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• W3138290230 startingPage "15031" @default.
• W3138290230 abstract "Enrichment of rare cancer cells from various cell mixtures for subsequent analysis or culture is essential for understanding cancer formation and progression. In particular, maintaining the viability of captured cancer cells and gently releasing them for relevant applications remain challenging for many reported methods. Here, a physically cross-linked deoxyribozyme (DNAzyme)-based hydrogel strategy was developed for the specific envelopment and release of targeted cancer cells, allowing the aptamer-guided capture, 3D envelopment, and Zn2+-dependent release of viable cancer cells. The DNAzyme hydrogel is constructed through the intertwinement and hybridization of two complementary DNAzyme strands located on two rolling circle amplification-synthesized ultralong DNA chains. The enveloping and separation of target cells were achieved during the formation of the DNAzyme hydrogel (sol–gel transition). Triggered by Zn2+, the encapsulated cells can be gently released from the dissociated DNAzyme hydrogel with high viability (gel–sol transition). Successful isolations of target cells from cancer cell mixtures and peripheral blood mononuclear cells (PBMC) were demonstrated. This method offers an attractive approach for the separation of target cancer cells for various downstream applications that require viable cells." @default.
• W3138290230 created "2021-03-29" @default.
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• W3138290230 date "2021-03-25" @default.
• W3138290230 modified "2023-10-14" @default.
• W3138290230 title "DNAzyme-Triggered Sol–Gel–Sol Transition of a Hydrogel Allows Target Cell Enrichment" @default.
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• W3138290230 doi "https://doi.org/10.1021/acsami.1c02262" @default.
• W3138290230 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33764744" @default.
• W3138290230 hasPublicationYear "2021" @default.
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