FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3137704927> ?p ?o ?g. }

• W3137704927 endingPage "173" @default.
• W3137704927 startingPage "162" @default.
• W3137704927 abstract "From ancient times plants have been used for medicinal purposes against various ailments. In the modern era, plants are a major source of drugs and are an appealing drug candidate for the anticancer therapeutics against various molecular targets. Here we tested methanolic extract of dry leaves of Tamarix articulata for anticancer activity against a panel of hepatocellular carcinoma cells.Cell viability of hepatocellular carcinoma cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after a dose-dependent treatment with the extract of T. articulata. Phase-contrast microscopy and 4՛,6-diamidino-2-phenylindole (DAPI) staining served to analyse cellular and nuclear morphology. Immunoblotting was performed to determine the expression of proteins associated with autophagy, apoptosis and cell cycle. However, flow cytometry was used for the quantification of apoptotic cells and the analysis of cells in different phases of the cycle after the treatment with various doses of T. articulata. Additionally, acridine orange staining and 2՛,7՛-dichlorofluorescein diacetate (DCFH-DA) dye were used to analyse the quantification of autophagosomes and reactive oxygen species.Our results demonstrate that T. articulata methanolic extract exhibits promising antiproliferative activity with IC50 values (271.1±4.4), (298.3±7.1) and (336.7±6.1) µg/mL against hepatocellular carcinoma HepG2, Huh7D12 and Hep3B cell lines, respectively. Mechanistically, we found that T. articulata methanolic extract induces cell death by activating apoptosis and autophagy pathways. First, T. articulata methanolic extract promoted autophagy, which was confirmed by acridine orange staining. The immunoblotting analysis further confirmed that the extract at higher doses consistently induced the conversion of LC3I to LC3II form with a gradual decrease in the expression of autophagy substrate protein p62. Second, T. articulata methanolic extract promoted reactive oxygen species production in hepatocellular carcinoma cells and activated reactive oxygen species-mediated apoptosis. Flow cytometry and immunoblotting analysis showed that the plant methanolic extract induced dose-dependent apoptosis and activated proapoptotic proteins caspase-3 and PARP1. Additionally, the extract triggered the arrest of the G0/G1 phase of the cell cycle and upregulated the protein expression of p27/Kip and p21/Cip, with a decrease in cyclin D1 expression in hepatocellular carcinoma cells.The current study demonstrates that T. articulata methanolic extract exhibits promising anticancer potential to kill tumour cells by programmed cell death type I and II mechanisms and could be explored for potential drug candidate molecules to curtail cancer in the future." @default.
• W3137704927 created "2021-03-29" @default.
• W3137704927 creator A5045101383 @default.
• W3137704927 date "2021-03-23" @default.
• W3137704927 modified "2023-10-18" @default.
• W3137704927 title "Tamarix articulata Inhibits Cell Proliferation, Promotes Cell Death Mechanisms and Triggers G0/G1 Cell Cycle Arrest in Hepatocellular Carcinoma Cells" @default.
• W3137704927 cites W1482463156 @default.
• W3137704927 cites W1512072225 @default.
• W3137704927 cites W1854555833 @default.
• W3137704927 cites W1912388982 @default.
• W3137704927 cites W1965157031 @default.
• W3137704927 cites W1991412566 @default.
• W3137704927 cites W1995938892 @default.
• W3137704927 cites W2001751083 @default.
• W3137704927 cites W2033012486 @default.
• W3137704927 cites W2035756271 @default.
• W3137704927 cites W2046022351 @default.
• W3137704927 cites W2073582287 @default.
• W3137704927 cites W2074310251 @default.
• W3137704927 cites W2075700277 @default.
• W3137704927 cites W2077640619 @default.
• W3137704927 cites W2082289906 @default.
• W3137704927 cites W2082880246 @default.
• W3137704927 cites W2094085828 @default.
• W3137704927 cites W2124130126 @default.
• W3137704927 cites W2148469650 @default.
