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- W31383154 abstract "AbstractImmunohistochemistry comprises methods used to recognize tissue components as antigens in situ by means of directly or indirectly labeled antibodies, usually (but not always) derived from another species. When applied to cell preparations, the same methods are called immunocytochemistry (Fig. 1), although some authors also use this term for immunostaining of cellular components in tissue sections. Note that compared with immunostaining of vital cells in suspension or cultured monolayers, the sensitivity of immunohistochemical cell-surface staining is considerably reduced because of the decreased amounts of marker antigen represented by the cross-section of the plasma membrane. Also, for certain cellular markers examined in a tissue section, truly peripheral staining may be difficult to distinguish from a rim of cytoplasmic antigen expression. Immunohistochemistry (A) is performed on tissue sections whereas immunocytochemistry (B) is performed on cells in suspensions, smears, or monolayers. Antibodies do not penetrate the surface membrane of living cells; therefore, unequivocal peripheral staining is obtained only for cells in suspension or vital monolayers. With tissue sections or dried (and fixed) cells, it is difficult to distinguish peripheral from cytoplasmic immunostaining unless the antigen is a distinct surface membrane marker. KeywordsCeliac DiseaseMarker AntigenMurine mAbsImmunoenzyme MethodUnwanted InteractionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
- W31383154 created "2016-06-24" @default.
- W31383154 creator A5042575652 @default.
- W31383154 creator A5052605298 @default.
- W31383154 date "2003-11-14" @default.
- W31383154 modified "2023-09-26" @default.
- W31383154 title "Immunohistochemistry in Research and Diagnosis of Celiac Disease" @default.
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- W31383154 doi "https://doi.org/10.1385/1-59259-082-9:203" @default.
- W31383154 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21374442" @default.
- W31383154 hasPublicationYear "2003" @default.
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