FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3138093048> ?p ?o ?g. }

• W3138093048 endingPage "1603" @default.
• W3138093048 startingPage "1597" @default.
• W3138093048 abstract "Background Rapid point-of-care (POC) detection of Streptococcus equi subsp. equi (S. equi) would theoretically reduce the spread of strangles by identifying index and carrier horses. Hypothesis That the eqbE isothermal amplification (LAMP) assay, and the same eqbE LAMP assay tested in a microfluidic device format, are comparable to a triplex real-time quantitative polymerase chain reaction (qPCR) assay that is commonly used in diagnostic labs. Samples Sixty-eight guttural pouch lavage (GPL) specimens from horses recovering from strangles. Methods Guttural pouch lavage specimens were tested for S. equi retrospectively using the benchtop eqbE LAMP, the eqbE LAMP microfluidic device, and compared to the triplex qPCR, that detects 2 S. equi-specific genes, eqbE and SEQ2190, as the reference standard using the receiver operating characteristic area under the curve (ROC). Results The 27/68 specimens were positive by benchtop eqbE LAMP, 31/64 by eqbE LAMP microfluidic device, and 12/67 by triplex qPCR. Using the triplex PCR as the reference, the benchtop eqbE LAMP showed excellent discrimination (ROC Area = 0.813, 95% confidence interval [CI] = 0.711-0.915) as did the LAMP microfluidic device (ROC Area = 0.811, 95% CI = 0.529-0.782). There was no significant difference between the benchtop LAMP and LAMP microfluidic device (ROC Area 0.813 ± 0.055 vs 0.811 ± 0.034, P = .97). Conclusions The eqbE LAMP microfluidic device detected S. equi in GPL specimens from convalescent horses. This assay shows potential for development as a POC device for rapid, sensitive, accurate, and cost-efficient detection of S. equi." @default.
• W3138093048 created "2021-03-29" @default.
• W3138093048 creator A5026531441 @default.
• W3138093048 creator A5036971008 @default.
• W3138093048 creator A5037039652 @default.
• W3138093048 creator A5039612950 @default.
• W3138093048 creator A5042585416 @default.
• W3138093048 creator A5046635151 @default.
• W3138093048 creator A5086106021 @default.
• W3138093048 date "2021-03-17" @default.
• W3138093048 modified "2023-10-14" @default.
• W3138093048 title "Detection of <i>Streptococcus equi</i> subsp. <i>equi</i> in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device" @default.
• W3138093048 cites W1989144306 @default.
• W3138093048 cites W1997925861 @default.
• W3138093048 cites W2020054214 @default.
• W3138093048 cites W2022013281 @default.
• W3138093048 cites W2023764483 @default.
• W3138093048 cites W2026493953 @default.
• W3138093048 cites W2049876707 @default.
• W3138093048 cites W2085254036 @default.
• W3138093048 cites W2087913566 @default.
• W3138093048 cites W2102704192 @default.
• W3138093048 cites W2105275554 @default.
• W3138093048 cites W2154173280 @default.
• W3138093048 cites W2164238430 @default.
• W3138093048 cites W2253475943 @default.
• W3138093048 cites W2304095541 @default.
• W3138093048 cites W2420917578 @default.
• W3138093048 cites W2603347257 @default.
• W3138093048 cites W2774413025 @default.
• W3138093048 cites W2793210890 @default.
• W3138093048 cites W2794202725 @default.
• W3138093048 cites W3009142941 @default.
• W3138093048 doi "https://doi.org/10.1111/jvim.16105" @default.
• W3138093048 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8163136" @default.
• W3138093048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33728675" @default.
• W3138093048 hasPublicationYear "2021" @default.
• W3138093048 type Work @default.
• W3138093048 sameAs 3138093048 @default.
• W3138093048 citedByCount "7" @default.
• W3138093048 countsByYear W31380930482022 @default.
• W3138093048 countsByYear W31380930482023 @default.
• W3138093048 crossrefType "journal-article" @default.
• W3138093048 hasAuthorship W3138093048A5026531441 @default.
• W3138093048 hasAuthorship W3138093048A5036971008 @default.
• W3138093048 hasAuthorship W3138093048A5037039652 @default.
• W3138093048 hasAuthorship W3138093048A5039612950 @default.
• W3138093048 hasAuthorship W3138093048A5042585416 @default.
• W3138093048 hasAuthorship W3138093048A5046635151 @default.
• W3138093048 hasAuthorship W3138093048A5086106021 @default.
• W3138093048 hasBestOaLocation W31380930481 @default.
• W3138093048 hasConcept C153911025 @default.
• W3138093048 hasConcept C24107716 @default.
• W3138093048 hasConcept C54355233 @default.
• W3138093048 hasConcept C552990157 @default.
• W3138093048 hasConcept C60635243 @default.
• W3138093048 hasConcept C71924100 @default.
• W3138093048 hasConcept C86803240 @default.
• W3138093048 hasConcept C89423630 @default.
• W3138093048 hasConceptScore W3138093048C153911025 @default.
• W3138093048 hasConceptScore W3138093048C24107716 @default.
• W3138093048 hasConceptScore W3138093048C54355233 @default.
• W3138093048 hasConceptScore W3138093048C552990157 @default.
• W3138093048 hasConceptScore W3138093048C60635243 @default.
• W3138093048 hasConceptScore W3138093048C71924100 @default.
• W3138093048 hasConceptScore W3138093048C86803240 @default.
• W3138093048 hasConceptScore W3138093048C89423630 @default.
• W3138093048 hasFunder F4320306716 @default.
• W3138093048 hasFunder F4320310441 @default.
• W3138093048 hasIssue "3" @default.
• W3138093048 hasLocation W31380930481 @default.
• W3138093048 hasLocation W31380930482 @default.
• W3138093048 hasOpenAccess W3138093048 @default.
• W3138093048 hasPrimaryLocation W31380930481 @default.
• W3138093048 hasRelatedWork W1989776535 @default.
• W3138093048 hasRelatedWork W2323826182 @default.
• W3138093048 hasRelatedWork W2353822722 @default.
• W3138093048 hasRelatedWork W2589356716 @default.
• W3138093048 hasRelatedWork W2890039456 @default.
• W3138093048 hasRelatedWork W3008344029 @default.
• W3138093048 hasRelatedWork W3030571003 @default.
• W3138093048 hasRelatedWork W3159382670 @default.
• W3138093048 hasRelatedWork W3164831237 @default.
• W3138093048 hasRelatedWork W3194289742 @default.
• W3138093048 hasVolume "35" @default.
• W3138093048 isParatext "false" @default.
• W3138093048 isRetracted "false" @default.
• W3138093048 magId "3138093048" @default.
• W3138093048 workType "article" @default.