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- W3137796113 abstract "Artificial tetraploid and octaploid strains were induced from the wild species of Drosera rotundifolia (2n=2x=20) and D. anglica (2n=4x=40), respectively. The optimal condition of colchicine-treatments for polyploid inductions was determined first. A flow cytometry (FCM) analysis showed that the highest mixoploid score of D. rotundifolia was 20% in the treatment of 0.3% for 2 days (d), or 0.5% for 3 d, while the highest mixoploid score of D. anglica was 20% in the treatment of 0.5% for 2 d. Next, to remove chimeric cells, adventitious bud inductions were carried out using the FCM-selected individuals in both species. One strain from a total of 360 colchicine-treated leaf explants in each species had pure chromosome-double numbers of 2n=40 (tetraploid) in D. rotundifolia and 2n=80 (octaploid) in D. anglica. In both species, the guard cell sizes of the chromosome-doubled strains were larger than those of the wild types. The leaves of the chromosome-doubled strains of D. rotundifolia were larger than those of the wild diploid D. rotundifolia, while the leaves of the chromosome-doubled strains of D. anglica were smaller than those of the wild tetraploid D. anglica." @default.
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- W3137796113 date "2021-03-25" @default.
- W3137796113 modified "2023-09-27" @default.
- W3137796113 title "Colchicine Induction of Tetraploid and Octaploid <i>Drosera</i> Strains from <i>D. rotundifolia</i> and <i>D. anglica</i>" @default.
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- W3137796113 doi "https://doi.org/10.1508/cytologia.86.21" @default.
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