Matches in SemOpenAlex for { <https://semopenalex.org/work/W3137820144> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W3137820144 endingPage "390" @default.
- W3137820144 startingPage "390" @default.
- W3137820144 abstract "390 Lymphogenous spread is the first step of tumor progression and the extent of lymph node metastasis is a crucial prognostic parameter in patients with lung cancer. To clarify the key genes that are concerned in lymph node metastasis of lung cancer, we established two cell lines which metastasize to mediastinal lymph node in high and low frequency from a parental lung cancer cell line by in vivo selection and performed cDNA microarray analysis of these cell lines. In our previous experiment, after Ma44-3, lung cancer cell line, were orthotopically implanted to severe combined immunodeficiency (SCID) mice, macroscopically detectable tumor was formed at the site of injection on day 9 and mediastinal lymph node metastasis was observed on day 12. Using this model, lung tumors were harvested for culturing and implanted to the next generation mice. In the same way, metastatic mediastinal lymph nodes were passaged. These two passages were repeated five times. We established Ma44-3L from the lung tumor passage and Ma-44M from the mediastinal lymph node passage. These isolated cells were orthotopically implanted to each by five SCID mice to evaluate the potential of metastasis. The mice were sacrificed 2 weeks after implantation. Maximal diameter of the lung tumors and presence of mediastinal lymph node metastasis were evaluated. These two cell lines were subjected to cDNA microarray analysis followed by data mining studies. The lung tumors of Ma44-3L were significantly larger than that of Ma44-3M (3.28 ± 1.12 mm versus 1.74 ± 0.62 mm, p = 0.03). Ma44-3M metastasized to mediastinal lymph node in high frequency compared with Ma44-3L (5/5 versus 1/5, p = 0.01). We identified 79 genes that were 6-fold differentially expressed in Ma44-3L and Ma44-3M by cDNA microarray containing 14551 known genes. Genes upregulated in Ma44-3M cells included thrombospondin 1 and CD33 antigen, etc. that had been previously reported to be associated to metastasis of cancer cells. In conclusion, we established new cell lines with different potential to metastasize to mediastinal lymph node by in vivo selection. The candidate genes listed up by cDNA microarray may include key genes which are associated to important roles in lymph node metastasis in patients with lung cancer." @default.
- W3137820144 created "2021-03-29" @default.
- W3137820144 creator A5028866713 @default.
- W3137820144 creator A5038267674 @default.
- W3137820144 creator A5041031153 @default.
- W3137820144 creator A5046452323 @default.
- W3137820144 creator A5075648700 @default.
- W3137820144 creator A5076042448 @default.
- W3137820144 creator A5088408078 @default.
- W3137820144 date "2008-05-01" @default.
- W3137820144 modified "2023-09-24" @default.
- W3137820144 title "Novel lung cancer cell lines, Ma44-3L and Ma44-3M established by in vivo selection reveal candidate genes for lymph node metastasis" @default.
- W3137820144 hasPublicationYear "2008" @default.
- W3137820144 type Work @default.
- W3137820144 sameAs 3137820144 @default.
- W3137820144 citedByCount "0" @default.
- W3137820144 crossrefType "journal-article" @default.
- W3137820144 hasAuthorship W3137820144A5028866713 @default.
- W3137820144 hasAuthorship W3137820144A5038267674 @default.
- W3137820144 hasAuthorship W3137820144A5041031153 @default.
- W3137820144 hasAuthorship W3137820144A5046452323 @default.
- W3137820144 hasAuthorship W3137820144A5075648700 @default.
- W3137820144 hasAuthorship W3137820144A5076042448 @default.
- W3137820144 hasAuthorship W3137820144A5088408078 @default.
- W3137820144 hasConcept C121608353 @default.
- W3137820144 hasConcept C126322002 @default.
- W3137820144 hasConcept C142724271 @default.
- W3137820144 hasConcept C150903083 @default.
- W3137820144 hasConcept C207001950 @default.
- W3137820144 hasConcept C2776256026 @default.
- W3137820144 hasConcept C2777714996 @default.
- W3137820144 hasConcept C2779013556 @default.
- W3137820144 hasConcept C2779720271 @default.
- W3137820144 hasConcept C2780379385 @default.
- W3137820144 hasConcept C2780849966 @default.
- W3137820144 hasConcept C502942594 @default.
- W3137820144 hasConcept C71924100 @default.
- W3137820144 hasConcept C86803240 @default.
- W3137820144 hasConceptScore W3137820144C121608353 @default.
- W3137820144 hasConceptScore W3137820144C126322002 @default.
- W3137820144 hasConceptScore W3137820144C142724271 @default.
- W3137820144 hasConceptScore W3137820144C150903083 @default.
- W3137820144 hasConceptScore W3137820144C207001950 @default.
- W3137820144 hasConceptScore W3137820144C2776256026 @default.
- W3137820144 hasConceptScore W3137820144C2777714996 @default.
- W3137820144 hasConceptScore W3137820144C2779013556 @default.
- W3137820144 hasConceptScore W3137820144C2779720271 @default.
- W3137820144 hasConceptScore W3137820144C2780379385 @default.
- W3137820144 hasConceptScore W3137820144C2780849966 @default.
- W3137820144 hasConceptScore W3137820144C502942594 @default.
- W3137820144 hasConceptScore W3137820144C71924100 @default.
- W3137820144 hasConceptScore W3137820144C86803240 @default.
- W3137820144 hasLocation W31378201441 @default.
- W3137820144 hasOpenAccess W3137820144 @default.
- W3137820144 hasPrimaryLocation W31378201441 @default.
- W3137820144 hasRelatedWork W1588908451 @default.
- W3137820144 hasRelatedWork W1963482129 @default.
- W3137820144 hasRelatedWork W2016478357 @default.
- W3137820144 hasRelatedWork W2019798513 @default.
- W3137820144 hasRelatedWork W2030515135 @default.
- W3137820144 hasRelatedWork W2036805228 @default.
- W3137820144 hasRelatedWork W2090946772 @default.
- W3137820144 hasRelatedWork W2139350138 @default.
- W3137820144 hasRelatedWork W2162768149 @default.
- W3137820144 hasRelatedWork W2216293050 @default.
- W3137820144 hasRelatedWork W2317439266 @default.
- W3137820144 hasRelatedWork W2328204746 @default.
- W3137820144 hasRelatedWork W2341839242 @default.
- W3137820144 hasRelatedWork W2349037106 @default.
- W3137820144 hasRelatedWork W2886599779 @default.
- W3137820144 hasRelatedWork W2947923809 @default.
- W3137820144 hasRelatedWork W3013299851 @default.
- W3137820144 hasRelatedWork W3031754404 @default.
- W3137820144 hasRelatedWork W3213588690 @default.
- W3137820144 hasRelatedWork W72251609 @default.
- W3137820144 hasVolume "68" @default.
- W3137820144 isParatext "false" @default.
- W3137820144 isRetracted "false" @default.
- W3137820144 magId "3137820144" @default.
- W3137820144 workType "article" @default.