339922 hasConceptScore W3137339922C203014093 @default.
- W3137339922 hasConceptScore W3137339922C2776123893 @default.
- W3137339922 hasConceptScore W3137339922C2780269544 @default.
- W3137339922 hasConceptScore W3137339922C55493867 @default.
- W3137339922 hasConceptScore W3137339922C86803240 @default.
- W3137339922 hasFunder F4320321001 @default.
- W3137339922 hasLocation W31373399221 @default.
- W3137339922 hasOpenAccess W3137339922 @default.
- W3137339922 hasPrimaryLocation W31373399221 @default.
- W3137339922 hasRelatedWork W155040066 @default.
- W3137339922 hasRelatedWork W1973356359 @default.
- W3137339922 hasRelatedWork W1995442432 @default.
- W3137339922 hasRelatedWork W2037302825 @default.
- W3137339922 hasRelatedWork W2038417614 @default.
- W3137339922 hasRelatedWork W2040820643 @default.
- W3137339922 hasRelatedWork W2065947667 @default.
- W3137339922 hasRelatedWork W2123206389 @default.
- W3137339922 hasRelatedWork W4323237708 @default.
- W3137339922 hasRelatedWork W2411280444 @default.
- W3137339922 hasVolume "274" @default.
- W3137339922 isParatext "false" @default.
- W3137339922 isRetracted "false" @default.
- W3137339922 magId "3137339922" @default.
- W3137339922 workType "article" @default.