FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3137441738> ?p ?o ?g. }

• W3137441738 endingPage "114513" @default.
• W3137441738 startingPage "114513" @default.
• W3137441738 abstract "Gentamicin (GM), an aminoglycoside antibiotic, is one commonly used clinical drugs with ototoxic side effects. One of the most principal mechanisms of its ototoxicity is that GM can activate caspase-mediated cell death pathways in the cochlea. Since the anti-apoptotic protein known as X-linked Inhibitor of Apoptosis Protein (XIAP) has been reported to directly bind to activated caspase protein and inhibit their activities, we hypothesized that it might protect cochlea hair cells from GM ototoxicity. To evaluate this hypothesis, postnatal day 2–3 (P2-3) transgenic (TG) mice, in which XIAP gene is over-expressed under a pure C57BL/6J genetic background was constructed. We first extracted the cochlea tissue of normal mice and treated them with different concentrations of GM, and the number of hair cells were observed to determine the concentration of GM used in subsequent experiments. Next, we used Western Blot experiment to examine the effect of GM on XIAP protein expression in normal mouse cochlea, and then Western Blot and RT-PCR experiments were used to identify the transgenic mice. Finally, immunofluorescence assays were used to detect the effect of GM on the expression of caspase protein and verify the protective effect of XIAP. We found that GM at a concentration of 0.5 mM significantly affected the function of cochlea hair cells, up-regulating the expression of cleaved-caspase-3 and cleaved-caspase-9 protein but down-regulating XIAP protein. In the cochlea tissues of TG mice, this effect of GM was suppressed, and the destruction of hair cells was significantly reduced, and the cleaved-caspase-3 and cleaved-caspase-9 proteins were significantly suppressed. These results suggested that XIAP reduces GM-induced ototoxicity and caspase-3/9 pathway is associated with this process." @default.
• W3137441738 created "2021-03-29" @default.
• W3137441738 creator A5016927477 @default.
• W3137441738 creator A5018331471 @default.
• W3137441738 creator A5018916657 @default.
• W3137441738 creator A5021525552 @default.
• W3137441738 creator A5024189861 @default.
• W3137441738 creator A5035935905 @default.
• W3137441738 creator A5046323357 @default.
• W3137441738 creator A5064876332 @default.
• W3137441738 creator A5067837433 @default.
• W3137441738 creator A5079842510 @default.
• W3137441738 date "2021-04-01" @default.
• W3137441738 modified "2023-09-23" @default.
• W3137441738 title "XIAP inhibits gentamicin-induced hair cell damage and ototoxicity through the caspase-3/9 pathway" @default.
• W3137441738 cites W1557686543 @default.
• W3137441738 cites W1980295286 @default.
• W3137441738 cites W1986622470 @default.
• W3137441738 cites W1993976861 @default.
• W3137441738 cites W1994558354 @default.
• W3137441738 cites W2005422662 @default.
• W3137441738 cites W2017053319 @default.
• W3137441738 cites W2025335830 @default.
• W3137441738 cites W2029431569 @default.
• W3137441738 cites W2033184553 @default.
• W3137441738 cites W2045114648 @default.
• W3137441738 cites W2059715997 @default.
• W3137441738 cites W2063385105 @default.
• W3137441738 cites W2080214438 @default.
• W3137441738 cites W2124775943 @default.
• W3137441738 cites W2144238086 @default.
• W3137441738 cites W2329794478 @default.
• W3137441738 cites W2518818230 @default.
• W3137441738 cites W2553343173 @default.
• W3137441738 cites W2590887400 @default.
• W3137441738 cites W2607403029 @default.
• W3137441738 cites W2759446432 @default.
• W3137441738 cites W2761759723 @default.
• W3137441738 cites W2765608133 @default.
• W3137441738 cites W2865806885 @default.
• W3137441738 cites W2889726478 @default.
• W3137441738 cites W2897987035 @default.
• W3137441738 cites W2901659440 @default.
• W3137441738 cites W2912121401 @default.
• W3137441738 cites W2921544063 @default.
• W3137441738 cites W2923900371 @default.
• W3137441738 cites W2943617019 @default.
• W3137441738 cites W2946529411 @default.
• W3137441738 cites W2994986743 @default.
• W3137441738 cites W3048852187 @default.
• W3137441738 cites W3087532860 @default.
• W3137441738 cites W3092309646 @default.
• W3137441738 cites W3112077480 @default.
• W3137441738 cites W4251632162 @default.
• W3137441738 doi "https://doi.org/10.1016/j.bcp.2021.114513" @default.
• W3137441738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33713642" @default.
• W3137441738 hasPublicationYear "2021" @default.
• W3137441738 type Work @default.
• W3137441738 sameAs 3137441738 @default.
• W3137441738 citedByCount "2" @default.
• W3137441738 countsByYear W31374417382022 @default.
• W3137441738 crossrefType "journal-article" @default.
• W3137441738 hasAuthorship W3137441738A5016927477 @default.
• W3137441738 hasAuthorship W3137441738A5018331471 @default.
• W3137441738 hasAuthorship W3137441738A5018916657 @default.
• W3137441738 hasAuthorship W3137441738A5021525552 @default.
• W3137441738 hasAuthorship W3137441738A5024189861 @default.
• W3137441738 hasAuthorship W3137441738A5035935905 @default.
• W3137441738 hasAuthorship W3137441738A5046323357 @default.
• W3137441738 hasAuthorship W3137441738A5064876332 @default.
• W3137441738 hasAuthorship W3137441738A5067837433 @default.
• W3137441738 hasAuthorship W3137441738A5079842510 @default.
• W3137441738 hasConcept C102230213 @default.
• W3137441738 hasConcept C104317684 @default.
• W3137441738 hasConcept C105702510 @default.
• W3137441738 hasConcept C12519072 @default.
• W3137441738 hasConcept C153911025 @default.
• W3137441738 hasConcept C185592680 @default.
• W3137441738 hasConcept C190283241 @default.
• W3137441738 hasConcept C2776415932 @default.
• W3137441738 hasConcept C2776694085 @default.
• W3137441738 hasConcept C2777408456 @default.
• W3137441738 hasConcept C2778239845 @default.
• W3137441738 hasConcept C2778534666 @default.
• W3137441738 hasConcept C2778585322 @default.
• W3137441738 hasConcept C2780130748 @default.
• W3137441738 hasConcept C31573885 @default.
• W3137441738 hasConcept C33195913 @default.
• W3137441738 hasConcept C54355233 @default.
• W3137441738 hasConcept C55493867 @default.
• W3137441738 hasConcept C85265743 @default.
• W3137441738 hasConcept C86803240 @default.
• W3137441738 hasConcept C95444343 @default.
• W3137441738 hasConcept C98424977 @default.
• W3137441738 hasConceptScore W3137441738C102230213 @default.
• W3137441738 hasConceptScore W3137441738C104317684 @default.
• W3137441738 hasConceptScore W3137441738C105702510 @default.
• W3137441738 hasConceptScore W3137441738C12519072 @default.

• next