Matches in SemOpenAlex for { <https://semopenalex.org/work/W3138403521> ?p ?o ?g. }
- W3138403521 endingPage "147" @default.
- W3138403521 startingPage "142" @default.
- W3138403521 abstract "Hyperketonemia is a common condition in early-lactation dairy cows that has been associated with an increase in the risk of infectious disease. Recent mouse studies have elucidated an anti-inflammatory effect of the ketone body β-hydroxybutyrate (BHB). Therefore, the objective of this study was to determine whether BHB altered inflammatory responses in macrophages challenged with the common mastitis pathogen Streptococcus uberis. A secondary objective was to determine whether the inflammatory response to the S. uberis challenge was dependent on whether BHB was present in the medium during the challenge (i.e., preconditioned vs. continuous treatment). Two cell culture experiments were conducted. In the first experiment, mouse macrophages (RAW 264.7 line) were preconditioned with BHB (0, 0.6, 1.2, and 1.8 mM) for 24 h; the medium was then replaced with a standard cell culture medium, and the cells were challenged or not with S. uberis for an additional 6 h. In the second experiment, a similar protocol was used; however, cells were preconditioned with BHB (0, 0.6, 1.2, and 1.8 mM) for 24 h, the medium was replaced with fresh medium containing the same concentration of BHB, and cells were either challenged or not with S. uberis for 6 h. In both experiments, relative transcript abundance of cell membrane receptors (Tlr2 and Gpr109a), cytokines (Il1b, Il10, Tnf, and Tgfb1), and chemokines (Cxcl2 and Ccl5) were determined using quantitative real-time PCR and normalized against the geometric mean of Hprt and B2m. Data were analyzed using a linear mixed model, and orthogonal contrasts were conducted to examine the effect of S. uberis challenge and BHB treatment. Streptococcus uberis activated the macrophages, noted by greater transcript abundance of analyzed genes. Intriguingly, in both experiments, the S. uberis challenge increased expression of Gpr109a, which encodes a receptor that is ligated by BHB. Paradoxically, preconditioning macrophages with BHB increased transcript abundance of the immunosuppressive cytokine Tgfb1 and increased that of the neutrophil chemoattractant Cxcl2. Preconditioning decreased Tlr2 and tended to decrease Il10 transcript abundance. In opposition to the preconditioning experiment, continuous treatment of BHB during the S. uberis challenge linearly increased abundance of Tlr2 and Il10 transcripts. Continuous BHB treatment also increased expression of Il1b. In conclusion, BHB treatment altered macrophage inflammatory responses during an S. uberis challenge; however, the direction of this response was dependent on whether BHB was added to the medium during the S. uberis challenge. Future studies should be conducted using bovine macrophages and in vivo approaches to examine BHB effects during an S. uberis challenge." @default.
- W3138403521 created "2021-03-29" @default.
- W3138403521 creator A5009252477 @default.
- W3138403521 creator A5033124702 @default.
- W3138403521 creator A5085296529 @default.
- W3138403521 date "2021-05-01" @default.
- W3138403521 modified "2023-10-10" @default.
- W3138403521 title "Diverging in vitro inflammatory responses toward Streptococcus uberis in mouse macrophages either preconditioned or continuously treated with β-hydroxybutyrate" @default.
- W3138403521 cites W162694006 @default.
- W3138403521 cites W1965804930 @default.
- W3138403521 cites W1979218309 @default.
- W3138403521 cites W1980172779 @default.
- W3138403521 cites W1981266729 @default.
- W3138403521 cites W1991205984 @default.
- W3138403521 cites W2020461422 @default.
- W3138403521 cites W2025094672 @default.
- W3138403521 cites W2027503551 @default.
- W3138403521 cites W2036551825 @default.
- W3138403521 cites W2047259732 @default.
- W3138403521 cites W2047898798 @default.
- W3138403521 cites W2052068421 @default.
- W3138403521 cites W2052587051 @default.
- W3138403521 cites W2064009992 @default.
- W3138403521 cites W2068858277 @default.
- W3138403521 cites W2070083785 @default.
- W3138403521 cites W2074686769 @default.
- W3138403521 cites W2083247310 @default.
