FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3138269968> ?p ?o ?g. }

• W3138269968 abstract "Abstract Proteases are among the largest protein families and critical regulators of biochemical processes like apoptosis and blood coagulation. Knowledge of proteases has been expanded by the development of proteomic approaches, however, technology for multiplexed screening of proteases within native environments is currently lacking behind. Here we introduce a simple method to profile protease activity based on isolation of protease products from native lysates using a 96FASP filter, their analysis in a mass spectrometer and a custom data analysis pipeline. The method is significantly faster, cheaper, technically less demanding, easy to multiplex and produces accurate protease fingerprints. Using the blood cascade proteases as a case study, we obtain protease substrate profiles that can be used to map specificity, cleavage entropy and allosteric effects and to design protease probes. The data further show that protease substrate predictions enable the selection of potential physiological substrates for targeted validation in biochemical assays." @default.
• W3138269968 created "2021-03-29" @default.
• W3138269968 creator A5008098404 @default.
• W3138269968 creator A5011766022 @default.
• W3138269968 creator A5036463770 @default.
• W3138269968 creator A5038565479 @default.
• W3138269968 creator A5042266030 @default.
• W3138269968 creator A5050663659 @default.
• W3138269968 creator A5053361384 @default.
• W3138269968 creator A5070272273 @default.
• W3138269968 creator A5076106368 @default.
• W3138269968 creator A5076623682 @default.
• W3138269968 creator A5084663726 @default.
• W3138269968 creator A5084855553 @default.
• W3138269968 date "2021-03-16" @default.
• W3138269968 modified "2023-10-01" @default.
• W3138269968 title "Mapping specificity, cleavage entropy, allosteric changes and substrates of blood proteases in a high-throughput screen" @default.
• W3138269968 cites W1518197977 @default.
• W3138269968 cites W1569301169 @default.
• W3138269968 cites W1787366775 @default.
• W3138269968 cites W1907442748 @default.
• W3138269968 cites W1923386801 @default.
• W3138269968 cites W1966354329 @default.
• W3138269968 cites W1968178167 @default.
• W3138269968 cites W1969847251 @default.
• W3138269968 cites W1975290591 @default.
• W3138269968 cites W1978611499 @default.
• W3138269968 cites W1980745149 @default.
• W3138269968 cites W1985469117 @default.
• W3138269968 cites W1986794972 @default.
• W3138269968 cites W2001349696 @default.
• W3138269968 cites W2003029464 @default.
• W3138269968 cites W2005882400 @default.
• W3138269968 cites W2010303722 @default.
• W3138269968 cites W2012034410 @default.
• W3138269968 cites W2013546183 @default.
• W3138269968 cites W2014093205 @default.
• W3138269968 cites W2019387110 @default.
• W3138269968 cites W2021320002 @default.
• W3138269968 cites W2026599765 @default.
• W3138269968 cites W2027290833 @default.
• W3138269968 cites W2029476353 @default.
• W3138269968 cites W2032028280 @default.
• W3138269968 cites W2032069121 @default.
• W3138269968 cites W2036321220 @default.
• W3138269968 cites W2037199116 @default.
• W3138269968 cites W2038499892 @default.
• W3138269968 cites W2038916773 @default.
• W3138269968 cites W2041500314 @default.
• W3138269968 cites W2041686750 @default.
• W3138269968 cites W2042342668 @default.
• W3138269968 cites W2050756511 @default.
• W3138269968 cites W2053559643 @default.
• W3138269968 cites W2053963714 @default.
• W3138269968 cites W2063683800 @default.
• W3138269968 cites W2069508712 @default.
• W3138269968 cites W2074912245 @default.
• W3138269968 cites W2075503331 @default.
• W3138269968 cites W2079171916 @default.
• W3138269968 cites W2080752012 @default.
• W3138269968 cites W2086179867 @default.
• W3138269968 cites W2089845879 @default.
• W3138269968 cites W2091273971 @default.
• W3138269968 cites W2092236356 @default.
• W3138269968 cites W2096057003 @default.
• W3138269968 cites W2097033861 @default.
• W3138269968 cites W2106376891 @default.
• W3138269968 cites W2113979911 @default.
• W3138269968 cites W2114958077 @default.
• W3138269968 cites W2121897924 @default.
• W3138269968 cites W2122683221 @default.
• W3138269968 cites W2130166312 @default.
• W3138269968 cites W2149554987 @default.
• W3138269968 cites W2159000061 @default.
• W3138269968 cites W2159675211 @default.
• W3138269968 cites W2164118220 @default.
• W3138269968 cites W2169437650 @default.
• W3138269968 cites W2171229523 @default.
• W3138269968 cites W2180610470 @default.
• W3138269968 cites W2268048347 @default.
• W3138269968 cites W2274817843 @default.
• W3138269968 cites W2328805967 @default.
• W3138269968 cites W2343251287 @default.
• W3138269968 cites W2343972246 @default.
• W3138269968 cites W2413747597 @default.
• W3138269968 cites W2428899421 @default.
• W3138269968 cites W2519691041 @default.
• W3138269968 cites W2521399715 @default.
• W3138269968 cites W2544360569 @default.
• W3138269968 cites W2570942072 @default.
• W3138269968 cites W2734072351 @default.
• W3138269968 cites W2739415904 @default.
• W3138269968 cites W2751285400 @default.
• W3138269968 cites W2764035603 @default.
• W3138269968 cites W2766093920 @default.
• W3138269968 cites W2769449671 @default.
• W3138269968 cites W2770663469 @default.
• W3138269968 cites W2779381970 @default.
• W3138269968 cites W2799876761 @default.
• W3138269968 cites W2800333284 @default.

• next