FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3138069613> ?p ?o ?g. }

• W3138069613 endingPage "105" @default.
• W3138069613 startingPage "97" @default.
• W3138069613 abstract "Abstract Malate dehydrogenase (MDH) catalyzes the reversible reduction of oxaloacetate (OAA) to L-malate using nicotinamide adenine dinucleotide hydrogen. MDH has two characteristic loops, the mobile loop and the catalytic loop, in the active site. On binding to the substrate, the enzyme undergoes a structural change from the open-form, with an open conformation of the mobile loop, to the closed-form, with the loop in a closed conformation. In this study, three crystals of MDH from a moderate thermophile, Geobacillus stearothermophilus (gs-MDH) were used to determine four different enzyme structures (resolutions, 1.95–2.20 Å), each of which was correspondingly assigned to its four catalytic states. Two OAA-unbound structures exhibited the open-form, while the other two OAA-bound structures exhibited both the open- and closed-form. The structural analysis suggested that the binding of OAA to the open-form gs-MDH promotes conformational change in the mobile loop and simultaneously activates the catalytic loop. The mutations on the key amino acid residues involving the proposed catalytic mechanism significantly affected the gs-MDH activity, supporting our hypothesis. These findings contribute to the elucidation of the detailed molecular mechanism underlying the substrate recognition and structural switching during the MDH catalytic cycle." @default.
• W3138069613 created "2021-03-29" @default.
• W3138069613 creator A5039811057 @default.
• W3138069613 creator A5063586448 @default.
• W3138069613 creator A5085078164 @default.
• W3138069613 creator A5090472475 @default.
• W3138069613 date "2021-03-16" @default.
• W3138069613 modified "2023-09-25" @default.
• W3138069613 title "Structural analysis and reaction mechanism of malate dehydrogenase from <i>Geobacillus stearothermophilus</i>" @default.
• W3138069613 cites W1486688369 @default.
• W3138069613 cites W1560368219 @default.
• W3138069613 cites W1588440265 @default.
• W3138069613 cites W1656293431 @default.
• W3138069613 cites W1987549377 @default.
• W3138069613 cites W1988790695 @default.
• W3138069613 cites W2001136648 @default.
• W3138069613 cites W2006175238 @default.
• W3138069613 cites W2017225435 @default.
• W3138069613 cites W2030419362 @default.
• W3138069613 cites W2032961491 @default.
• W3138069613 cites W2035436553 @default.
• W3138069613 cites W2038840577 @default.
• W3138069613 cites W2054576968 @default.
• W3138069613 cites W2061852108 @default.
• W3138069613 cites W2097493124 @default.
• W3138069613 cites W2100186628 @default.
• W3138069613 cites W2102028093 @default.
• W3138069613 cites W2110808180 @default.
• W3138069613 cites W2124026197 @default.
• W3138069613 cites W2145465660 @default.
• W3138069613 cites W2146460113 @default.
• W3138069613 cites W2147874841 @default.
• W3138069613 cites W2159211495 @default.
• W3138069613 cites W2162367618 @default.
• W3138069613 cites W2269101746 @default.
• W3138069613 cites W2275320080 @default.
• W3138069613 cites W2317505942 @default.
• W3138069613 cites W2808032448 @default.
• W3138069613 cites W2889587114 @default.
• W3138069613 cites W2900121089 @default.
• W3138069613 cites W2909198442 @default.
• W3138069613 cites W4293247451 @default.
• W3138069613 doi "https://doi.org/10.1093/jb/mvab027" @default.
• W3138069613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33723609" @default.
• W3138069613 hasPublicationYear "2021" @default.
• W3138069613 type Work @default.
• W3138069613 sameAs 3138069613 @default.
• W3138069613 citedByCount "3" @default.
• W3138069613 countsByYear W31380696132021 @default.
• W3138069613 countsByYear W31380696132022 @default.
• W3138069613 countsByYear W31380696132023 @default.
• W3138069613 crossrefType "journal-article" @default.
• W3138069613 hasAuthorship W3138069613A5039811057 @default.
• W3138069613 hasAuthorship W3138069613A5063586448 @default.
• W3138069613 hasAuthorship W3138069613A5085078164 @default.
• W3138069613 hasAuthorship W3138069613A5090472475 @default.
• W3138069613 hasConcept C111133373 @default.
• W3138069613 hasConcept C132901563 @default.
• W3138069613 hasConcept C181199279 @default.
• W3138069613 hasConcept C185592680 @default.
• W3138069613 hasConcept C2776901170 @default.
• W3138069613 hasConcept C2777339483 @default.
• W3138069613 hasConcept C27911776 @default.
• W3138069613 hasConcept C2909197731 @default.
• W3138069613 hasConcept C41183919 @default.
• W3138069613 hasConcept C55493867 @default.
• W3138069613 hasConcept C63338738 @default.
• W3138069613 hasConcept C71240020 @default.
• W3138069613 hasConcept C75520062 @default.
• W3138069613 hasConceptScore W3138069613C111133373 @default.
• W3138069613 hasConceptScore W3138069613C132901563 @default.
• W3138069613 hasConceptScore W3138069613C181199279 @default.
• W3138069613 hasConceptScore W3138069613C185592680 @default.
• W3138069613 hasConceptScore W3138069613C2776901170 @default.
• W3138069613 hasConceptScore W3138069613C2777339483 @default.
• W3138069613 hasConceptScore W3138069613C27911776 @default.
• W3138069613 hasConceptScore W3138069613C2909197731 @default.
• W3138069613 hasConceptScore W3138069613C41183919 @default.
• W3138069613 hasConceptScore W3138069613C55493867 @default.
• W3138069613 hasConceptScore W3138069613C63338738 @default.
• W3138069613 hasConceptScore W3138069613C71240020 @default.
• W3138069613 hasConceptScore W3138069613C75520062 @default.
• W3138069613 hasIssue "1" @default.
• W3138069613 hasLocation W31380696131 @default.
• W3138069613 hasOpenAccess W3138069613 @default.
• W3138069613 hasPrimaryLocation W31380696131 @default.
• W3138069613 hasRelatedWork W1917272347 @default.
• W3138069613 hasRelatedWork W1931404067 @default.
• W3138069613 hasRelatedWork W1972298054 @default.
• W3138069613 hasRelatedWork W2001689758 @default.
• W3138069613 hasRelatedWork W2038962064 @default.
• W3138069613 hasRelatedWork W2259749963 @default.
• W3138069613 hasRelatedWork W2890247174 @default.
• W3138069613 hasRelatedWork W3138069613 @default.
• W3138069613 hasRelatedWork W3138924135 @default.
• W3138069613 hasRelatedWork W4303647332 @default.
• W3138069613 hasVolume "170" @default.
• W3138069613 isParatext "false" @default.

• next