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- W3138613630 abstract "Mature seeds are rich in storage proteins, which not only affect the quality of seeds, but also the energy source of seed germination and seedling growth. In this study, a gene encoding a seed storage protein SSA3 from Arabidopsis thaliana was isolated. The AtSSA3 gene was expressed in Escherichia coli BL21 (DE3). The fusion protein pET-32a(+)-AtSSA3 was induced under the optimized conditions of 1mM IPTG and 25°C for 4h. Western blot analysis the optimal conditions of protein expression. This study provides a theoretical and practical basis for the further study of seed storage protein genes in the future." @default.
- W3138613630 created "2021-03-29" @default.
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- W3138613630 date "2021-01-01" @default.
- W3138613630 modified "2023-09-26" @default.
- W3138613630 title "Expression of a Seed Storage Protein AtSSA3 of <i>Arabidopsis thaliana</i> in <i>Escherichia coli</i>" @default.
- W3138613630 doi "https://doi.org/10.5376/msb.2021.12.0001" @default.
- W3138613630 hasPublicationYear "2021" @default.
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