Matches in SemOpenAlex for { <https://semopenalex.org/work/W3138930378> ?p ?o ?g. }
- W3138930378 endingPage "111437" @default.
- W3138930378 startingPage "111437" @default.
- W3138930378 abstract "THB1 is a monomeric truncated hemoglobin (TrHb) found in the cytoplasm of the green alga Chlamydomonas reinhardtii. The canonical heme coordination scheme in hemoglobins is a proximal histidine ligand and an open distal site. In THB1, the latter site is occupied by Lys53, which is likely to facilitate Fe(II)/Fe(III) redox cycling but hinders dioxygen binding, two features inherent to the NO dioxygenase activity of the protein. TrHb surveys show that a lysine at a position aligning with Lys53 is an insufficient determinant of coordination, and in this study, we sought to identify factors controlling lysine affinity for the heme iron. We solved the Lys-off X-ray structure of THB1, represented by the cyanide adduct of the Fe(III) protein, and hypothesized that interactions that differ between the known Lys-on structure and the Lys-off structure participate in the control of Lys53 affinity for the heme iron. We applied an experimental approach (site-directed mutagenesis, heme modification, pH titrations in the Fe(III) and Fe(II) states) and a computational approach (MD simulations in the Fe(II) state) to assess the role of heme propionate-protein interactions, distal helix capping, and the composition of the distal pocket. All THB1 modifications resulted in a weakening of lysine affinity and affected the coupling between Lys53 proton binding and heme redox potential. The results supported the importance of specific heme peripheral interactions for the pH stability of iron coordination and the ability of the protein to undergo redox reactions." @default.
- W3138930378 created "2021-03-29" @default.
- W3138930378 creator A5004937183 @default.
- W3138930378 creator A5018156342 @default.
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- W3138930378 creator A5048893233 @default.
- W3138930378 creator A5051187430 @default.
- W3138930378 creator A5083216120 @default.
- W3138930378 date "2021-06-01" @default.
- W3138930378 modified "2023-10-16" @default.
- W3138930378 title "Control of distal lysine coordination in a monomeric hemoglobin: A role for heme peripheral interactions" @default.
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