FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3138178552> ?p ?o ?g. }

• W3138178552 endingPage "e0009277" @default.
• W3138178552 startingPage "e0009277" @default.
• W3138178552 abstract "Background Giardia duodenalis is a leading cause of gastroenteritis worldwide. Humans are mainly infected by two different subtypes, i.e., assemblage A and B. Genotyping is hampered by allelic sequence heterozygosity (ASH) mainly in assemblage B, and by occurrence of mixed infections. Here we assessed the suitability of current genotyping protocols of G . duodenalis for epidemiological applications such as molecular tracing of transmission chains. Methodology/Principal findings Two G . duodenalis isolate collections, from an outpatient tropical medicine clinic and from several primary care laboratories, were characterized by assemblage-specific qPCR ( TIF , CATH gene loci) and a common multi locus sequence typing (MLST; TPI , BG , GDH gene loci). Assemblage A isolates were further typed at additional loci ( HCMP22547 , CID1 , RHP26 , HCMP6372 , DIS3 , NEK15411 ). Of 175/202 (86.6%) patients the G . duodenalis assemblage could be identified: Assemblages A 25/175 (14.3%), B 115/175 (65.7%) and A+B mixed 35/175 (20.0%). By incorporating allelic sequence heterozygosity in the analysis, the three marker MLST correctly identified 6/9 (66,7%) and 4/5 (80.0%) consecutive samples from chronic assemblage B infections in the two collections, respectively, and identified a cluster of five independent patients carrying assemblage B parasites of identical MLST type. Extended MLST for assemblage A altogether identified 5/6 (83,3%) consecutive samples from chronic assemblage A infections and 15 novel genotypes. Based on the observed A+B mixed infections it is estimated that only 75% and 50% of assemblage A or B only cases represent single strain infections, respectively. We demonstrate that typing results are consistent with this prediction. Conclusions/Significance Typing of assemblage A and B isolates with resolution for epidemiological applications is possible but requires separate genotyping protocols. The high frequency of multiple infections and their impact on typing results are findings with immediate consequences for result interpretation in this field." @default.
• W3138178552 created "2021-03-29" @default.
• W3138178552 creator A5000784501 @default.
• W3138178552 creator A5003339327 @default.
• W3138178552 creator A5012196058 @default.
• W3138178552 creator A5013059849 @default.
• W3138178552 creator A5015372813 @default.
• W3138178552 creator A5026382712 @default.
• W3138178552 creator A5042349359 @default.
• W3138178552 creator A5046256280 @default.
• W3138178552 creator A5062609782 @default.
• W3138178552 creator A5064865713 @default.
• W3138178552 creator A5067383632 @default.
• W3138178552 creator A5069200331 @default.
• W3138178552 creator A5073700728 @default.
• W3138178552 creator A5077550092 @default.
• W3138178552 creator A5080350850 @default.
• W3138178552 date "2021-03-25" @default.
• W3138178552 modified "2023-10-14" @default.
• W3138178552 title "Suitability of current typing procedures to identify epidemiologically linked human Giardia duodenalis isolates" @default.
• W3138178552 cites W1772697273 @default.
• W3138178552 cites W1968644762 @default.
• W3138178552 cites W1986699044 @default.
• W3138178552 cites W1987038031 @default.
• W3138178552 cites W2019858741 @default.
• W3138178552 cites W2028881074 @default.
• W3138178552 cites W2041677458 @default.
• W3138178552 cites W2065655458 @default.
• W3138178552 cites W2068453849 @default.
• W3138178552 cites W2069976801 @default.
• W3138178552 cites W2071052938 @default.
• W3138178552 cites W2071615747 @default.
• W3138178552 cites W2075683777 @default.
• W3138178552 cites W2075760071 @default.
• W3138178552 cites W2086930596 @default.
• W3138178552 cites W2103696537 @default.
• W3138178552 cites W2147043577 @default.
• W3138178552 cites W2159795561 @default.
• W3138178552 cites W2168543463 @default.
• W3138178552 cites W2192659091 @default.
• W3138178552 cites W2511231130 @default.
• W3138178552 cites W2595927711 @default.
• W3138178552 cites W2624665019 @default.
• W3138178552 cites W2775793257 @default.
• W3138178552 cites W2790341796 @default.
• W3138178552 cites W2799524357 @default.
• W3138178552 cites W2947895020 @default.
• W3138178552 cites W2995259176 @default.
• W3138178552 cites W3005466491 @default.
• W3138178552 cites W3020077371 @default.
• W3138178552 doi "https://doi.org/10.1371/journal.pntd.0009277" @default.
• W3138178552 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8023459" @default.
• W3138178552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33764999" @default.
• W3138178552 hasPublicationYear "2021" @default.
• W3138178552 type Work @default.
• W3138178552 sameAs 3138178552 @default.
• W3138178552 citedByCount "15" @default.
• W3138178552 countsByYear W31381785522021 @default.
• W3138178552 countsByYear W31381785522022 @default.
• W3138178552 countsByYear W31381785522023 @default.
• W3138178552 crossrefType "journal-article" @default.
• W3138178552 hasAuthorship W3138178552A5000784501 @default.
• W3138178552 hasAuthorship W3138178552A5003339327 @default.
• W3138178552 hasAuthorship W3138178552A5012196058 @default.
• W3138178552 hasAuthorship W3138178552A5013059849 @default.
• W3138178552 hasAuthorship W3138178552A5015372813 @default.
• W3138178552 hasAuthorship W3138178552A5026382712 @default.
• W3138178552 hasAuthorship W3138178552A5042349359 @default.
• W3138178552 hasAuthorship W3138178552A5046256280 @default.
• W3138178552 hasAuthorship W3138178552A5062609782 @default.
• W3138178552 hasAuthorship W3138178552A5064865713 @default.
• W3138178552 hasAuthorship W3138178552A5067383632 @default.
• W3138178552 hasAuthorship W3138178552A5069200331 @default.
• W3138178552 hasAuthorship W3138178552A5073700728 @default.
• W3138178552 hasAuthorship W3138178552A5077550092 @default.
• W3138178552 hasAuthorship W3138178552A5080350850 @default.
• W3138178552 hasBestOaLocation W31381785521 @default.
• W3138178552 hasConcept C104317684 @default.
• W3138178552 hasConcept C135763542 @default.
• W3138178552 hasConcept C143589142 @default.
• W3138178552 hasConcept C159047783 @default.
• W3138178552 hasConcept C180754005 @default.
• W3138178552 hasConcept C2777271458 @default.
• W3138178552 hasConcept C2781209916 @default.
• W3138178552 hasConcept C31467283 @default.
• W3138178552 hasConcept C54355233 @default.
• W3138178552 hasConcept C77434933 @default.
• W3138178552 hasConcept C84597430 @default.
• W3138178552 hasConcept C86803240 @default.
• W3138178552 hasConceptScore W3138178552C104317684 @default.
• W3138178552 hasConceptScore W3138178552C135763542 @default.
• W3138178552 hasConceptScore W3138178552C143589142 @default.
• W3138178552 hasConceptScore W3138178552C159047783 @default.
• W3138178552 hasConceptScore W3138178552C180754005 @default.
• W3138178552 hasConceptScore W3138178552C2777271458 @default.
• W3138178552 hasConceptScore W3138178552C2781209916 @default.
• W3138178552 hasConceptScore W3138178552C31467283 @default.
• W3138178552 hasConceptScore W3138178552C54355233 @default.

• next