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- W3138541125 abstract "Antibiotics from few culturable microorganisms have saved millions of lives since the 20th century. But with resistance formation, these compounds become increasingly ineffective, while the majority of microbial and with that chemical compound diversity remains inaccessible for cultivation and exploration. Culturing recalcitrant bacteria is a stochastic process. But conventional methods are limited to low throughput. By increasing (i) throughput and (ii) sensitivity by miniaturization, we innovate microbiological cultivation to comply with biological stochasticity. Here, we introduce a droplet-based microscale cultivation system, which is directly coupled to a high-throughput screening for antimicrobial activity prior to strain isolation. We demonstrate that highly parallelized in-droplet cultivation starting from single cells results in the cultivation of yet uncultured species and a significantly higher bacterial diversity than standard agar plate cultivation. Strains able to inhibit intact reporter strains were isolated from the system. A variety of antimicrobial compounds were detected for a selected potent antibiotic producer." @default.
- W3138541125 created "2021-03-29" @default.
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- W3138541125 date "2021-03-25" @default.
- W3138541125 modified "2023-10-03" @default.
- W3138541125 title "Highly parallelized droplet cultivation and prioritization of antibiotic producers from natural microbial communities" @default.
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- W3138541125 doi "https://doi.org/10.7554/elife.64774" @default.
- W3138541125 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8081529" @default.
- W3138541125 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33764297" @default.
- W3138541125 hasPublicationYear "2021" @default.
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