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- W3138656089 abstract "Photoacoustic imaging is a high-resolution and high-contrast technique, which combines optical contrast with ultrasonic detection to map the distribution of the absorbing pigments in biological tissues. As an important branch of photoacoustic imaging, optical-resolution photoacoustic microscopy (OR-PAM) suffers from narrow depth-of-field (DoF), since the lateral resolution is determined by tight optical focusing. The small DoF will prevent OR-PAM to achieve large volumetric imaging. Here, we developed an ultrafast axial-scanning multifocus photoacoustic microscope with extended depth-of-field based on a tunable acoustic gradient lens (TAG) and fiber delay network. The TAG lens is used to high -speed focus-shift. And a fiber delay network consists of three optical fibers with different lengths is used to split a single laser pulse into three sub-pulses with different delay time. A function generator generates a sinusoidal signal to drive the TAG lens at an eigenmode. The focusing power of the TAG lens will exhibit a sinusoidal oscillation at the frequency of the driving signal. Then, the three sub-pulses synchronizes with three vibration states of the TAG lens, respectively. Finally, we can obtain three focuses with different depth in one A-line data acquisition to improve the DoF. The DoF we measured by a vertically tilted carbon fiber is eatimated to larger than 775 μm, which is ~ three times of that of single-focus PAM. The large DoF of large volumetric PAM was also verified by imaging a tungsten wire network. This system can achieve rapid and large-scale monitoring of physiological activities, which could expand the application of OR-PAM in biomedical researches." @default.
- W3138656089 created "2021-03-29" @default.
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- W3138656089 date "2021-03-22" @default.
- W3138656089 modified "2023-10-18" @default.
- W3138656089 title "Large volumetric optical-resolution photoacoustic microscopy based on a tunable acoustic gradient lens and fiber delay network" @default.
- W3138656089 doi "https://doi.org/10.1117/12.2581303" @default.
- W3138656089 hasPublicationYear "2021" @default.
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