• W3137704927 cites W2158378236 @default.
• W3137704927 cites W2226263492 @default.
• W3137704927 cites W2234453634 @default.
• W3137704927 cites W2244276231 @default.
• W3137704927 cites W2325984664 @default.
• W3137704927 cites W2343898601 @default.
• W3137704927 cites W2406333788 @default.
• W3137704927 cites W2411266970 @default.
• W3137704927 cites W2508455866 @default.
• W3137704927 cites W2530765548 @default.
• W3137704927 cites W2533070124 @default.
• W3137704927 cites W2569285840 @default.
• W3137704927 cites W2601930952 @default.
• W3137704927 cites W2619578343 @default.
• W3137704927 cites W2748787958 @default.
• W3137704927 cites W2779780286 @default.
• W3137704927 cites W2789001259 @default.
• W3137704927 cites W2791551035 @default.
• W3137704927 cites W2889646458 @default.
• W3137704927 cites W2903786812 @default.
• W3137704927 cites W2908176514 @default.
• W3137704927 cites W2922569767 @default.
• W3137704927 cites W2961834959 @default.
• W3137704927 cites W3030409727 @default.
• W3137704927 cites W920673894 @default.
• W3137704927 doi "https://doi.org/10.17113/ftb.59.02.21.6904" @default.
• W3137704927 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8284106" @default.
• W3137704927 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34316277" @default.
• W3137704927 hasPublicationYear "2021" @default.
• W3137704927 type Work @default.
• W3137704927 sameAs 3137704927 @default.
• W3137704927 citedByCount "3" @default.
• W3137704927 countsByYear W31377049272022 @default.
• W3137704927 countsByYear W31377049272023 @default.
• W3137704927 crossrefType "journal-article" @default.
• W3137704927 hasAuthorship W3137704927A5045101383 @default.
• W3137704927 hasBestOaLocation W31377049271 @default.
• W3137704927 hasConcept C105696609 @default.
• W3137704927 hasConcept C153911025 @default.
• W3137704927 hasConcept C185592680 @default.
• W3137704927 hasConcept C190283241 @default.
• W3137704927 hasConcept C203522944 @default.
• W3137704927 hasConcept C2777527632 @default.
• W3137704927 hasConcept C2779102032 @default.
• W3137704927 hasConcept C2779964046 @default.
• W3137704927 hasConcept C2780783641 @default.
• W3137704927 hasConcept C29537977 @default.
• W3137704927 hasConcept C31573885 @default.
• W3137704927 hasConcept C48349386 @default.
• W3137704927 hasConcept C53227056 @default.
• W3137704927 hasConcept C54355233 @default.
• W3137704927 hasConcept C553184892 @default.
• W3137704927 hasConcept C55493867 @default.
• W3137704927 hasConcept C556039675 @default.
• W3137704927 hasConcept C62112901 @default.
• W3137704927 hasConcept C71924100 @default.
• W3137704927 hasConcept C74864618 @default.
• W3137704927 hasConcept C86803240 @default.
• W3137704927 hasConcept C95444343 @default.
• W3137704927 hasConceptScore W3137704927C105696609 @default.
• W3137704927 hasConceptScore W3137704927C153911025 @default.
• W3137704927 hasConceptScore W3137704927C185592680 @default.
• W3137704927 hasConceptScore W3137704927C190283241 @default.
• W3137704927 hasConceptScore W3137704927C203522944 @default.
• W3137704927 hasConceptScore W3137704927C2777527632 @default.
• W3137704927 hasConceptScore W3137704927C2779102032 @default.
• W3137704927 hasConceptScore W3137704927C2779964046 @default.
• W3137704927 hasConceptScore W3137704927C2780783641 @default.
• W3137704927 hasConceptScore W3137704927C29537977 @default.
• W3137704927 hasConceptScore W3137704927C31573885 @default.
• W3137704927 hasConceptScore W3137704927C48349386 @default.
• W3137704927 hasConceptScore W3137704927C53227056 @default.

• next