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• W3139462012 abstract "In view of the global environmental and economic challenges of conventional organic solvents, most of the ionic liquids (ILs) have proved themselves as a “greener” group of cosolvents, especially for enzymes. However, there is a lack of fundamental knowledge on the greener aspects of various multicationic IL systems on biomolecules. In this regard, we have synthesized the monocholinium citrate ([Chn][Cit]), dicholinium citrate ([Chn]2[Cit]) and tricholinium citrate ([Chn]3[Cit]) ILs and employed them to explore their suitability for α-chymotrypsin (α-CT). This study specifically addresses the effect of increased cationic ratio of IL on stability and activity of α-CT with the help of various spectroscopic techniques. Various spectroscopic analysis showed that there is improved structural stability and activity of α-CT as the cationic ratio of IL increase. We found better structural stability of α-CT in [Chn]3[Cit] and [Chn]2[Cit] as compared to that in [Chn][Cit]. Interestingly, temperature-dependent fluorescence spectroscopy results explicitly elucidate that transition temperature (Tm) of α-CT enhances by 6 °C in the presence of [Chn]2[Cit] when compared to Tm of α-CT in buffer solution. Ultimately, these results establish that through an increase in the molar ratio of aqueous cholinium cation in [Chn][Cit] intensifies the capability of ILs to stabilize the α-CT structure. Our results suggested that multicationic IL system sensitivity to cosolvent exposure may be utlized in the future as a new approach to explore new biocompatibility toward proven stability studies." @default.
• W3139462012 created "2021-03-29" @default.
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• W3139462012 date "2021-03-25" @default.
• W3139462012 modified "2023-09-23" @default.
• W3139462012 title "Assessing the Compatibility of Mono-, Di-, and Tri-Cholinium Citrate Ionic Liquids for the Stability and Activity of α-Chymotrypsin" @default.
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• W3139462012 doi "https://doi.org/10.1021/acssuschemeng.1c00044" @default.
• W3139462012 hasPublicationYear "2021" @default.
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