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- W3138340295 abstract "Angiogenesis is a critical step in repair of tissue injury. The pattern recognition receptors (PRRs) recognize pathogen and damage associated molecular patterns (DAMPs) during injury and achieve host defense directly. However, the role of NLR family CARD domain containing 5 (NLRC5), an important member of PPRs, beyond host defense in angiogenesis during tissue repair remains unknown. Methods: In vitro, western blot and real-time PCR (RT-PCR) were used to detect the expression of NLRC5 in endothelial cells (ECs). Immunofluorescence microscopy was used to reveal the subcellular location of NLRC5 in ECs. Cell proliferation, wound healing, tube formation assays of ECs were performed to study the role of NLRC5 in angiogenesis. By using Tie2Cre-NLRC5flox/flox mice and bone marrow transplantation studies, we defined an EC-specific role for NLRC5 in angiogenesis. Mechanistically, co-immunoprecipitation studies and RNA sequencing indicated that signal transducer and activator of transcription 3 (STAT3) was the target of NLRC5 in the nucleus. And Co-IP was used to verify the specific domain of NLRC5 binding with STAT3. ChIP assay determined the genes regulated by interaction of STAT3 and NLRC5. Results: Knockdown of NLRC5 in vitro or in vivo inhibited pathological angiogenesis, but had no effect on physiological angiogenesis. NLRC5 was also identified to bind to STAT3 in the nucleus required the integrated death-domain and nucleotide-binding domain (DD+NACHT domain) of NLRC5. And the interaction of STAT3 and NLRC5 could enhance the transcription of angiopoietin-2 (Ang2) and cyclin D1 (CCND1) to participate in angiogenesis. Conclusions: In the ischemic microenvironment, NLRC5 protein accumulates in the nucleus of ECs and enhances STAT3 transcriptional activity for angiogenesis. These findings establish NLRC5 as a novel modulator of VEGFA signaling, providing a new target for angiogenic therapy to foster tissue regeneration." @default.
- W3138340295 created "2021-03-29" @default.
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- W3138340295 date "2021-01-01" @default.
- W3138340295 modified "2023-09-23" @default.
- W3138340295 title "The subcellular redistribution of NLRC5 promotes angiogenesis via interacting with STAT3 in endothelial cells" @default.
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- W3138340295 cites W1536422556 @default.
- W3138340295 cites W1551620887 @default.
- W3138340295 cites W1587650589 @default.
- W3138340295 cites W1629239351 @default.
- W3138340295 cites W1887074705 @default.
- W3138340295 cites W1953357409 @default.
- W3138340295 cites W1963788416 @default.
- W3138340295 cites W1966682269 @default.
- W3138340295 cites W1966977448 @default.
- W3138340295 cites W1967497206 @default.
- W3138340295 cites W1970308473 @default.
- W3138340295 cites W1971272503 @default.
- W3138340295 cites W1974714084 @default.
- W3138340295 cites W1982782750 @default.
- W3138340295 cites W1983166456 @default.
- W3138340295 cites W1985558958 @default.
- W3138340295 cites W1992912767 @default.
- W3138340295 cites W1995913922 @default.
- W3138340295 cites W2001937169 @default.
- W3138340295 cites W2004010971 @default.
- W3138340295 cites W2007840001 @default.
- W3138340295 cites W2010457001 @default.
- W3138340295 cites W2014596538 @default.
- W3138340295 cites W2017716833 @default.
- W3138340295 cites W2026104001 @default.
- W3138340295 cites W2029275361 @default.
- W3138340295 cites W2030797581 @default.
- W3138340295 cites W2042433863 @default.
- W3138340295 cites W2045384882 @default.
- W3138340295 cites W2045474542 @default.
- W3138340295 cites W2045716655 @default.
- W3138340295 cites W2047119613 @default.
- W3138340295 cites W2047580079 @default.
- W3138340295 cites W2052798287 @default.
- W3138340295 cites W2053847949 @default.
- W3138340295 cites W2059778390 @default.
- W3138340295 cites W2063846665 @default.
- W3138340295 cites W2067262890 @default.
- W3138340295 cites W2072669480 @default.
- W3138340295 cites W2074196937 @default.
- W3138340295 cites W2078841246 @default.
- W3138340295 cites W2084977288 @default.
- W3138340295 cites W2091125942 @default.
- W3138340295 cites W2091774189 @default.
- W3138340295 cites W2097056602 @default.
- W3138340295 cites W2098085902 @default.
- W3138340295 cites W2098474946 @default.
- W3138340295 cites W2100155537 @default.
- W3138340295 cites W2100382285 @default.
- W3138340295 cites W2103422355 @default.
- W3138340295 cites W2108133289 @default.
- W3138340295 cites W2112911107 @default.
- W3138340295 cites W2120957859 @default.
- W3138340295 cites W2122413073 @default.
- W3138340295 cites W2130410032 @default.
- W3138340295 cites W2141659416 @default.
- W3138340295 cites W2141816935 @default.
- W3138340295 cites W2143819289 @default.
- W3138340295 cites W2156713309 @default.
- W3138340295 cites W2174540735 @default.
- W3138340295 cites W2187672874 @default.
- W3138340295 cites W2336439489 @default.
- W3138340295 cites W2370999228 @default.
- W3138340295 cites W2612267236 @default.
- W3138340295 cites W2766555262 @default.
- W3138340295 cites W2782415666 @default.
- W3138340295 cites W2787917311 @default.
- W3138340295 cites W2908629632 @default.
- W3138340295 cites W2915355067 @default.
- W3138340295 cites W2921364413 @default.
- W3138340295 cites W2949013828 @default.
- W3138340295 cites W2954574532 @default.
- W3138340295 cites W3003275621 @default.
- W3138340295 cites W3092250464 @default.
- W3138340295 doi "https://doi.org/10.7150/thno.54473" @default.
- W3138340295 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7977449" @default.
- W3138340295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33754073" @default.
- W3138340295 hasPublicationYear "2021" @default.
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