FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3139012805> ?p ?o ?g. }

• W3139012805 endingPage "774" @default.
• W3139012805 startingPage "766" @default.
• W3139012805 abstract "We recently described an orthogonal initiator tRNA (itRNATy2) that can initiate protein synthesis with noncanonical amino acids (ncAAs) in response to the UAG nonsense codon. Here, we report that a mutant of itRNATy2 (itRNATy2AUA) can efficiently initiate translation in response to the UAU tyrosine codon, giving rise to proteins with an ncAA at their N-terminus. We show that, in cells expressing itRNATy2AUA, UAU can function as a dual-use codon that selectively encodes ncAAs at the initiating position and predominantly tyrosine at elongating positions. Using itRNATy2AUA, in conjunction with its cognate tyrosyl-tRNA synthetase and two mutually orthogonal pyrrolysyl-tRNA synthetases, we demonstrate that UAU can be reassigned along with UAG or UAA to encode two distinct ncAAs in the same protein. Furthermore, by engineering the substrate specificity of one of the pyrrolysyl-tRNA synthetases, we developed a triply orthogonal system that enables simultaneous reassignment of UAU, UAG, and UAA to produce proteins containing three distinct ncAAs at precisely defined sites. To showcase the utility of this system, we produced proteins containing two or three ncAAs, with unique bioorthogonal functional groups, and demonstrate that these proteins can be separately modified with multiple fluorescent probes." @default.
• W3139012805 created "2021-03-29" @default.
• W3139012805 creator A5060076105 @default.
• W3139012805 creator A5062287329 @default.
• W3139012805 creator A5063450287 @default.
• W3139012805 creator A5067830024 @default.
• W3139012805 date "2021-03-16" @default.
• W3139012805 modified "2023-09-25" @default.
• W3139012805 title "Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons" @default.
• W3139012805 cites W1666992502 @default.
• W3139012805 cites W1916185738 @default.
• W3139012805 cites W1970652428 @default.
• W3139012805 cites W1972524605 @default.
• W3139012805 cites W1974357725 @default.
• W3139012805 cites W1989636060 @default.
• W3139012805 cites W1990148399 @default.
• W3139012805 cites W1995427715 @default.
• W3139012805 cites W2002336386 @default.
• W3139012805 cites W2006557567 @default.
• W3139012805 cites W2024960262 @default.
• W3139012805 cites W2029672674 @default.
• W3139012805 cites W2036284784 @default.
• W3139012805 cites W2045826940 @default.
• W3139012805 cites W2057892106 @default.
• W3139012805 cites W2061539493 @default.
• W3139012805 cites W2067826785 @default.
• W3139012805 cites W2069493520 @default.
• W3139012805 cites W2084922131 @default.
• W3139012805 cites W2090897179 @default.
• W3139012805 cites W2100475836 @default.
• W3139012805 cites W2106687445 @default.
• W3139012805 cites W2107167859 @default.
• W3139012805 cites W2111205496 @default.
• W3139012805 cites W2117449534 @default.
• W3139012805 cites W2137162349 @default.
• W3139012805 cites W2147565550 @default.
• W3139012805 cites W2156185678 @default.
• W3139012805 cites W2157680389 @default.
• W3139012805 cites W2169515696 @default.
• W3139012805 cites W2183760426 @default.
• W3139012805 cites W2261688117 @default.
• W3139012805 cites W2273680575 @default.
• W3139012805 cites W2322691084 @default.
• W3139012805 cites W2327644208 @default.
• W3139012805 cites W2334450392 @default.
• W3139012805 cites W2396732095 @default.
• W3139012805 cites W2418946485 @default.
• W3139012805 cites W2523671395 @default.
• W3139012805 cites W2542313139 @default.
• W3139012805 cites W2782374143 @default.
• W3139012805 cites W2789971571 @default.
• W3139012805 cites W2803722031 @default.
• W3139012805 cites W2883738486 @default.
• W3139012805 cites W2893257028 @default.
• W3139012805 cites W2923401475 @default.
• W3139012805 cites W2936502125 @default.
• W3139012805 cites W2995463657 @default.
• W3139012805 cites W3004786459 @default.
• W3139012805 cites W3032151504 @default.
• W3139012805 doi "https://doi.org/10.1021/acschembio.1c00120" @default.
• W3139012805 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8336083" @default.
• W3139012805 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33723984" @default.
• W3139012805 hasPublicationYear "2021" @default.
• W3139012805 type Work @default.
• W3139012805 sameAs 3139012805 @default.
• W3139012805 citedByCount "33" @default.
• W3139012805 countsByYear W31390128052021 @default.
• W3139012805 countsByYear W31390128052022 @default.
• W3139012805 countsByYear W31390128052023 @default.
• W3139012805 crossrefType "journal-article" @default.
• W3139012805 hasAuthorship W3139012805A5060076105 @default.
• W3139012805 hasAuthorship W3139012805A5062287329 @default.
• W3139012805 hasAuthorship W3139012805A5063450287 @default.
• W3139012805 hasAuthorship W3139012805A5067830024 @default.
• W3139012805 hasBestOaLocation W31390128053 @default.
• W3139012805 hasConcept C104317684 @default.
• W3139012805 hasConcept C10853874 @default.
• W3139012805 hasConcept C143065580 @default.
• W3139012805 hasConcept C153957851 @default.
• W3139012805 hasConcept C185592680 @default.
• W3139012805 hasConcept C20580545 @default.
• W3139012805 hasConcept C21951064 @default.
• W3139012805 hasConcept C2776165026 @default.
• W3139012805 hasConcept C2779315393 @default.
• W3139012805 hasConcept C515207424 @default.
• W3139012805 hasConcept C55493867 @default.
• W3139012805 hasConcept C67705224 @default.
• W3139012805 hasConcept C69610077 @default.
• W3139012805 hasConcept C70721500 @default.
• W3139012805 hasConcept C86803240 @default.
• W3139012805 hasConcept C930959 @default.
• W3139012805 hasConceptScore W3139012805C104317684 @default.
• W3139012805 hasConceptScore W3139012805C10853874 @default.
• W3139012805 hasConceptScore W3139012805C143065580 @default.
• W3139012805 hasConceptScore W3139012805C153957851 @default.
• W3139012805 hasConceptScore W3139012805C185592680 @default.
• W3139012805 hasConceptScore W3139012805C20580545 @default.
• W3139012805 hasConceptScore W3139012805C21951064 @default.

• next