- W3138403521 cites W2108244474 @default.
- W3138403521 cites W2112351720 @default.
- W3138403521 cites W2117303935 @default.
- W3138403521 cites W2136503478 @default.
- W3138403521 cites W2148158629 @default.
- W3138403521 cites W2153781524 @default.
- W3138403521 cites W2155881922 @default.
- W3138403521 cites W2224455896 @default.
- W3138403521 cites W2422208037 @default.
- W3138403521 cites W2428360703 @default.
- W3138403521 cites W2429297895 @default.
- W3138403521 cites W2433140427 @default.
- W3138403521 cites W2800946386 @default.
- W3138403521 cites W2914997630 @default.
- W3138403521 cites W3007056364 @default.
- W3138403521 cites W3044158286 @default.
- W3138403521 cites W3176510027 @default.
- W3138403521 cites W4313182480 @default.
- W3138403521 doi "https://doi.org/10.3168/jdsc.2020-0038" @default.
- W3138403521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36339507" @default.
- W3138403521 hasPublicationYear "2021" @default.
- W3138403521 type Work @default.
- W3138403521 sameAs 3138403521 @default.
- W3138403521 citedByCount "1" @default.
- W3138403521 countsByYear W31384035212021 @default.
- W3138403521 crossrefType "journal-article" @default.
- W3138403521 hasAuthorship W3138403521A5009252477 @default.
- W3138403521 hasAuthorship W3138403521A5033124702 @default.
- W3138403521 hasAuthorship W3138403521A5085296529 @default.
- W3138403521 hasBestOaLocation W31384035211 @default.
- W3138403521 hasConcept C13373296 @default.
- W3138403521 hasConcept C185592680 @default.
- W3138403521 hasConcept C203014093 @default.
- W3138403521 hasConcept C2776070231 @default.
- W3138403521 hasConcept C2776914184 @default.
- W3138403521 hasConcept C2778276568 @default.
- W3138403521 hasConcept C2779791424 @default.
- W3138403521 hasConcept C2781236682 @default.
- W3138403521 hasConcept C523546767 @default.
- W3138403521 hasConcept C54355233 @default.
- W3138403521 hasConcept C81885089 @default.
- W3138403521 hasConcept C86803240 @default.
- W3138403521 hasConcept C89423630 @default.
- W3138403521 hasConceptScore W3138403521C13373296 @default.
- W3138403521 hasConceptScore W3138403521C185592680 @default.
- W3138403521 hasConceptScore W3138403521C203014093 @default.
- W3138403521 hasConceptScore W3138403521C2776070231 @default.
- W3138403521 hasConceptScore W3138403521C2776914184 @default.
- W3138403521 hasConceptScore W3138403521C2778276568 @default.
- W3138403521 hasConceptScore W3138403521C2779791424 @default.
- W3138403521 hasConceptScore W3138403521C2781236682 @default.
- W3138403521 hasConceptScore W3138403521C523546767 @default.
- W3138403521 hasConceptScore W3138403521C54355233 @default.
- W3138403521 hasConceptScore W3138403521C81885089 @default.
- W3138403521 hasConceptScore W3138403521C86803240 @default.
- W3138403521 hasConceptScore W3138403521C89423630 @default.
- W3138403521 hasIssue "3" @default.
- W3138403521 hasLocation W31384035211 @default.
- W3138403521 hasLocation W31384035212 @default.
- W3138403521 hasLocation W31384035213 @default.
- W3138403521 hasOpenAccess W3138403521 @default.
- W3138403521 hasPrimaryLocation W31384035211 @default.
- W3138403521 hasRelatedWork W1987844375 @default.
- W3138403521 hasRelatedWork W2014531291 @default.
- W3138403521 hasRelatedWork W2071803098 @default.
- W3138403521 hasRelatedWork W2129428874 @default.
- W3138403521 hasRelatedWork W2135288246 @default.
- W3138403521 hasRelatedWork W2151038017 @default.
- W3138403521 hasRelatedWork W2121755527 @default.
- W3138403521 hasRelatedWork W2183688687 @default.
- W3138403521 hasRelatedWork W2327573171 @default.