FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3138452142> ?p ?o ?g. }

• W3138452142 endingPage "2763" @default.
• W3138452142 startingPage "2753" @default.
• W3138452142 abstract "The serotonergic system in the human brain modulates several physiological processes, and altered serotonergic neurotransmission has been implicated in the neuropathology of several psychiatric disorders. The study of serotonergic neurotransmission in psychiatry has long been restricted to animal models, but advances in cell reprogramming technology have enabled the generation of serotonergic neurons from patient-induced pluripotent stem cells (iPSCs). While iPSC-derived human serotonergic neurons offer the possibility to study serotonin (5-HT) release and uptake, particularly by 5-HT-modulating drugs such as selective serotonin reuptake inhibitors (SSRIs), a major limitation is the inability to reliably quantify 5-HT secreted from neurons in vitro. Herein, we address this technical gap via a novel sensing technology that couples 5-HT-specific DNA aptamers into nanopores (glass nanopipettes) with orifices of ~10 nm to detect 5-HT in complex neuronal culture medium with higher selectivity, sensitivity, and stability than existing methods. The 5-HT aptamers undergo conformational rearrangement upon target capture and serve as gatekeepers of ionic flux through the nanopipette opening. We generated human serotonergic neurons in vitro and detected secreted 5-HT using aptamer-coated nanopipettes in a low nanomolar range, with the possibility of detecting significantly lower (picomolar) concentrations. Furthermore, as a proof of concept, we treated human serotonergic neurons in vitro with the SSRI citalopram and detected a significant increase in extracellular 5-HT using the aptamer-modified nanopipettes. We demonstrate the utility of such methods for 5-HT detection, raising the possibility of fast quantification of neurotransmitters secreted from patient-derived live neuronal cells." @default.
• W3138452142 created "2021-03-29" @default.
• W3138452142 creator A5005779814 @default.
• W3138452142 creator A5006850186 @default.
• W3138452142 creator A5036139390 @default.
• W3138452142 creator A5051062804 @default.
• W3138452142 creator A5056763020 @default.
• W3138452142 creator A5072968010 @default.
• W3138452142 creator A5088612897 @default.
• W3138452142 date "2021-03-25" @default.
• W3138452142 modified "2023-10-14" @default.
• W3138452142 title "Sensing serotonin secreted from human serotonergic neurons using aptamer-modified nanopipettes" @default.
• W3138452142 cites W1564340322 @default.
• W3138452142 cites W1592986776 @default.
• W3138452142 cites W1828538530 @default.
• W3138452142 cites W1858743365 @default.
• W3138452142 cites W1932150911 @default.
• W3138452142 cites W1964575026 @default.
• W3138452142 cites W1991199510 @default.
• W3138452142 cites W1995982366 @default.
• W3138452142 cites W2003373601 @default.
• W3138452142 cites W2011720547 @default.
• W3138452142 cites W2022012719 @default.
• W3138452142 cites W2022774313 @default.
• W3138452142 cites W2025130503 @default.
• W3138452142 cites W2029080751 @default.
• W3138452142 cites W2030046699 @default.
• W3138452142 cites W2030502812 @default.
• W3138452142 cites W2050226999 @default.
• W3138452142 cites W2072038209 @default.
• W3138452142 cites W2079321730 @default.
• W3138452142 cites W2079402691 @default.
• W3138452142 cites W2092379136 @default.
• W3138452142 cites W2104525153 @default.
• W3138452142 cites W2105344046 @default.
• W3138452142 cites W2109572360 @default.
• W3138452142 cites W2116609429 @default.
• W3138452142 cites W2124903589 @default.
• W3138452142 cites W2125987139 @default.
• W3138452142 cites W2129140710 @default.
• W3138452142 cites W2134155795 @default.
• W3138452142 cites W2141157874 @default.
• W3138452142 cites W2148332776 @default.
• W3138452142 cites W2159689257 @default.
• W3138452142 cites W2178778635 @default.
• W3138452142 cites W2223493846 @default.
• W3138452142 cites W2318301515 @default.
• W3138452142 cites W2341690817 @default.
• W3138452142 cites W2346680365 @default.
• W3138452142 cites W2347032452 @default.
• W3138452142 cites W2490041812 @default.
• W3138452142 cites W2514319882 @default.
• W3138452142 cites W2522090547 @default.
• W3138452142 cites W2599049865 @default.
• W3138452142 cites W2622214442 @default.
• W3138452142 cites W2750493435 @default.
• W3138452142 cites W2775361234 @default.
• W3138452142 cites W2790580419 @default.
• W3138452142 cites W2791771448 @default.
• W3138452142 cites W2885362413 @default.
• W3138452142 cites W2889388596 @default.
• W3138452142 cites W2891599654 @default.
• W3138452142 cites W2901405468 @default.
• W3138452142 cites W2912102328 @default.
• W3138452142 cites W2924802475 @default.
• W3138452142 cites W2940465020 @default.
• W3138452142 cites W2948409401 @default.
• W3138452142 cites W2979986216 @default.
• W3138452142 cites W2989707678 @default.
• W3138452142 cites W2995991076 @default.
• W3138452142 cites W3006545887 @default.
• W3138452142 cites W3006989689 @default.
• W3138452142 cites W3016455486 @default.
• W3138452142 cites W3027153371 @default.
• W3138452142 cites W3036873398 @default.
• W3138452142 cites W3085634663 @default.
• W3138452142 cites W3132074539 @default.
• W3138452142 cites W3146171909 @default.
• W3138452142 doi "https://doi.org/10.1038/s41380-021-01066-5" @default.
• W3138452142 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33767349" @default.
• W3138452142 hasPublicationYear "2021" @default.
• W3138452142 type Work @default.
• W3138452142 sameAs 3138452142 @default.
• W3138452142 citedByCount "13" @default.
• W3138452142 countsByYear W31384521422021 @default.
• W3138452142 countsByYear W31384521422022 @default.
• W3138452142 countsByYear W31384521422023 @default.
• W3138452142 crossrefType "journal-article" @default.
• W3138452142 hasAuthorship W3138452142A5005779814 @default.
• W3138452142 hasAuthorship W3138452142A5006850186 @default.
• W3138452142 hasAuthorship W3138452142A5036139390 @default.
• W3138452142 hasAuthorship W3138452142A5051062804 @default.
• W3138452142 hasAuthorship W3138452142A5056763020 @default.
• W3138452142 hasAuthorship W3138452142A5072968010 @default.
• W3138452142 hasAuthorship W3138452142A5088612897 @default.
• W3138452142 hasBestOaLocation W31384521422 @default.
• W3138452142 hasConcept C128972844 @default.
• W3138452142 hasConcept C153911025 @default.

• next