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• W3138294194 abstract "Received: 2020-10-07 Accepted: 2021-02-08 Available online: 2021-02-28 https://doi.org/10.15414/afz.2021.24.mi-apa.1-6 Pathogen-related 10 is a group of proteins derived from various plant species that contains homologues of Bet v 1, major birch-pollen allergen, which are able to cross-react with an IgEs of sensitive patients and invoke allergic reactions. Despite new sequencing technologies and the amount of data generated daily, PR-10 proteins have not been discovered in every plant species. To describe genomic variability by the -omics sciences, it is necessary to screen an undiscovered members of the group, map ypr-10 genes in genome and determine sequence variabilities. In this study Bet v 1 based amplified profile (BBAP) method was used to analyse the variability of 14 vegetable species (Brassica oleracea in 4 varieties) and to compare generated polymorphism among them. For all of the analysed species, a total number of generated amplicons was 162. The most different profiles were generated by Pastinaca sativa and Capsicum annuum, the highest similarity was between Apium graveolens and B. oleracea var. capitata. New type of genic molecular markers was applied successfully and proved to be an effective technique to map variability of PR-10 group. Keywords: PR-10, Bet v 1 homologue, vegetable, DNA marker, cross-reaction, allergy, genic molecular marker References ANDERSEN, M.B.S. et al. (2009). Identification of European Allergy Patterns to the Allergen Families PR-10, LTP, and Profilin from Rosaceae Fruits. Clinic Rev Allerg Immunol, 41(1), 4–19. BREITENEDER, H. and EBNER, CH.M.B. (2000). Molecular and biochemical classification of plant-derived food allergens. Molecular Mechanisms in Allergy and Clinical Immunology, 106(1), P27–36. CABALLERO, T. and MARTIN-ESTEBAN, M. (1998). Association between pollen hypersensitivity and edible vegetable allergy: a review. J Investig Allergol Clin Immunol., 8(1), 6–16. ELISYUTINA, O. et al. 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Sequential IgE epitope analysis of a birch pollen allergen (Bet v1) and an apple allergen (Mal d1). Allergology International, 50(1), 57–62. VARSHNEY R.K. (2010). Gene-based marker systems in plants: high throughput approaches for discovery and genotyping. In: Molecular techniques in crop improvement. The Netherlands: Springer, pp. 119–142. VARSHNEY et al. (2007). Genic molecular markers in plants: development and applications. In: Genomics-Assisted Crop Improvement: vol. 1: Genomics Approaches and Platforms. Dordrecht: Springer, pp. 13–29. WHITE, T.J. et al. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols a guide to methods and applications. San Diego: Academic Press, pp. 315–322. WISNIEWSKI, M. et al. (2004). Distribution and partial characterization of seasonally expressed proteins in different aged shoots and roots of ‘Loring’ peach (Prunus persica). Tree Physiol, 24(3), 339–345. ŽIAROVSKA, J. and ZELEŇAKOVA, L. (2016). Central and Eastern European spring pollen allergens and their expression analysisstate of the art. Diversity, 8(4), 1–11. ŽIAROVSKA, J. and ZELEŇAKOVA, L. (2019). Application of genomic data for PCR screening of Bet v 1 conserved sequence in clinically relevant plant species. In: Systems Biology. London: IntechOpen, pp. 65–82. ISBN 978-1-83880-803-7." @default.
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• W3138294194 title "Variability of DNA based amplicon profiles generated by Bet v 1 homologous among different vegetable species" @default.
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